Gene description for PHLDB2
Gene name pleckstrin homology-like domain, family B, member 2
Gene symbol PHLDB2
Other names/aliases LL5b
LL5beta
Species Homo sapiens
 Database cross references - PHLDB2
ExoCarta ExoCarta_90102
Vesiclepedia VP_90102
Entrez Gene 90102
HGNC 29573
MIM 610298
UniProt Q86SQ0  
 PHLDB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for PHLDB2
Molecular Function
    protein binding GO:0005515 IPI
    cadherin binding GO:0045296 HDA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IMP
    regulation of gastrulation GO:0010470 IMP
    regulation of epithelial to mesenchymal transition GO:0010717 IMP
    cell migration GO:0016477 IEA
    establishment of cell polarity GO:0030010 IEA
    establishment of protein localization GO:0045184 IMP
    negative regulation of stress fiber assembly GO:0051497 IMP
    negative regulation of focal adhesion assembly GO:0051895 IMP
    regulation of microtubule cytoskeleton organization GO:0070507 IBA
    regulation of microtubule cytoskeleton organization GO:0070507 IGI
    negative regulation of wound healing, spreading of epidermal cells GO:1903690 IMP
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 IGI
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 IMP
Subcellular Localization
    podosome GO:0002102 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 IDA
    cell cortex GO:0005938 IDA
    cell leading edge GO:0031252 IDA
    cell projection GO:0042995 IEA
    intermediate filament cytoskeleton GO:0045111 IDA
    basal cortex GO:0045180 IBA
    basal cortex GO:0045180 IDA
 Experiment description of studies that identified PHLDB2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHLDB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC4  
Affinity Capture-MS Homo sapiens
2 KIF23 9493
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CEP250 11190
Affinity Capture-MS Homo sapiens
4 OCLN 100506658
Proximity Label-MS Homo sapiens
5 KSR1  
Affinity Capture-MS Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 RACGAP1 29127
Affinity Capture-MS Homo sapiens
8 PARD3 56288
Proximity Label-MS Homo sapiens
9 Calml3  
Affinity Capture-MS Mus musculus
10 KIF20A 10112
Affinity Capture-MS Homo sapiens
11 CDC25B 994
Affinity Capture-MS Homo sapiens
12 SRGAP2 23380
Affinity Capture-MS Homo sapiens
13 CAMSAP2  
Affinity Capture-MS Homo sapiens
14 KIF7 374654
Affinity Capture-MS Homo sapiens
15 USP21  
Affinity Capture-MS Homo sapiens
16 Actb 11461
Affinity Capture-MS Mus musculus
17 EIF4E2  
Affinity Capture-MS Homo sapiens
18 KIF13B 23303
Affinity Capture-MS Homo sapiens
19 CAPZB 832
Affinity Capture-MS Homo sapiens
20 MLLT4 4301
Proximity Label-MS Homo sapiens
21 ZBTB21  
Affinity Capture-MS Homo sapiens
22 RASAL2 9462
Affinity Capture-MS Homo sapiens
23 ZNF638 27332
Affinity Capture-MS Homo sapiens
24 RAB3IP  
Affinity Capture-MS Homo sapiens
25 CALD1 800
Affinity Capture-MS Homo sapiens
26 CALM3 808
Affinity Capture-MS Homo sapiens
27 Ankrd26  
Affinity Capture-MS Mus musculus
28 FBXW8 26259
Affinity Capture-MS Homo sapiens
29 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 OBSL1 23363
Affinity Capture-MS Homo sapiens
31 TIAM1  
Affinity Capture-MS Homo sapiens
32 CBY1  
Affinity Capture-MS Homo sapiens
33 DYNLL1 8655
Affinity Capture-MS Homo sapiens
34 DCLK1 9201
Affinity Capture-MS Homo sapiens
35 TEX19  
Affinity Capture-MS Homo sapiens
36 YWHAH 7533
Affinity Capture-MS Homo sapiens
37 BRPF3  
Affinity Capture-MS Homo sapiens
38 LRFN1  
Affinity Capture-MS Homo sapiens
39 USP7 7874
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
40 DVL2 1856
Affinity Capture-MS Homo sapiens
41 KIAA1804  
Affinity Capture-MS Homo sapiens
42 KIF14 9928
Affinity Capture-MS Homo sapiens
43 HSPA8 3312
Affinity Capture-MS Homo sapiens
44 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 AGAP1  
Affinity Capture-MS Homo sapiens
46 E2F4  
Affinity Capture-MS Homo sapiens
47 GRIA1  
Affinity Capture-Western Homo sapiens
48 MYO18A 399687
Affinity Capture-MS Homo sapiens
49 ANKRD34A  
Affinity Capture-MS Homo sapiens
50 CTDP1 9150
Affinity Capture-MS Homo sapiens
51 FAM110A  
Affinity Capture-MS Homo sapiens
52 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
53 TESK2  
Affinity Capture-MS Homo sapiens
54 LYN 4067
Proximity Label-MS Homo sapiens
55 OFD1 8481
Affinity Capture-MS Homo sapiens
56 Ring1  
Affinity Capture-MS Mus musculus
57 CDH1 999
Proximity Label-MS Homo sapiens
58 YWHAE 7531
Affinity Capture-MS Homo sapiens
59 CCDC8  
Affinity Capture-MS Homo sapiens
60 YWHAQ 10971
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
61 Coro1c 23790
Affinity Capture-MS Mus musculus
62 FAM110B  
Affinity Capture-MS Homo sapiens
63 KCTD3  
Affinity Capture-MS Homo sapiens
64 PDGFRA 5156
Affinity Capture-MS Homo sapiens
65 PIPSL 266971
Affinity Capture-MS Homo sapiens
66 LIMA1 51474
Affinity Capture-MS Homo sapiens
67 DENND4C 55667
Affinity Capture-MS Homo sapiens
68 Myh9 17886
Affinity Capture-MS Mus musculus
69 Cd2ap 12488
Affinity Capture-MS Mus musculus
70 FLNA 2316
Affinity Capture-MS Homo sapiens
71 ECT2 1894
Affinity Capture-MS Homo sapiens
72 DLG4  
Affinity Capture-Western Homo sapiens
73 EPB41L4A 64097
Proximity Label-MS Homo sapiens
74 CTNNB1 1499
Affinity Capture-MS Homo sapiens
75 INPP5E 56623
Affinity Capture-MS Homo sapiens
76 TXLNB  
Affinity Capture-MS Homo sapiens
77 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
78 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 MYO19  
Affinity Capture-MS Homo sapiens
80 NADK 65220
Affinity Capture-MS Homo sapiens
81 MYO1C 4641
Affinity Capture-MS Homo sapiens
82 SYDE1 85360
Affinity Capture-MS Homo sapiens
83 CDK16 5127
Affinity Capture-MS Homo sapiens
84 KCNE3  
Affinity Capture-MS Homo sapiens
85 Cep72  
Affinity Capture-MS Mus musculus
86 SYNPO 11346
Affinity Capture-MS Homo sapiens
87 MYO6 4646
Proximity Label-MS Homo sapiens
88 MYC  
Affinity Capture-MS Homo sapiens
89 GIGYF1  
Affinity Capture-MS Homo sapiens
90 NDC80 10403
Proximity Label-MS Homo sapiens
91 CGN  
Affinity Capture-MS Homo sapiens
92 RHOB 388
Proximity Label-MS Homo sapiens
93 FLOT1 10211
Proximity Label-MS Homo sapiens
94 C10orf2 56652
Affinity Capture-MS Homo sapiens
95 FTL 2512
Affinity Capture-MS Homo sapiens
96 GIGYF2 26058
Affinity Capture-MS Homo sapiens
97 Tmod3 50875
Affinity Capture-MS Mus musculus
98 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
99 FAM53C 51307
Affinity Capture-MS Homo sapiens
100 RTKN 6242
Affinity Capture-MS Homo sapiens
101 DENND1A 57706
Affinity Capture-MS Homo sapiens
102 PPM1H  
Affinity Capture-MS Homo sapiens
103 SRSF12  
Affinity Capture-MS Homo sapiens
104 Cep135  
Affinity Capture-MS Mus musculus
105 CAPZA2 830
Affinity Capture-MS Homo sapiens
106 LATS2 26524
Proximity Label-MS Homo sapiens
107 CDC25C  
Affinity Capture-MS Homo sapiens
108 C11orf52 91894
Proximity Label-MS Homo sapiens
109 Lrrcc1  
Affinity Capture-MS Mus musculus
110 NF1 4763
Affinity Capture-MS Homo sapiens
111 TRIM37  
Proximity Label-MS Homo sapiens
112 CEP135  
Proximity Label-MS Homo sapiens
113 FLNC 2318
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
114 EPHA2 1969
Proximity Label-MS Homo sapiens
115 GGA2 23062
Proximity Label-MS Homo sapiens
116 CUL7 9820
Affinity Capture-MS Homo sapiens
117 KRAS 3845
Synthetic Lethality Homo sapiens
Proximity Label-MS Homo sapiens
118 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
119 MAST3  
Affinity Capture-MS Homo sapiens
120 MAGI1  
Affinity Capture-MS Homo sapiens
121 GRIA2  
Affinity Capture-Western Homo sapiens
122 Smn1 20595
Affinity Capture-MS Mus musculus
123 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PHLDB2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here