Gene description for MAPKAP1
Gene name mitogen-activated protein kinase associated protein 1
Gene symbol MAPKAP1
Other names/aliases JC310
MIP1
SIN1
SIN1b
SIN1g
Species Homo sapiens
 Database cross references - MAPKAP1
ExoCarta ExoCarta_79109
Vesiclepedia VP_79109
Entrez Gene 79109
HGNC 18752
MIM 610558
UniProt Q9BPZ7  
 MAPKAP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAPKAP1
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IBA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    protein kinase binding GO:0019901 IPI
    small GTPase binding GO:0031267 IDA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    molecular adaptor activity GO:0060090 IDA
    phosphatidic acid binding GO:0070300 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
    enzyme-substrate adaptor activity GO:0140767 IDA
Biological Process
    cytoskeleton organization GO:0007010 NAS
    substantia nigra development GO:0021762 HEP
    positive regulation of cell growth GO:0030307 NAS
    cellular response to nutrient levels GO:0031669 NAS
    cellular response to insulin stimulus GO:0032869 ISS
    TORC2 signaling GO:0038203 IBA
    TORC2 signaling GO:0038203 IDA
    negative regulation of apoptotic process GO:0043066 NAS
    negative regulation of Ras protein signal transduction GO:0046580 IMP
    regulation of cellular response to oxidative stress GO:1900407 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrial outer membrane GO:0005741 IDA
    lysosome GO:0005764 IDA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    TORC2 complex GO:0031932 IBA
    TORC2 complex GO:0031932 IDA
    TORC2 complex GO:0031932 IDA
    TORC2 complex GO:0031932 IPI
 Experiment description of studies that identified MAPKAP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAPKAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC4  
Affinity Capture-MS Homo sapiens
2 KSR1  
Affinity Capture-MS Homo sapiens
3 NAV1 89796
Affinity Capture-MS Homo sapiens
4 BRCA1 672
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 CDC25B 994
Affinity Capture-MS Homo sapiens
6 SRGAP2 23380
Affinity Capture-MS Homo sapiens
7 ECI1 1632
Two-hybrid Homo sapiens
8 CAMSAP2  
Affinity Capture-MS Homo sapiens
9 CBY1  
Affinity Capture-MS Homo sapiens
10 PRR5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 EIF4E2  
Affinity Capture-MS Homo sapiens
12 KIF13B 23303
Affinity Capture-MS Homo sapiens
13 YWHAZ 7534
Affinity Capture-MS Homo sapiens
14 ZBTB21  
Affinity Capture-MS Homo sapiens
15 RASAL2 9462
Affinity Capture-MS Homo sapiens
16 ZNF638 27332
Affinity Capture-MS Homo sapiens
17 RAB3IP  
Affinity Capture-MS Homo sapiens
18 Cep170  
Affinity Capture-MS Mus musculus
19 MLST8 64223
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TIAM1  
Affinity Capture-MS Homo sapiens
22 PHLDB2 90102
Affinity Capture-MS Homo sapiens
23 YWHAH 7533
Affinity Capture-MS Homo sapiens
24 SFN 2810
Affinity Capture-MS Homo sapiens
25 LRFN1  
Affinity Capture-MS Homo sapiens
26 SNX27 81609
Affinity Capture-MS Homo sapiens
27 WDR5 11091
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
28 KIAA1804  
Affinity Capture-MS Homo sapiens
29 MELK  
Affinity Capture-MS Homo sapiens
30 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PRR5L  
Affinity Capture-Western Homo sapiens
32 CDK16 5127
Affinity Capture-MS Homo sapiens
33 ANKRD34A  
Affinity Capture-MS Homo sapiens
34 USP21  
Affinity Capture-MS Homo sapiens
35 FAM110A  
Affinity Capture-MS Homo sapiens
36 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
37 TESK2  
Affinity Capture-MS Homo sapiens
38 NBN 4683
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 DNAJC5 80331
Proximity Label-MS Homo sapiens
40 RPS6KB1 6198
Co-fractionation Homo sapiens
41 LPIN3 64900
Affinity Capture-MS Homo sapiens
42 FAM110B  
Affinity Capture-MS Homo sapiens
43 INPP5E 56623
Affinity Capture-MS Homo sapiens
44 MAPK8 5599
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
45 KCTD3  
Affinity Capture-MS Homo sapiens
46 TRIM52  
Affinity Capture-MS Homo sapiens
47 LIMA1 51474
Affinity Capture-MS Homo sapiens
48 DCLK1 9201
Affinity Capture-MS Homo sapiens
49 RPL23 9349
Affinity Capture-Western Homo sapiens
50 TP53BP1 7158
Negative Genetic Homo sapiens
51 XPO1 7514
Affinity Capture-MS Homo sapiens
52 CDC42EP1 11135
Two-hybrid Homo sapiens
53 CUL3 8452
Affinity Capture-MS Homo sapiens
54 RICTOR 253260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
55 NADK 65220
Affinity Capture-MS Homo sapiens
56 ABCB1 5243
Two-hybrid Homo sapiens
57 RIPK4  
Affinity Capture-MS Homo sapiens
58 SYDE1 85360
Affinity Capture-MS Homo sapiens
59 AURKB 9212
Affinity Capture-MS Homo sapiens
60 CDC25C  
Affinity Capture-MS Homo sapiens
61 KCNE3  
Affinity Capture-MS Homo sapiens
62 MAPK6  
Affinity Capture-MS Homo sapiens
63 GIGYF1  
Affinity Capture-MS Homo sapiens
64 MTOR 2475
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
65 CGN  
Affinity Capture-MS Homo sapiens
66 AKT1 207
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
67 TBC1D25  
Affinity Capture-MS Homo sapiens
68 DENND4C 55667
Affinity Capture-MS Homo sapiens
69 SH3RF3  
Affinity Capture-MS Homo sapiens
70 GIGYF2 26058
Affinity Capture-MS Homo sapiens
71 FASN 2194
Positive Genetic Homo sapiens
72 CTR9 9646
Affinity Capture-MS Homo sapiens
73 SGK1  
Reconstituted Complex Homo sapiens
74 FAM53C 51307
Affinity Capture-MS Homo sapiens
75 RTKN 6242
Affinity Capture-MS Homo sapiens
76 DENND1A 57706
Affinity Capture-MS Homo sapiens
77 STK38 11329
Affinity Capture-Western Homo sapiens
78 SRSF12  
Affinity Capture-MS Homo sapiens
79 PBRM1 55193
Negative Genetic Homo sapiens
80 GAB2 9846
Affinity Capture-MS Homo sapiens
81 AGAP1  
Affinity Capture-MS Homo sapiens
82 PPM1H  
Affinity Capture-MS Homo sapiens
83 NF1 4763
Affinity Capture-MS Homo sapiens
84 PRKCA 5578
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
85 PINK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
86 RPS6 6194
Affinity Capture-Western Homo sapiens
87 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
88 EPHA2 1969
Proximity Label-MS Homo sapiens
89 KRAS 3845
Proximity Label-MS Homo sapiens
90 CCDC96  
Affinity Capture-MS Homo sapiens
91 KIF1C 10749
Affinity Capture-MS Homo sapiens
92 MAST3  
Affinity Capture-MS Homo sapiens
93 MAGI1  
Affinity Capture-MS Homo sapiens
94 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
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