Gene description for ABCB1
Gene name ATP-binding cassette, sub-family B (MDR/TAP), member 1
Gene symbol ABCB1
Other names/aliases ABC20
CD243
CLCS
GP170
MDR1
P-GP
PGY1
Species Homo sapiens
 Database cross references - ABCB1
ExoCarta ExoCarta_5243
Vesiclepedia VP_5243
Entrez Gene 5243
HGNC 40
MIM 171050
UniProt P08183  
 ABCB1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Serum 25844599    
Serum 25844599    
Serum 25844599    
Serum 25844599    
Serum 25844599    
Serum 25844599    
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ABCB1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    ABC-type xenobiotic transporter activity GO:0008559 IDA
    ABC-type xenobiotic transporter activity GO:0008559 IGI
    ABC-type xenobiotic transporter activity GO:0008559 IMP
    ABC-type xenobiotic transporter activity GO:0008559 TAS
    efflux transmembrane transporter activity GO:0015562 IDA
    efflux transmembrane transporter activity GO:0015562 IGI
    efflux transmembrane transporter activity GO:0015562 IMP
    ATP hydrolysis activity GO:0016887 IEA
    transmembrane transporter activity GO:0022857 ISS
    ubiquitin protein ligase binding GO:0031625 IPI
    ATPase-coupled transmembrane transporter activity GO:0042626 IBA
    ATPase-coupled transmembrane transporter activity GO:0042626 IDA
    ATPase-coupled transmembrane transporter activity GO:0042626 TAS
    xenobiotic transmembrane transporter activity GO:0042910 IBA
    xenobiotic transmembrane transporter activity GO:0042910 IDA
    xenobiotic transmembrane transporter activity GO:0042910 IMP
    carboxylic acid transmembrane transporter activity GO:0046943 IMP
    phosphatidylcholine floppase activity GO:0090554 IBA
    phosphatidylcholine floppase activity GO:0090554 IDA
    phosphatidylethanolamine flippase activity GO:0090555 IDA
    ceramide floppase activity GO:0099038 IDA
    floppase activity GO:0140328 IMP
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 IDA
    xenobiotic metabolic process GO:0006805 TAS
    response to xenobiotic stimulus GO:0009410 TAS
    phospholipid translocation GO:0045332 IBA
    phospholipid translocation GO:0045332 IDA
    terpenoid transport GO:0046865 ISS
    transmembrane transport GO:0055085 TAS
    transepithelial transport GO:0070633 IDA
    transepithelial transport GO:0070633 IMP
    stem cell proliferation GO:0072089 IMP
    ceramide translocation GO:0099040 IBA
    ceramide translocation GO:0099040 IDA
    export across plasma membrane GO:0140115 IDA
    export across plasma membrane GO:0140115 IMP
    transport across blood-brain barrier GO:0150104 NAS
    carboxylic acid transmembrane transport GO:1905039 IMP
    xenobiotic detoxification by transmembrane export across the plasma membrane GO:1990961 IDA
    xenobiotic detoxification by transmembrane export across the plasma membrane GO:1990961 IGI
    xenobiotic detoxification by transmembrane export across the plasma membrane GO:1990961 IMP
    xenobiotic transport across blood-brain barrier GO:1990962 IC
    xenobiotic transport across blood-brain barrier GO:1990962 IMP
    xenobiotic transport across blood-brain barrier GO:1990962 ISS
    regulation of chloride transport GO:2001225 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    extracellular exosome GO:0070062 HDA
    external side of apical plasma membrane GO:0098591 ISS
 Experiment description of studies that identified ABCB1 in sEVs
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 274
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
10
Experiment ID 276
MISEV standards
Biophysical techniques
Alix|RAB5A|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Serum
Sample name Serum - Docetaxel resistant castration resistant prostate cancer patient 1
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 277
MISEV standards
Biophysical techniques
Alix|RAB5A|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Serum
Sample name Serum - Docetaxel resistant castration resistant prostate cancer patient 2
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 278
MISEV standards
Biophysical techniques
Alix|RAB5A|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Serum
Sample name Serum - Docetaxel resistant castration resistant prostate cancer patient 3
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 279
MISEV standards
Biophysical techniques
Alix|RAB5A
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Serum
Sample name Serum - Docetaxel sensitive castration resistant prostate cancer patient 4
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 280
MISEV standards
Biophysical techniques
Alix|RAB5A
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Serum
Sample name Serum - Docetaxel sensitive castration resistant prostate cancer patient 5
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
15
Experiment ID 281
MISEV standards
Biophysical techniques
Alix|RAB5A|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Serum
Sample name Serum - Docetaxel sensitive castration resistant prostate cancer patient 6
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
16
Experiment ID 13
MISEV standards
IEM
Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
17
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
18
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ABCB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TAS2R19  
Affinity Capture-MS Homo sapiens
2 ATPIF1 93974
Cross-Linking-MS (XL-MS) Homo sapiens
3 MAPKAP1 79109
Two-hybrid Homo sapiens
4 NEDD4 4734
Biochemical Activity Homo sapiens
5 TTYH1  
Affinity Capture-MS Homo sapiens
6 CANX 821
Affinity Capture-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 MARCH1  
Affinity Capture-MS Homo sapiens
9 ARRDC4  
Affinity Capture-MS Homo sapiens
10 ABCB5  
Cross-Linking-MS (XL-MS) Homo sapiens
11 SP1  
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
12 CD4 920
Two-hybrid Homo sapiens
13 MARCH8  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PIM1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
16 LGALS9B 284194
Affinity Capture-MS Homo sapiens
17 IFIT5 24138
Cross-Linking-MS (XL-MS) Homo sapiens
18 CEP250 11190
Cross-Linking-MS (XL-MS) Homo sapiens
19 PSMB8 5696
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 TMEM169  
Affinity Capture-MS Homo sapiens
21 MAPRE1 22919
Two-hybrid Homo sapiens
22 TBP  
Co-localization Homo sapiens
23 UBB 7314
Affinity Capture-Western Homo sapiens
24 SAAL1 113174
Affinity Capture-MS Homo sapiens
25 BCL2  
Affinity Capture-MS Homo sapiens
26 SP3  
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
27 NTRK1 4914
Affinity Capture-MS Homo sapiens
28 C3orf52  
Affinity Capture-MS Homo sapiens
29 RAB11A 8766
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 BCCIP 56647
Two-hybrid Homo sapiens
31 CUL7 9820
Affinity Capture-MS Homo sapiens
32 ABCB4 5244
Cross-Linking-MS (XL-MS) Homo sapiens
33 DHX9 1660
Affinity Capture-MS Homo sapiens
34 CCR6  
Affinity Capture-MS Homo sapiens
35 ABCB9  
Cross-Linking-MS (XL-MS) Homo sapiens
36 LNX1  
Two-hybrid Homo sapiens
37 EDAR  
Affinity Capture-MS Homo sapiens
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