Experiment description of studies that identified BCCIP in exosomes

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 191 MISEV standards ✘ Biophysical techniques ✔ Alix|CD81|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20124223     Organism Homo sapiens Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK." Journal name Mol Cell Proteomics Publication year 2010 Sample Squamous carcinoma cells Sample name Squamous carcinoma cell (A431) Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for BCCIP

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RALY 22913 Two-hybrid Homo sapiens 2 LDHA 3939 Co-fractionation Homo sapiens 3 HOOK1   Proximity Label-MS Homo sapiens 4 NOP9 161424 Affinity Capture-MS Homo sapiens 5 RIT1 6016 Negative Genetic Homo sapiens 6 SSB 6741 Co-fractionation Homo sapiens 7 CENPJ 55835 Two-hybrid Homo sapiens 8 RPL10 6134 Affinity Capture-MS Homo sapiens 9 CSE1L 1434 Co-fractionation Homo sapiens 10 LOC101060521 101060521 Co-fractionation Homo sapiens 11 DNAJB6 10049 Proximity Label-MS Homo sapiens 12 GSK3A 2931 Two-hybrid Homo sapiens 13 TCEA1 6917 Co-fractionation Homo sapiens 14 CAPZB 832 Two-hybrid Homo sapiens 15 PARK2   Affinity Capture-MS Homo sapiens 16 FTO 79068 Co-fractionation Homo sapiens 17 DDX24 57062 Co-fractionation Homo sapiens 18 GNS 2799 Co-fractionation Homo sapiens 19 SRC 6714 Biochemical Activity Homo sapiens 20 NPM1 4869 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 CRCP   Co-fractionation Homo sapiens 22 GRWD1 83743 Co-fractionation Homo sapiens 23 TULP3 7289 Affinity Capture-MS Homo sapiens 24 MARCKSL1 65108 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 KIF2C 11004 Affinity Capture-MS Homo sapiens 26 DDX18 8886 Co-fractionation Homo sapiens 27 NAGK 55577 Co-fractionation Homo sapiens 28 PAPD4 167153 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 CSNK2B 1460 Two-hybrid Homo sapiens 30 RPL19 6143 Affinity Capture-MS Homo sapiens 31 GSK3B 2932 Two-hybrid Homo sapiens 32 GDA 9615 Co-fractionation Homo sapiens 33 FCF1   Two-hybrid Homo sapiens 34 YWHAB 7529 Co-fractionation Homo sapiens Co-fractionation Homo sapiens 35 PEPD 5184 Co-fractionation Homo sapiens 36 LMNB1 4001 Co-fractionation Homo sapiens 37 EMD 2010 Affinity Capture-MS Homo sapiens 38 CDKN1A   Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 39 RPL4 6124 Affinity Capture-MS Homo sapiens 40 CCDC33 80125 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 41 BRCA2 675 Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 42 Srp72   Affinity Capture-MS Mus musculus 43 BARD1 580 Two-hybrid Homo sapiens 44 NTRK1 4914 Affinity Capture-MS Homo sapiens 45 BICD2 23299 Proximity Label-MS Homo sapiens 46 RPA4   Proximity Label-MS Homo sapiens 47 SMS 6611 Co-fractionation Homo sapiens 48 CAPN1 823 Co-fractionation Homo sapiens 49 ZNF138   Two-hybrid Homo sapiens 50 HMGB2 3148 Two-hybrid Homo sapiens 51 VCP 7415 Affinity Capture-MS Homo sapiens 52 PTN   Two-hybrid Homo sapiens 53 PTPRM 5797 Two-hybrid Homo sapiens Biochemical Activity Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 54 OGT 8473 Reconstituted Complex Homo sapiens 55 EIF6 3692 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 56 ZNF19   Two-hybrid Homo sapiens 57 FAM46B   Affinity Capture-MS Homo sapiens 58 PDIA3 2923 Co-fractionation Homo sapiens 59 EGFR 1956 Affinity Capture-MS Homo sapiens Negative Genetic Homo sapiens 60 YY1 7528 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 61 ABCB1 5243 Two-hybrid Homo sapiens 62 SORD 6652 Co-fractionation Homo sapiens 63 POLR3E 55718 Co-fractionation Homo sapiens 64 HOOK3 84376 Proximity Label-MS Homo sapiens 65 CDK2 1017 Affinity Capture-Western Homo sapiens 66 DKC1 1736 Co-fractionation Homo sapiens 67 RPA3 6119 Proximity Label-MS Homo sapiens 68 FAM46A   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 69 POLR1A   Co-fractionation Homo sapiens 70 BICD1 636 Proximity Label-MS Homo sapiens 71 DDRGK1 65992 Affinity Capture-MS Homo sapiens 72 Rpl35 66489 Affinity Capture-MS Mus musculus 73 Mad2l1 56150 Affinity Capture-MS Mus musculus 74 LMNA 4000 Proximity Label-MS Homo sapiens 75 GNL3 26354 Co-fractionation Homo sapiens 76 THOC1 9984 Affinity Capture-MS Homo sapiens 77 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 78 PSMB10 5699 Affinity Capture-MS Homo sapiens 79 LDHAL6B 92483 Co-fractionation Homo sapiens 80 BKRF1   Affinity Capture-MS 81 ALDH9A1 223 Co-fractionation Homo sapiens 82 ZRANB2 9406 Co-fractionation Homo sapiens 83 TP53 7157 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 84 POLR1C 9533 Co-fractionation Homo sapiens 85 Itsn2   Affinity Capture-MS Mus musculus 86 HIST1H2BN 8341 Two-hybrid Homo sapiens 87 ITSN2 50618 Two-hybrid Homo sapiens 88 RPL38 6169 Two-hybrid Homo sapiens 89 CACNG8   Affinity Capture-MS Homo sapiens 90 RPL23 9349 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 91 KYNU   Co-fractionation Homo sapiens 92 EIF5A 1984 Co-fractionation Homo sapiens 93 HNRNPU 3192 Affinity Capture-MS Homo sapiens 94 C9orf72   Affinity Capture-MS Homo sapiens 95 FHDC1 85462 Cross-Linking-MS (XL-MS) Homo sapiens 96 CHORDC1 26973 Co-fractionation Homo sapiens 97 MRPS9 64965 Two-hybrid Homo sapiens 98 EP300 2033 Affinity Capture-MS Homo sapiens 99 CAPN2 824 Co-fractionation Homo sapiens View the network

Pathways in which BCCIP is involved

No pathways found