Gene description for Mad2l1
Gene name MAD2 mitotic arrest deficient-like 1
Gene symbol Mad2l1
Other names/aliases AA673185
MAD2
Species Mus musculus
 Database cross references - Mad2l1
ExoCarta ExoCarta_56150
Vesiclepedia VP_56150
Entrez Gene 56150
UniProt Q9Z1B5  
 Mad2l1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mad2l1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
Biological Process
    mitotic sister chromatid segregation GO:0000070 IMP
    establishment of mitotic spindle orientation GO:0000132 IEA
    establishment of mitotic spindle orientation GO:0000132 ISO
    mitotic cell cycle checkpoint signaling GO:0007093 ISO
    mitotic spindle assembly checkpoint signaling GO:0007094 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 IMP
    mitotic spindle assembly checkpoint signaling GO:0007094 ISA
    mitotic spindle assembly checkpoint signaling GO:0007094 ISO
    mitotic spindle assembly checkpoint signaling GO:0007094 NAS
    negative regulation of protein catabolic process GO:0042177 ISO
    negative regulation of protein catabolic process GO:0042177 ISS
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of mitotic metaphase/anaphase transition GO:0045841 TAS
    negative regulation of mitotic cell cycle GO:0045930 ISO
    cell division GO:0051301 IEA
    establishment of centrosome localization GO:0051660 IEA
    establishment of centrosome localization GO:0051660 ISO
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 IMP
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 ISO
    negative regulation of ubiquitin protein ligase activity GO:1904667 ISO
Subcellular Localization
    chromosome, centromeric region GO:0000775 IDA
    kinetochore GO:0000776 IDA
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISO
    spindle pole GO:0000922 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    chromosome GO:0005694 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    mitotic checkpoint complex GO:0033597 ISO
    nuclear pore nuclear basket GO:0044615 IEA
    nuclear pore nuclear basket GO:0044615 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
    mitotic spindle assembly checkpoint MAD1-MAD2 complex GO:1990728 IPI
    mitotic spindle assembly checkpoint MAD1-MAD2 complex GO:1990728 ISO
 Experiment description of studies that identified Mad2l1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mad2l1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C15orf39  
Affinity Capture-MS Homo sapiens
2 PRIM2 5558
Affinity Capture-MS Homo sapiens
3 CDC26 246184
Affinity Capture-MS Homo sapiens
4 EPM2AIP1 9852
Affinity Capture-MS Homo sapiens
5 STT3A 3703
Affinity Capture-MS Homo sapiens
6 PFKP 5214
Affinity Capture-MS Homo sapiens
7 INIP  
Affinity Capture-MS Homo sapiens
8 ANAPC5 51433
Affinity Capture-MS Homo sapiens
9 JPH1 56704
Affinity Capture-MS Homo sapiens
10 GPC1 2817
Affinity Capture-MS Homo sapiens
11 CDC23 8697
Affinity Capture-MS Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MAD2L1BP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PMF1  
Affinity Capture-MS Homo sapiens
15 ATP5L 10632
Affinity Capture-MS Homo sapiens
16 Tmem173  
Proximity Label-MS Mus musculus
17 CDC16 8881
Affinity Capture-MS Homo sapiens
18 PFKL 5211
Affinity Capture-MS Homo sapiens
19 NPLOC4 55666
Affinity Capture-MS Homo sapiens
20 AHSG 197
Affinity Capture-MS Homo sapiens
21 TIMM23 100287932
Affinity Capture-MS Homo sapiens
22 PRIM1  
Affinity Capture-MS Homo sapiens
23 MGAT1 4245
Affinity Capture-MS Homo sapiens
24 BRPF3  
Affinity Capture-MS Homo sapiens
25 RAD51  
Affinity Capture-MS Homo sapiens
26 SLC25A19  
Affinity Capture-MS Homo sapiens
27 CDC27 996
Affinity Capture-MS Homo sapiens
28 MRPL39 54148
Affinity Capture-MS Homo sapiens
29 ANAPC7 51434
Affinity Capture-MS Homo sapiens
30 TCP11L1 55346
Affinity Capture-MS Homo sapiens
31 PALLD 23022
Affinity Capture-MS Homo sapiens
32 KNSTRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MAD2L1 4085
Affinity Capture-MS Homo sapiens
34 PSME3 10197
Affinity Capture-MS Homo sapiens
35 BSG 682
Affinity Capture-MS Homo sapiens
36 SPAG5 10615
Affinity Capture-MS Homo sapiens
37 Foxp3  
Affinity Capture-MS Mus musculus
38 RPP14  
Affinity Capture-MS Homo sapiens
39 EMD 2010
Affinity Capture-MS Homo sapiens
40 ANTXR1 84168
Affinity Capture-MS Homo sapiens
41 FAM96B 51647
Affinity Capture-MS Homo sapiens
42 CPS1 1373
Affinity Capture-MS Homo sapiens
43 POLA2  
Affinity Capture-MS Homo sapiens
44 SLC9A1 6548
Affinity Capture-MS Homo sapiens
45 DNAJA1 3301
Affinity Capture-MS Homo sapiens
46 MRPL3  
Affinity Capture-MS Homo sapiens
47 ANAPC4 29945
Affinity Capture-MS Homo sapiens
48 FERMT1 55612
Affinity Capture-MS Homo sapiens
49 PDF  
Affinity Capture-MS Homo sapiens
50 SP140L  
Affinity Capture-MS Homo sapiens
51 BCLAF1 9774
Affinity Capture-MS Homo sapiens
52 Ubc  
Reconstituted Complex Mus musculus
53 TPX2  
Affinity Capture-MS Homo sapiens
54 TUBB 203068
Affinity Capture-MS Homo sapiens
55 SPTLC1 10558
Affinity Capture-MS Homo sapiens
56 BUB1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 TPR 7175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 MAD1L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 C1orf52  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 FKBP8 23770
Affinity Capture-MS Homo sapiens
61 FLYWCH1  
Affinity Capture-MS Homo sapiens
62 ATXN10 25814
Affinity Capture-MS Homo sapiens
63 NAP1L1 4673
Affinity Capture-MS Homo sapiens
64 WIBG 84305
Affinity Capture-MS Homo sapiens
65 RCN1 5954
Affinity Capture-MS Homo sapiens
66 KLHL13 90293
Affinity Capture-MS Homo sapiens
67 FOXM1  
Affinity Capture-MS Homo sapiens
68 DMAP1 55929
Affinity Capture-MS Homo sapiens
69 ATP5E 514
Affinity Capture-MS Homo sapiens
70 PKP4 8502
Affinity Capture-MS Homo sapiens
71 TRIM27  
Affinity Capture-MS Homo sapiens
72 ZFP91 80829
Affinity Capture-MS Homo sapiens
73 Eed  
Affinity Capture-MS Mus musculus
74 MRPL38  
Affinity Capture-MS Homo sapiens
75 ARAF 369
Affinity Capture-MS Homo sapiens
76 GSN 2934
Affinity Capture-MS Homo sapiens
77 LBR 3930
Affinity Capture-MS Homo sapiens
78 DIS3L2 129563
Affinity Capture-MS Homo sapiens
79 SLC25A11 8402
Affinity Capture-MS Homo sapiens
80 CDC20 991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 MRPS2 51116
Affinity Capture-MS Homo sapiens
82 UTP6  
Affinity Capture-MS Homo sapiens
83 TUBG1 7283
Affinity Capture-MS Homo sapiens
84 CDCA7L  
Affinity Capture-MS Homo sapiens
85 Atxn1  
Affinity Capture-MS Mus musculus
86 TUBB3 10381
Affinity Capture-MS Homo sapiens
87 THRAP3 9967
Affinity Capture-MS Homo sapiens
88 INSR 3643
Affinity Capture-MS Homo sapiens
89 ANAPC1 64682
Affinity Capture-MS Homo sapiens
90 MLH1 4292
Affinity Capture-MS Homo sapiens
91 EBP  
Affinity Capture-MS Homo sapiens
92 Iqcb1  
Affinity Capture-MS Mus musculus
93 TOR1AIP1 26092
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 PRR12 57479
Affinity Capture-MS Homo sapiens
95 HMGXB4 10042
Affinity Capture-MS Homo sapiens
96 IDH2 3418
Affinity Capture-MS Homo sapiens
97 DAPK3 1613
Affinity Capture-MS Homo sapiens
98 BCCIP 56647
Affinity Capture-MS Homo sapiens
99 CEP78 84131
Affinity Capture-MS Homo sapiens
100 ITPR1 3708
Affinity Capture-MS Homo sapiens
101 POLA1  
Affinity Capture-MS Homo sapiens
102 STT3B 201595
Affinity Capture-MS Homo sapiens
103 CYFIP1 23191
Affinity Capture-MS Homo sapiens
104 PSMD3 5709
Affinity Capture-MS Homo sapiens
105 NUDT5 11164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 PVRL3 25945
Affinity Capture-MS Homo sapiens
107 RANBP2 5903
Affinity Capture-MS Homo sapiens
108 SEC61A1 29927
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Mad2l1 is involved
PathwayEvidenceSource
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
APC-Cdc20 mediated degradation of Nek2A IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
Inactivation of APC/C via direct inhibition of the APC/C complex IEA Reactome
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components IEA Reactome
M Phase IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Regulation of APC/C activators between G1/S and early anaphase IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome





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