Gene description for DAPK3
Gene name death-associated protein kinase 3
Gene symbol DAPK3
Other names/aliases DLK
ZIP
ZIPK
Species Homo sapiens
 Database cross references - DAPK3
ExoCarta ExoCarta_1613
Vesiclepedia VP_1613
Entrez Gene 1613
HGNC 2676
MIM 603289
UniProt O43293  
 DAPK3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DAPK3
Molecular Function
    identical protein binding GO:0042802 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
    ATP binding GO:0005524 IDA
    calmodulin-dependent protein kinase activity GO:0004683 IBA
    protein binding GO:0005515 IPI
    leucine zipper domain binding GO:0043522 IPI
    cAMP response element binding protein binding GO:0008140 TAS
    Rho GTPase binding GO:0017048 IDA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    protein phosphorylation GO:0006468 IDA
    regulation of cell shape GO:0008360 IEA
    regulation of transcription, DNA-templated GO:0006355 TAS
    positive regulation of apoptotic process GO:0043065 IDA
    apoptotic process GO:0006915 IMP
    regulation of cell motility GO:2000145 TAS
    positive regulation of cell migration GO:0030335 IEA
    regulation of mitotic nuclear division GO:0007088 TAS
    regulation of smooth muscle contraction GO:0006940 TAS
    cellular response to interferon-gamma GO:0071346 IDA
    regulation of actin cytoskeleton reorganization GO:2000249 TAS
    apoptotic signaling pathway GO:0097190 IEA
    chromatin modification GO:0016568 IEA
    regulation of autophagy GO:0010506 TAS
    transcription, DNA-templated GO:0006351 IEA
    intracellular signal transduction GO:0035556 IDA
    negative regulation of translation GO:0017148 IDA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    protein autophosphorylation GO:0046777 TAS
    neuron differentiation GO:0030182 IEA
    regulation of focal adhesion assembly GO:0051893 IDA
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 IEA
    cytokinesis GO:0000910 TAS
    regulation of myosin II filament organization GO:0043519 IEA
    regulation of mitotic cell cycle GO:0007346 IMP
    regulation of apoptotic process GO:0042981 TAS
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IEA
    membrane raft GO:0045121 IEA
    PML body GO:0016605 IEA
 Experiment description of studies that identified DAPK3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DAPK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRB14 2888
Reconstituted Complex Homo sapiens
2 Atf4  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 PRKCZ 5590
Reconstituted Complex Homo sapiens
4 AATF  
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 PAWR 5074
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
6 DAXX  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which DAPK3 is involved
PathwayEvidenceSource
Ligand-independent caspase activation via DCC TAS Reactome





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