Gene description for Flot2
Gene name flotillin 2
Gene symbol Flot2
Other names/aliases AI573412
Esa
reggie-2
Species Mus musculus
 Database cross references - Flot2
ExoCarta ExoCarta_14252
Vesiclepedia VP_14252
Entrez Gene 14252
UniProt Q60634  
 Flot2 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 19415654    
Colon cancer cells 37309723    
Motor neuron-like cells 36214496    
Motor neuron-like cells 36214496    
Oligodendrocytes 18309083    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Flot2
Molecular Function
    protease binding GO:0002020 IBA
    protease binding GO:0002020 ISO
    ionotropic glutamate receptor binding GO:0035255 ISO
Biological Process
    cell adhesion GO:0007155 IEA
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    protein localization to plasma membrane raft GO:0044860 IEA
    protein localization to plasma membrane raft GO:0044860 ISO
    regulation of myoblast differentiation GO:0045661 IBA
    regulation of myoblast differentiation GO:0045661 IEP
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    anterograde dendritic transport GO:0098937 IDA
    anterograde dendritic transport GO:0098937 IMP
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IDA
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IMP
    negative regulation of amyloid precursor protein catabolic process GO:1902992 IEA
    negative regulation of amyloid precursor protein catabolic process GO:1902992 ISO
    positive regulation of establishment of T cell polarity GO:1903905 IEA
    positive regulation of establishment of T cell polarity GO:1903905 ISO
Subcellular Localization
    uropod GO:0001931 IEA
    uropod GO:0001931 ISO
    acrosomal membrane GO:0002080 IDA
    endosome GO:0005768 ISO
    endosome GO:0005768 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    caveola GO:0005901 IDA
    caveola GO:0005901 ISO
    adherens junction GO:0005912 IEA
    adherens junction GO:0005912 ISO
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    flotillin complex GO:0016600 IBA
    flotillin complex GO:0016600 IDA
    flotillin complex GO:0016600 ISO
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    endocytic vesicle GO:0030139 ISO
    endocytic vesicle GO:0030139 ISS
    cortical actin cytoskeleton GO:0030864 IEA
    cortical actin cytoskeleton GO:0030864 ISO
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 IDA
    cytoplasmic vesicle GO:0031410 ISO
    vesicle GO:0031982 ISO
    dendrite cytoplasm GO:0032839 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    cell-cell contact zone GO:0044291 IEA
    cell-cell contact zone GO:0044291 ISO
    membrane raft GO:0045121 IDA
    synapse GO:0045202 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    presynaptic active zone membrane GO:0048787 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Flot2 in sEVs
1
Experiment ID 65
MISEV standards
✘ Biophysical techniques
✔
HSP60|HSP27|GAPDH|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors "Staubach S, Razawi H, Hanisch FG."
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 505
MISEV standards
✘ Biophysical techniques
✔
Flot2
Enriched markers
✔
Tomm20
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36214496    
Organism Mus musculus
Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA
Authors "McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ."
Journal name J Extracell Vesicles
Publication year 2022
Sample Motor neuron-like cells
Sample name NSC-34
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 506
MISEV standards
✘ Biophysical techniques
✔
Flot2
Enriched markers
✔
Tomm20
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36214496    
Organism Mus musculus
Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA
Authors "McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ."
Journal name J Extracell Vesicles
Publication year 2022
Sample Motor neuron-like cells
Sample name NSC-34
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 130
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|FLOT2
Enriched markers
✔
CANX|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 18309083    
Organism Mus musculus
Experiment description Ceramide triggers budding of exosome vesicles into multivesicular endosomes
Authors "Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, Schwille P, Brugger B, Simons M."
Journal name SCIENCE
Publication year 2008
Sample Oligodendrocytes
Sample name Oli-neu cells
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry [QTOF]
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Flot2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRN 286262
Affinity Capture-MS Homo sapiens
2 NDUFV3 4731
Affinity Capture-MS Homo sapiens
3 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
4 CYBRD1 79901
Affinity Capture-MS Homo sapiens
5 LIMA1 51474
Affinity Capture-MS Homo sapiens
6 NEXN 91624
Affinity Capture-MS Homo sapiens
7 ABLIM1 3983
Affinity Capture-MS Homo sapiens
8 SPTAN1 6709
Affinity Capture-MS Homo sapiens
9 ZDHHC5 25921
Affinity Capture-MS Homo sapiens
10 TWF1 5756
Affinity Capture-MS Homo sapiens
11 SVIL 6840
Affinity Capture-MS Homo sapiens
12 ARPC5L 81873
Affinity Capture-MS Homo sapiens
13 SPECC1 92521
Affinity Capture-MS Homo sapiens
14 PTRF 284119
Affinity Capture-MS Homo sapiens
15 COBL  
Affinity Capture-MS Homo sapiens
16 TPM2 7169
Affinity Capture-MS Homo sapiens
17 LMO7 4008
Affinity Capture-MS Homo sapiens
18 MISP 126353
Affinity Capture-MS Homo sapiens
19 SETX  
Affinity Capture-MS Homo sapiens
20 APC  
Affinity Capture-MS Homo sapiens
21 DBN1 1627
Affinity Capture-MS Homo sapiens
22 SIPA1L3  
Affinity Capture-MS Homo sapiens
23 Kctd13  
Affinity Capture-MS Mus musculus
24 Tfe3  
Affinity Capture-MS Mus musculus
25 CTTN 2017
Affinity Capture-MS Homo sapiens
26 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
27 BASP1 10409
Affinity Capture-MS Homo sapiens
28 LUZP1 7798
Affinity Capture-MS Homo sapiens
29 PACSIN3 29763
Affinity Capture-MS Homo sapiens
30 DIXDC1  
Affinity Capture-MS Homo sapiens
31 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
32 CORO1C 23603
Affinity Capture-MS Homo sapiens
33 TMOD1  
Affinity Capture-MS Homo sapiens
34 SPTBN1 6711
Affinity Capture-MS Homo sapiens
35 Tmem173  
Proximity Label-MS Mus musculus
36 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
37 AFAP1 60312
Affinity Capture-MS Homo sapiens
38 SYNPO 11346
Affinity Capture-MS Homo sapiens
39 GNAS 2778
Affinity Capture-MS Homo sapiens
40 TMEM237 65062
Affinity Capture-MS Homo sapiens
41 INF2 64423
Affinity Capture-MS Homo sapiens
42 PRKCDBP 112464
Affinity Capture-MS Homo sapiens
43 ACTN4 81
Affinity Capture-MS Homo sapiens
44 DAPK3 1613
Affinity Capture-MS Homo sapiens
45 FLNA 2316
Affinity Capture-MS Homo sapiens
46 ARHGAP11A  
Affinity Capture-MS Homo sapiens
47 RAB11FIP2  
Affinity Capture-MS Homo sapiens
48 CGN  
Affinity Capture-MS Homo sapiens
49 FLII 2314
Affinity Capture-MS Homo sapiens
50 AKAP2 11217
Affinity Capture-MS Homo sapiens
51 Eed  
Affinity Capture-MS Mus musculus
52 ANXA2 302
Affinity Capture-MS Homo sapiens
53 MIS18BP1  
Affinity Capture-MS Homo sapiens
54 KIDINS220 57498
Affinity Capture-MS Homo sapiens
55 CAV1 857
Affinity Capture-MS Homo sapiens
56 ACTG1 71
Affinity Capture-MS Homo sapiens
57 FLOT1 10211
Affinity Capture-MS Homo sapiens
58 FARP1 10160
Affinity Capture-MS Homo sapiens
59 DSG2 1829
Affinity Capture-MS Homo sapiens
60 GPRC5A 9052
Affinity Capture-MS Homo sapiens
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