Gene description for AFAP1
Gene name actin filament associated protein 1
Gene symbol AFAP1
Other names/aliases AFAP
AFAP-110
AFAP110
Species Homo sapiens
 Database cross references - AFAP1
ExoCarta ExoCarta_60312
Entrez Gene 60312
HGNC 24017
MIM 608252
UniProt Q8N556  
 AFAP1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for AFAP1
Molecular Function
    actin binding GO:0003779 IEA
Biological Process
    regulation of cytoskeleton organization GO:0051493 IEA
    regulation of signal transduction GO:0009966 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    actin cytoskeleton GO:0015629 IDA
    focal adhesion GO:0005925 IDA
 Experiment description of studies that identified AFAP1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for AFAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKCG 5582
Invitro Homo sapiens
2 SRC 6714
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
3 ACTC1 70
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
4 PRKCZ 5590
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
5 AFAP1 60312
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
6 PRKCB 5579
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
7 PRKCE  
Invitro Homo sapiens
8 PRKCD 5580
Invitro Homo sapiens
9 PRKCA 5578
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which AFAP1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here