Experiment description of studies that identified AFAP1 in exosomes

1 Experiment ID 235 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for AFAP1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PRKCG 5582 Invitro Homo sapiens 2 SRC 6714 Affinity Capture-MS Homo sapiens Invitro Homo sapiens 3 ACTC1 70 Affinity Capture-MS Homo sapiens Invivo Homo sapiens 4 PRKCZ 5590 Affinity Capture-MS Homo sapiens Invitro Homo sapiens 5 AFAP1 60312 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 6 PRKCB 5579 Affinity Capture-MS Homo sapiens Invitro Homo sapiens 7 PRKCE   Invitro Homo sapiens 8 PRKCD 5580 Invitro Homo sapiens 9 PRKCA 5578 Invivo Homo sapiens Invitro Homo sapiens View the network

Pathways in which AFAP1 is involved

No pathways found