Gene description for EXT2
Gene name exostosin glycosyltransferase 2
Gene symbol EXT2
Other names/aliases SOTV
Species Homo sapiens
 Database cross references - EXT2
ExoCarta ExoCarta_2132
Vesiclepedia VP_2132
Entrez Gene 2132
HGNC 3513
MIM 608210
UniProt Q93063  
 EXT2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Saliva 19199708    
 Gene ontology annotations for EXT2
Molecular Function
    protein binding GO:0005515 IPI
    acetylglucosaminyltransferase activity GO:0008375 IDA
    glucuronosyltransferase activity GO:0015020 IDA
    glycosyltransferase activity GO:0016757 IBA
    glycosyltransferase activity GO:0016757 IDA
    heparan sulfate N-acetylglucosaminyltransferase activity GO:0042328 NAS
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 IPI
    glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity GO:0050508 IDA
    N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity GO:0050509 IDA
    N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity GO:0050509 NAS
Biological Process
    polysaccharide biosynthetic process GO:0000271 IDA
    ossification GO:0001503 IMP
    mesoderm formation GO:0001707 IEA
    chondrocyte differentiation GO:0002062 IEA
    glycosaminoglycan biosynthetic process GO:0006024 IDA
    protein N-linked glycosylation GO:0006487 IEA
    regulation of blood pressure GO:0008217 IEA
    gene expression GO:0010467 IEA
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IMP
    heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process GO:0015014 IDA
    heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process GO:0015014 IMP
    heparin biosynthetic process GO:0030210 IEA
    fluid transport GO:0042044 IEA
    vasodilation GO:0042311 IEA
    cellular response to fibroblast growth factor stimulus GO:0044344 IEA
    multicellular organismal-level water homeostasis GO:0050891 IEA
    sulfation GO:0051923 IEA
    sodium ion homeostasis GO:0055078 IEA
    heart contraction GO:0060047 IEA
    endochondral bone morphogenesis GO:0060350 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISS
    membrane GO:0016020 HDA
    UDP-N-acetylglucosamine transferase complex GO:0043541 IDA
    extracellular exosome GO:0070062 HDA
    catalytic complex GO:1902494 IDA
 Experiment description of studies that identified EXT2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for EXT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 DNAJB9 4189
Affinity Capture-MS Homo sapiens
3 ADAM30 11085
Affinity Capture-MS Homo sapiens
4 GPIHBP1  
Affinity Capture-MS Homo sapiens
5 PRG2  
Affinity Capture-MS Homo sapiens
6 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
7 BTNL2  
Affinity Capture-MS Homo sapiens
8 TMEM106A  
Affinity Capture-MS Homo sapiens
9 STK16 8576
Affinity Capture-MS Homo sapiens
10 KLK15  
Affinity Capture-MS Homo sapiens
11 SMN1 6606
Two-hybrid Homo sapiens
12 TCEB1 6921
Affinity Capture-MS Homo sapiens
13 TK1 7083
Two-hybrid Homo sapiens
14 CANX 821
Affinity Capture-MS Homo sapiens
15 SLC22A2 6582
Two-hybrid Homo sapiens
16 DNAJB12 54788
Proximity Label-MS Homo sapiens
17 TRAP1 10131
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
18 FABP4 2167
Affinity Capture-MS Homo sapiens
19 ATE1 11101
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HPN  
Affinity Capture-MS Homo sapiens
21 PI15  
Affinity Capture-MS Homo sapiens
22 GGH 8836
Affinity Capture-MS Homo sapiens
23 CUX1 1523
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ANXA7 310
Two-hybrid Homo sapiens
25 CFC1  
Affinity Capture-MS Homo sapiens
26 GALNT5 11227
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
27 CLEC2L  
Affinity Capture-MS Homo sapiens
28 INVS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PFDN1 5201
Two-hybrid Homo sapiens
30 ECEL1  
Affinity Capture-MS Homo sapiens
31 EXTL3 2137
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DNAJC25 548645
Proximity Label-MS Homo sapiens
33 GSKIP  
Two-hybrid Homo sapiens
34 CDKN1A  
Two-hybrid Homo sapiens
35 LYZL1  
Affinity Capture-MS Homo sapiens
36 NOP9 161424
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NCR3  
Affinity Capture-MS Homo sapiens
38 AFAP1 60312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 KLK2  
Affinity Capture-MS Homo sapiens
40 SFTPC  
Affinity Capture-MS Homo sapiens
41 HNF4A  
Affinity Capture-MS Homo sapiens
42 USP30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 GAA 2548
Affinity Capture-MS Homo sapiens
44 CRB1  
Affinity Capture-MS Homo sapiens
45 EXT1 2131
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TTC19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SIRPD  
Affinity Capture-MS Homo sapiens
48 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 EXT2 2132
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 SDF2L1 23753
Affinity Capture-MS Homo sapiens
51 UGT1A7 54577
Affinity Capture-MS Homo sapiens
52 SCGB2A2  
Affinity Capture-MS Homo sapiens
53 PSCA 8000
Affinity Capture-MS Homo sapiens
54 PARK2  
Affinity Capture-MS Homo sapiens
55 DHH  
Affinity Capture-MS Homo sapiens
56 HYOU1 10525
Affinity Capture-MS Homo sapiens
57 GXYLT1 283464
Affinity Capture-MS Homo sapiens
58 CLEC2B  
Affinity Capture-MS Homo sapiens
59 CNTF  
Affinity Capture-MS Homo sapiens
60 EDN3  
Affinity Capture-MS Homo sapiens
61 MPPE1 65258
Affinity Capture-MS Homo sapiens
62 PLD3 23646
Affinity Capture-MS Homo sapiens
63 RPS29 6235
Affinity Capture-MS Homo sapiens
64 RBFA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SNCG 6623
Affinity Capture-MS Homo sapiens
66 TMPRSS13  
Affinity Capture-MS Homo sapiens
67 CD1A 909
Affinity Capture-MS Homo sapiens
68 HLA-C 3107
Affinity Capture-MS Homo sapiens
69 PRG3  
Affinity Capture-MS Homo sapiens
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