Gene description for HLA-DPB1
Gene name major histocompatibility complex, class II, DP beta 1
Gene symbol HLA-DPB1
Other names/aliases DPB1
HLA-DP
HLA-DP1B
HLA-DPB
Species Homo sapiens
 Database cross references - HLA-DPB1
ExoCarta ExoCarta_3115
Vesiclepedia VP_3115
Entrez Gene 3115
HGNC 4940
MIM 142858
UniProt P04440  
 HLA-DPB1 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for HLA-DPB1
Molecular Function
    protein binding GO:0005515 IPI
    MHC class II protein complex binding GO:0023026 IBA
    peptide antigen binding GO:0042605 IBA
    peptide antigen binding GO:0042605 IDA
Biological Process
    adaptive immune response GO:0002250 IEA
    peptide antigen assembly with MHC class II protein complex GO:0002503 IBA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IBA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IDA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IMP
    positive regulation of type II interferon production GO:0032729 IMP
    positive regulation of T cell proliferation GO:0042102 IMP
    positive regulation of immune response GO:0050778 IBA
    T cell receptor signaling pathway GO:0050852 IDA
    positive regulation of T cell activation GO:0050870 IBA
    positive regulation of T cell activation GO:0050870 IMP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 TAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IMP
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    membrane GO:0016020 HDA
    transport vesicle membrane GO:0030658 TAS
    endocytic vesicle membrane GO:0030666 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    late endosome membrane GO:0031902 IBA
    trans-Golgi network membrane GO:0032588 TAS
    MHC class II protein complex GO:0042613 IBA
    MHC class II protein complex GO:0042613 IDA
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
 Experiment description of studies that identified HLA-DPB1 in exosomes
1
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HLA-DPB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 HFE  
Affinity Capture-MS Homo sapiens
3 ZMYND19  
Affinity Capture-MS Homo sapiens
4 LGR4 55366
Affinity Capture-MS Homo sapiens
5 SCNN1A  
Affinity Capture-MS Homo sapiens
6 CACNA2D2 9254
Affinity Capture-MS Homo sapiens
7 HLA-E 3133
Affinity Capture-MS Homo sapiens
8 NUP188 23511
Affinity Capture-MS Homo sapiens
9 STIM2 57620
Affinity Capture-MS Homo sapiens
10 TUBB8 347688
Affinity Capture-MS Homo sapiens
11 GID4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 WDR26 80232
Affinity Capture-MS Homo sapiens
13 RTN4 57142
Two-hybrid Homo sapiens
14 CHST10  
Affinity Capture-MS Homo sapiens
15 VSTM2A  
Affinity Capture-MS Homo sapiens
16 RANBP10 57610
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TIMMDC1  
Affinity Capture-MS Homo sapiens
18 AHSG 197
Affinity Capture-MS Homo sapiens
19 SOST  
Affinity Capture-MS Homo sapiens
20 MKLN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 C2CD2 25966
Affinity Capture-MS Homo sapiens
22 PSG8  
Affinity Capture-MS Homo sapiens
23 PYCRL 65263
Affinity Capture-MS Homo sapiens
24 ACSL1 2180
Affinity Capture-MS Homo sapiens
25 TCP11L1 55346
Affinity Capture-MS Homo sapiens
26 HLA-G 3135
Affinity Capture-MS Homo sapiens
27 CTSV 1515
Affinity Capture-MS Homo sapiens
28 TMEM106C 79022
Affinity Capture-MS Homo sapiens
29 NOMO1 23420
Affinity Capture-MS Homo sapiens
30 ATP1B4  
Affinity Capture-MS Homo sapiens
31 TBC1D9B 23061
Affinity Capture-MS Homo sapiens
32 CD74 972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CEACAM21  
Affinity Capture-MS Homo sapiens
34 ARMC8 25852
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 YPEL5 51646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 NOL11  
Affinity Capture-MS Homo sapiens
37 COPB1 1315
Two-hybrid Homo sapiens
38 CNNM3 26505
Affinity Capture-MS Homo sapiens
39 GID8 54994
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SARAF  
Affinity Capture-MS Homo sapiens
41 ERP29 10961
Affinity Capture-MS Homo sapiens
42 MEOX2  
Two-hybrid Homo sapiens
43 LYRM4 57128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ANKRD13A 88455
Affinity Capture-MS Homo sapiens
45 RMND5A 64795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 FSTL1 11167
Affinity Capture-MS Homo sapiens
47 UBR2 23304
Affinity Capture-MS Homo sapiens
48 SERPINA6  
Affinity Capture-MS Homo sapiens
49 TMEM106B 54664
Affinity Capture-MS Homo sapiens
50 MSH2 4436
Affinity Capture-MS Homo sapiens
51 NFS1 9054
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 TMEM160 54958
Affinity Capture-MS Homo sapiens
53 ECE1 1889
Affinity Capture-MS Homo sapiens
54 GCNT2 2651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 RANBP9 10048
Affinity Capture-MS Homo sapiens
56 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
57 UBR1 197131
Affinity Capture-MS Homo sapiens
58 RARRES2 5919
Affinity Capture-MS Homo sapiens
59 KNG1 3827
Affinity Capture-MS Homo sapiens
60 CLPSL1  
Affinity Capture-MS Homo sapiens
61 TNFSF13B  
Affinity Capture-MS Homo sapiens
62 CBWD1 55871
Affinity Capture-MS Homo sapiens
63 GBAS 2631
Affinity Capture-MS Homo sapiens
64 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 ST3GAL5  
Affinity Capture-MS Homo sapiens
66 VWCE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 TRIP6 7205
Two-hybrid Homo sapiens
68 SPCS3 60559
Affinity Capture-MS Homo sapiens
69 SLC25A6 293
Affinity Capture-MS Homo sapiens
70 ATP1A3 478
Affinity Capture-MS Homo sapiens
71 HECTD3 79654
Affinity Capture-MS Homo sapiens
72 MISP 126353
Affinity Capture-MS Homo sapiens
73 PLXNA1 5361
Affinity Capture-MS Homo sapiens
74 ZZEF1 23140
Affinity Capture-MS Homo sapiens
75 SQSTM1 8878
Affinity Capture-MS Homo sapiens
76 LZTS2 84445
Two-hybrid Homo sapiens
77 SSBP2  
Affinity Capture-MS Homo sapiens
78 SFTPC  
Affinity Capture-MS Homo sapiens
79 IDE 3416
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 TAPBP 6892
Affinity Capture-MS Homo sapiens
81 EXT2 2132
Affinity Capture-MS Homo sapiens
82 SLC25A4 291
Affinity Capture-MS Homo sapiens
83 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 ROBO2 6092
Two-hybrid Homo sapiens
85 SMPD2 6610
Affinity Capture-MS Homo sapiens
86 PGM3 5238
Affinity Capture-MS Homo sapiens
87 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
88 FKBP2 2286
Affinity Capture-MS Homo sapiens
89 BRI3BP 140707
Affinity Capture-MS Homo sapiens
90 MAEA 10296
Affinity Capture-MS Homo sapiens
91 KIAA0922  
Affinity Capture-MS Homo sapiens
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