Gene description for ZZEF1
Gene name zinc finger, ZZ-type with EF-hand domain 1
Gene symbol ZZEF1
Other names/aliases ZZZ4
Species Homo sapiens
 Database cross references - ZZEF1
ExoCarta ExoCarta_23140
Vesiclepedia VP_23140
Entrez Gene 23140
HGNC 29027
UniProt O43149  
 ZZEF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ZZEF1
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    methylated histone binding GO:0035064 IDA
    lysine-acetylated histone binding GO:0070577 IDA
    histone reader activity GO:0140566 IMP
Biological Process
    chromatin organization GO:0006325 IEA
 Experiment description of studies that identified ZZEF1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ZZEF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vrk1  
Affinity Capture-MS Mus musculus
2 Tmed2 56334
Affinity Capture-MS Mus musculus
3 GMNN  
Affinity Capture-MS Homo sapiens
4 CLEC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RPS4Y1 6192
Affinity Capture-MS Homo sapiens
6 MRPL30  
Affinity Capture-MS Homo sapiens
7 TGS1  
Affinity Capture-MS Homo sapiens
8 MRPL10 124995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MRPL27 51264
Affinity Capture-MS Homo sapiens
10 LAD1 3898
Affinity Capture-MS Homo sapiens
11 CCDC9  
Co-fractionation Homo sapiens
12 GAN 8139
Affinity Capture-MS Homo sapiens
13 RSPO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 S100A4 6275
Co-fractionation Homo sapiens
15 PHYHIP 9796
Two-hybrid Homo sapiens
16 SULF2 55959
Affinity Capture-MS Homo sapiens
17 GABBR1  
Affinity Capture-MS Homo sapiens
18 PTPN4  
Two-hybrid Homo sapiens
19 PRC1 9055
Affinity Capture-MS Homo sapiens
20 MRPS24 64951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 WNT9B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 GCG  
Affinity Capture-MS Homo sapiens
23 SERPINA5 5104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 BAGE2  
Affinity Capture-MS Homo sapiens
25 FTSJ2 29960
Affinity Capture-MS Homo sapiens
26 MRPS26 64949
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 EPS15L1 58513
Co-fractionation Homo sapiens
28 IGF2 3481
Affinity Capture-MS Homo sapiens
29 ICAM4  
Affinity Capture-MS Homo sapiens
30 DEFB125  
Affinity Capture-MS Homo sapiens
31 MRPS7 51081
Affinity Capture-MS Homo sapiens
32 CCDC130  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 LRRC17 10234
Affinity Capture-MS Homo sapiens
34 THOC1 9984
Affinity Capture-MS Homo sapiens
35 C9orf163  
Affinity Capture-MS Homo sapiens
36 Tmed10 68581
Affinity Capture-MS Mus musculus
37 GPR156  
Affinity Capture-MS Homo sapiens
38 SPINK14  
Affinity Capture-MS Homo sapiens
39 AURKA 6790
Affinity Capture-MS Homo sapiens
40 TCOF1 6949
Co-fractionation Homo sapiens
41 C6orf203  
Proximity Label-MS Homo sapiens
42 TOP3B 8940
Affinity Capture-MS Homo sapiens
43 MRPS34 65993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PDGFRB 5159
Affinity Capture-MS Homo sapiens
45 IL25  
Affinity Capture-MS Homo sapiens
46 DCAF12 25853
Affinity Capture-MS Homo sapiens
47 DHRS4L2  
Affinity Capture-MS Homo sapiens
48 EMILIN3  
Affinity Capture-MS Homo sapiens
49 SCCPDH 51097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 HGF 3082
Affinity Capture-MS Homo sapiens
51 FEZF1  
Affinity Capture-MS Homo sapiens
52 PATE1  
Affinity Capture-MS Homo sapiens
53 GSG2  
Affinity Capture-MS Homo sapiens
54 NKAP 79576
Affinity Capture-MS Homo sapiens
55 COPS7B 64708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 HSPB3  
Two-hybrid Homo sapiens
57 TTC25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 DKK2  
Affinity Capture-MS Homo sapiens
59 EBI3 10148
Affinity Capture-MS Homo sapiens
60 CGGBP1 8545
Affinity Capture-MS Homo sapiens
61 ZRANB1 54764
Affinity Capture-MS Homo sapiens
62 PRSS33 260429
Affinity Capture-MS Homo sapiens
63 MRM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
65 ZBBX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ACSBG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
68 TRMT10C 54931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 CPA6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 LMNB1 4001
Co-fractionation Homo sapiens
71 MAD1L1  
Co-fractionation Homo sapiens
72 CDK5RAP1  
Affinity Capture-MS Homo sapiens
73 GAST  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 RPL3 6122
Affinity Capture-MS Homo sapiens
75 CUL3 8452
Affinity Capture-MS Homo sapiens
76 B4galt1  
Affinity Capture-MS Mus musculus
77 PRR27 401137
Affinity Capture-MS Homo sapiens
78 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 FOSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 AURKB 9212
Affinity Capture-MS Homo sapiens
81 GLIPR1L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 GADD45GIP1  
Affinity Capture-MS Homo sapiens
83 NCAPH 23397
Co-fractionation Homo sapiens
84 CNR2  
Affinity Capture-MS Homo sapiens
85 RPA3 6119
Proximity Label-MS Homo sapiens
86 CHRDL1 91851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 PLA2G2D  
Affinity Capture-MS Homo sapiens
88 MFN2 9927
Proximity Label-MS Homo sapiens
89 CCNG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 POGLUT1 56983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 WFDC10A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 ANGPT2 285
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 XRCC5 7520
Affinity Capture-MS Homo sapiens
94 FGFRL1 53834
Affinity Capture-MS Homo sapiens
95 RAPSN  
Affinity Capture-MS Homo sapiens
96 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 MYCBP 26292
Co-fractionation Homo sapiens
98 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
99 FAM81A  
Affinity Capture-MS Homo sapiens
100 RBFA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 SKIL  
Two-hybrid Homo sapiens
102 GAS2L1 10634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 HECTD3 79654
Co-fractionation Homo sapiens
104 ABHD10 55347
Affinity Capture-MS Homo sapiens
105 SEMA3C 10512
Affinity Capture-MS Homo sapiens
106 CHST5  
Affinity Capture-MS Homo sapiens
107 RPS4X 6191
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 WNT2  
Affinity Capture-MS Homo sapiens
110 OVOL2  
Affinity Capture-MS Homo sapiens
111 FST 10468
Affinity Capture-MS Homo sapiens
112 DEFB107A  
Affinity Capture-MS Homo sapiens
113 EGFL6 25975
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 PARD6B 84612
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 TEFM  
Affinity Capture-MS Homo sapiens
116 BRD1 23774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
118 PTRF 284119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 TIMP3 7078
Affinity Capture-MS Homo sapiens
120 MRPS9 64965
Affinity Capture-MS Homo sapiens
121 GREM2 64388
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ZZEF1 is involved
No pathways found





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