Gene description for Tmed10
Gene name transmembrane emp24-like trafficking protein 10 (yeast)
Gene symbol Tmed10
Other names/aliases 1110014C03Rik
Tmp21
p23
p24delta1
Species Mus musculus
 Database cross references - Tmed10
ExoCarta ExoCarta_68581
Vesiclepedia VP_68581
Entrez Gene 68581
UniProt Q9D1D4  
 Tmed10 identified in exosomes derived from the following tissue/cell type
Pancreatic cells 19351151    
 Gene ontology annotations for Tmed10
Molecular Function
    protein transmembrane transporter activity GO:0008320 ISO
    protein transmembrane transporter activity GO:0008320 ISS
    syntaxin binding GO:0019905 IEA
    syntaxin binding GO:0019905 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 ISO
    intracellular protein transport GO:0006886 TAS
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISS
    Golgi organization GO:0007030 IBA
    Golgi organization GO:0007030 IDA
    Golgi organization GO:0007030 ISO
    positive regulation of interleukin-1 production GO:0032732 IMP
    positive regulation of interleukin-1 production GO:0032732 ISO
    positive regulation of interleukin-1 production GO:0032732 ISS
    COPI-coated vesicle budding GO:0035964 ISO
    COPI-coated vesicle budding GO:0035964 ISS
    regulated exocytosis GO:0045055 IEP
    vesicle targeting, to, from or within Golgi GO:0048199 IEP
    positive regulation of protein secretion GO:0050714 IMP
    positive regulation of protein secretion GO:0050714 ISO
    positive regulation of protein secretion GO:0050714 ISS
    protein transmembrane transport GO:0071806 IEA
    protein localization to ERGIC GO:0106272 ISO
    protein localization to ERGIC GO:0106272 ISS
    cytosol to ERGIC protein transport GO:0106273 ISO
    cytosol to ERGIC protein transport GO:0106273 ISS
    regulation of amyloid-beta formation GO:1902003 IEA
    regulation of amyloid-beta formation GO:1902003 ISO
    negative regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process GO:1902960 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISO
    cis-Golgi network GO:0005801 ISO
    cis-Golgi network GO:0005801 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    COPII-coated ER to Golgi transport vesicle GO:0030134 IBA
    COPI-coated vesicle GO:0030137 IBA
    COPI-coated vesicle GO:0030137 ISO
    trans-Golgi network transport vesicle GO:0030140 ISS
    transport vesicle membrane GO:0030658 IEA
    secretory granule membrane GO:0030667 IDA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
    melanosome GO:0042470 IEA
    zymogen granule membrane GO:0042589 IDA
    zymogen granule membrane GO:0042589 ISO
    zymogen granule membrane GO:0042589 ISS
    gamma-secretase complex GO:0070765 IDA
    gamma-secretase complex GO:0070765 ISO
    gamma-secretase complex GO:0070765 ISS
 Experiment description of studies that identified Tmed10 in exosomes
1
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Tmed10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP3S1 1176
Affinity Capture-MS Homo sapiens
2 GSN 2934
Affinity Capture-MS Homo sapiens
3 DGCR8  
Affinity Capture-MS Homo sapiens
4 TLN2 83660
Affinity Capture-MS Homo sapiens
5 RPL37 6167
Affinity Capture-MS Homo sapiens
6 RBBP6 5930
Affinity Capture-MS Homo sapiens
7 KRT73 319101
Affinity Capture-MS Homo sapiens
8 WDR6 11180
Affinity Capture-MS Homo sapiens
9 SPTAN1 6709
Affinity Capture-MS Homo sapiens
10 IRGQ 126298
Affinity Capture-MS Homo sapiens
11 SARS 6301
Affinity Capture-MS Homo sapiens
12 TMED9 54732
Affinity Capture-MS Homo sapiens
13 RIF1  
Affinity Capture-MS Homo sapiens
14 PACSIN1  
Affinity Capture-MS Homo sapiens
15 MYCBP2 23077
Affinity Capture-MS Homo sapiens
16 TMED3 23423
Affinity Capture-MS Homo sapiens
17 DAP3 7818
Affinity Capture-MS Homo sapiens
18 MRPS35 60488
Affinity Capture-MS Homo sapiens
19 ARPC2 10109
Affinity Capture-MS Homo sapiens
20 STOML2 30968
Affinity Capture-MS Homo sapiens
21 TMED4 222068
Affinity Capture-MS Homo sapiens
22 RIMS1  
Affinity Capture-MS Homo sapiens
23 TMED5 50999
Affinity Capture-MS Homo sapiens
24 Tsc1  
Affinity Capture-MS Mus musculus
25 PNP 4860
Affinity Capture-MS Homo sapiens
26 BLZF1  
Affinity Capture-MS Homo sapiens
27 DBN1 1627
Affinity Capture-MS Homo sapiens
28 ZZEF1 23140
Affinity Capture-MS Homo sapiens
29 ATP6V1D 51382
Affinity Capture-MS Homo sapiens
30 ERLIN2 11160
Affinity Capture-MS Homo sapiens
31 PDIA3 2923
Affinity Capture-MS Homo sapiens
32 AKR7A2 8574
Affinity Capture-MS Homo sapiens
33 LUZP1 7798
Affinity Capture-MS Homo sapiens
34 RPUSD4 84881
Affinity Capture-MS Homo sapiens
35 ZNF281  
Affinity Capture-MS Homo sapiens
36 IPP  
Affinity Capture-MS Homo sapiens
37 TMOD1  
Affinity Capture-MS Homo sapiens
38 RBM7  
Affinity Capture-MS Homo sapiens
39 MYO1C 4641
Affinity Capture-MS Homo sapiens
40 GDAP1 54332
Affinity Capture-MS Homo sapiens
41 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
42 RB1CC1 9821
Affinity Capture-MS Homo sapiens
43 Tmem173  
Proximity Label-MS Mus musculus
44 SRRT 51593
Affinity Capture-MS Homo sapiens
45 TSPO 706
Affinity Capture-MS Homo sapiens
46 YTHDC1  
Affinity Capture-MS Homo sapiens
47 TPM1 7168
Affinity Capture-MS Homo sapiens
48 NDUFS8 4728
Affinity Capture-MS Homo sapiens
49 TARSL2 123283
Affinity Capture-MS Homo sapiens
50 PFKL 5211
Affinity Capture-MS Homo sapiens
51 EIF4B 1975
Affinity Capture-MS Homo sapiens
52 PCGF5  
Affinity Capture-MS Homo sapiens
53 LIMA1 51474
Affinity Capture-MS Homo sapiens
54 MDN1 23195
Affinity Capture-MS Homo sapiens
55 ARHGAP11A  
Affinity Capture-MS Homo sapiens
56 UHRF1BP1 54887
Affinity Capture-MS Homo sapiens
57 IFT88 8100
Affinity Capture-MS Homo sapiens
58 Nbr1  
Two-hybrid Mus musculus
59 LPIN3 64900
Affinity Capture-MS Homo sapiens
60 CFL2 1073
Affinity Capture-MS Homo sapiens
61 TMED7 51014
Affinity Capture-MS Homo sapiens
62 KIAA1522 57648
Affinity Capture-MS Homo sapiens
63 TMED2 10959
Affinity Capture-MS Homo sapiens
64 ARPC5L 81873
Affinity Capture-MS Homo sapiens
65 ANK3  
Affinity Capture-MS Homo sapiens
66 CCDC88A 55704
Affinity Capture-MS Homo sapiens
67 C6orf120  
Affinity Capture-MS Homo sapiens
68 MSH3 4437
Affinity Capture-MS Homo sapiens
69 UBE2G1 7326
Affinity Capture-MS Homo sapiens
70 Hyou1 12282
Co-fractionation Mus musculus
71 INTS5 80789
Affinity Capture-MS Homo sapiens
72 C19orf70  
Affinity Capture-MS Homo sapiens
73 ACTG1 71
Affinity Capture-MS Homo sapiens
74 SIRT1  
Affinity Capture-MS Homo sapiens
75 TMED1 11018
Affinity Capture-MS Homo sapiens
76 Fancd2  
Affinity Capture-MS Mus musculus
77 HMGB2 3148
Affinity Capture-MS Homo sapiens
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