Gene description for UBE2G1
Gene name ubiquitin-conjugating enzyme E2G 1
Gene symbol UBE2G1
Other names/aliases E217K
UBC7
UBE2G
Species Homo sapiens
 Database cross references - UBE2G1
ExoCarta ExoCarta_7326
Vesiclepedia VP_7326
Entrez Gene 7326
HGNC 12482
MIM 601569
UniProt P62253  
 UBE2G1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Urine 19056867    
 Gene ontology annotations for UBE2G1
Molecular Function
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    ATP binding GO:0005524 IEA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ligase activity GO:0016874 IEA
Biological Process
    protein K48-linked ubiquitination GO:0070936 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    protein K63-linked ubiquitination GO:0070534 IDA
    cellular protein catabolic process GO:0044257 IMP
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified UBE2G1 in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|CD9|FLOT1
EV Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for UBE2G1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2E1  
Reconstituted Complex Homo sapiens
2 UBE3A 7337
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 PARK2  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which UBE2G1 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome





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