Experiment description of studies that identified RNF11 in exosomes

1 Experiment ID 231 MISEV standards ✘ EV Biophysical techniques ✔ Alix|CD63|CD9 EV Enriched markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 232 MISEV standards ✘ EV Biophysical techniques ✘ EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 233 MISEV standards ✘ EV Biophysical techniques ✘ EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 13 MISEV standards ✔ IEM EV Biophysical techniques ✔ Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 15326289     Organism Homo sapiens Experiment description Identification and proteomic profiling of exosomes in human urine. Authors Pisitkun T, Shen RF, Knepper MA Journal name PNAS Publication year 2004 Sample Urine Sample name Urine - Normal Isolation/purification methods Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA XP] Western blotting

5 Experiment ID 63 MISEV standards ✘ EV Biophysical techniques ✔ AQP2 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for RNF11

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RPS27A 6233 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 2 TNIP1 10318 Affinity Capture-MS Homo sapiens 3 CBLB 868 Affinity Capture-MS Homo sapiens 4 UBQLN4 56893 Affinity Capture-MS Homo sapiens 5 ZFYVE9   Affinity Capture-MS Homo sapiens 6 QARS 5859 Affinity Capture-MS Homo sapiens 7 NDP52   Affinity Capture-MS Homo sapiens 8 WWP1 11059 Affinity Capture-MS Homo sapiens 9 PDCD6IP 10015 Affinity Capture-MS Homo sapiens 10 RABGEF1 27342 Affinity Capture-MS Homo sapiens 11 USP5 8078 Affinity Capture-MS Homo sapiens 12 STAM2 10254 Affinity Capture-MS Homo sapiens 13 IKBKG 8517 Affinity Capture-MS Homo sapiens 14 EPS15 2060 Two-hybrid Homo sapiens 15 UBC 7316 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 16 ZFYVE16   Affinity Capture-MS Homo sapiens 17 UBB 7314 Affinity Capture-MS Homo sapiens 18 UBE2D4   Affinity Capture-MS Homo sapiens 19 OPTN 10133 Affinity Capture-MS Homo sapiens 20 ZNF350   Two-hybrid Homo sapiens 21 CCDC127   Affinity Capture-MS Homo sapiens 22 WWP2 11060 Affinity Capture-MS Homo sapiens 23 DCUN1D1 54165 Affinity Capture-MS Homo sapiens 24 EPN3 55040 Affinity Capture-MS Homo sapiens 25 AMSH-LP   Two-hybrid Homo sapiens 26 DKFZP547N043   Affinity Capture-MS Homo sapiens 27 STAMBP 10617 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 28 NEDD4L 23327 Affinity Capture-MS Homo sapiens 29 POLI   Affinity Capture-MS Homo sapiens View the network

Pathways in which RNF11 is involved

No pathways found