Gene description for RNF11
Gene name ring finger protein 11
Gene symbol RNF11
Other names/aliases CGI-123
SID1669
Species Homo sapiens
 Database cross references - RNF11
ExoCarta ExoCarta_26994
Entrez Gene 26994
HGNC 10056
MIM 612598
UniProt Q9Y3C5  
 RNF11 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for RNF11
Molecular Function
    DNA binding GO:0003677 TAS
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IDA
    protein autoubiquitination GO:0051865 IDA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    recycling endosome GO:0055037 IEA
    ubiquitin ligase complex GO:0000151 IDA
    nucleus GO:0005634 IEA
    early endosome GO:0005769 IEA
 Experiment description of studies that identified RNF11 in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
5
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RNF11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPS27A 6233
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
2 TNIP1 10318
Affinity Capture-MS Homo sapiens
3 UBQLN4 56893
Affinity Capture-MS Homo sapiens
4 ZFYVE9  
Affinity Capture-MS Homo sapiens
5 QARS 5859
Affinity Capture-MS Homo sapiens
6 NDP52  
Affinity Capture-MS Homo sapiens
7 WWP1 11059
Affinity Capture-MS Homo sapiens
8 RP42  
Affinity Capture-MS Homo sapiens
9 PDCD6IP 10015
Affinity Capture-MS Homo sapiens
10 USP5 8078
Affinity Capture-MS Homo sapiens
11 STAM2 10254
Affinity Capture-MS Homo sapiens
12 IKBKG 8517
Affinity Capture-MS Homo sapiens
13 EPS15 2060
Two-hybrid Homo sapiens
14 UBC 7316
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
15 ZFYVE16  
Affinity Capture-MS Homo sapiens
16 WWP2 11060
Affinity Capture-MS Homo sapiens
17 UBE2D4  
Affinity Capture-MS Homo sapiens
18 CBLB  
Affinity Capture-MS Homo sapiens
19 OPTN 10133
Affinity Capture-MS Homo sapiens
20 ZNF350  
Two-hybrid Homo sapiens
21 CCDC127  
Affinity Capture-MS Homo sapiens
22 UBB 7314
Affinity Capture-MS Homo sapiens
23 RABGEF1  
Affinity Capture-MS Homo sapiens
24 EPN3 55040
Affinity Capture-MS Homo sapiens
25 AMSH-LP  
Two-hybrid Homo sapiens
26 DKFZP547N043  
Affinity Capture-MS Homo sapiens
27 STAMBP 10617
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 NEDD4L 23327
Affinity Capture-MS Homo sapiens
29 POLI  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RNF11 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here