Gene description for AP2S1
Gene name adaptor-related protein complex 2, sigma 1 subunit
Gene symbol AP2S1
Other names/aliases AP17
CLAPS2
FBH3
FBHOk
HHC3
Species Homo sapiens
 Database cross references - AP2S1
ExoCarta ExoCarta_1175
Vesiclepedia VP_1175
Entrez Gene 1175
HGNC 565
MIM 602242
UniProt P53680  
 AP2S1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for AP2S1
Molecular Function
    protein binding GO:0005515 IPI
    clathrin adaptor activity GO:0035615 TAS
Biological Process
    intracellular protein transport GO:0006886 IEA
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    regulation of endocytosis GO:0030100 TAS
    clathrin coat assembly GO:0048268 TAS
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    synaptic vesicle endocytosis GO:0048488 NAS
    clathrin-dependent endocytosis GO:0072583 NAS
    clathrin-dependent endocytosis GO:0072583 TAS
    postsynaptic neurotransmitter receptor internalization GO:0098884 NAS
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    cytoplasmic side of plasma membrane GO:0009898 NAS
    AP-2 adaptor complex GO:0030122 NAS
    AP-2 adaptor complex GO:0030122 TAS
    endocytic vesicle membrane GO:0030666 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    endolysosome membrane GO:0036020 TAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    clathrin-coated endocytic vesicle GO:0045334 NAS
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
 Experiment description of studies that identified AP2S1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AP2S1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NAA25 80018
Affinity Capture-MS Homo sapiens
2 VCAM1 7412
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 PAICS 10606
Affinity Capture-MS Homo sapiens
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 MECP2 4204
Affinity Capture-MS Homo sapiens
7 FOXA1  
Affinity Capture-MS Homo sapiens
8 C9orf72  
Affinity Capture-MS Homo sapiens
9 AP2B1 163
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 Smo  
Affinity Capture-MS Mus musculus
11 PLCB3 5331
Affinity Capture-MS Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 ITSN1 6453
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GRB2 2885
Affinity Capture-MS Homo sapiens
15 DVL2 1856
Affinity Capture-MS Homo sapiens
16 AAK1 22848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RNF11 26994
Affinity Capture-MS Homo sapiens
18 UQCC1 55245
Affinity Capture-MS Homo sapiens
19 ITSN2 50618
Affinity Capture-MS Homo sapiens
20 C15orf59  
Affinity Capture-MS Homo sapiens
21 OCRL 4952
Affinity Capture-MS Homo sapiens
22 RPA2 6118
Proximity Label-MS Homo sapiens
23 ARHGEF1 9138
Affinity Capture-MS Homo sapiens
24 RAB25 57111
Affinity Capture-MS Homo sapiens
25 AP2A2 161
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
27 TDGF1  
Two-hybrid Homo sapiens
28 MIB1 57534
Proximity Label-MS Homo sapiens
29 EPS15 2060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CUL3 8452
Affinity Capture-MS Homo sapiens
31 KHK 3795
Affinity Capture-MS Homo sapiens
32 IKBIP 121457
Affinity Capture-MS Homo sapiens
33 Numb  
Affinity Capture-MS Mus musculus
34 UBC 7316
Reconstituted Complex Homo sapiens
35 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
37 MANF 7873
Affinity Capture-MS Homo sapiens
38 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 DIEXF  
Affinity Capture-MS Homo sapiens
40 CLTB 1212
Affinity Capture-MS Homo sapiens
41 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
42 ACACA 31
Negative Genetic Homo sapiens
43 SNX9 51429
Affinity Capture-MS Homo sapiens
44 CUL9  
Affinity Capture-MS Homo sapiens
45 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 AMPH 273
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 FCHO2 115548
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 C10orf88  
Affinity Capture-MS Homo sapiens
49 AAGAB  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MAPK6  
Affinity Capture-MS Homo sapiens
51 NECAP2 55707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 KRAS 3845
Negative Genetic Homo sapiens
53 MYO6 4646
Proximity Label-MS Homo sapiens
54 Dctn3  
Affinity Capture-MS Mus musculus
55 RAN 5901
Affinity Capture-MS Homo sapiens
56 RALBP1 10928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 ZCCHC10  
Affinity Capture-MS Homo sapiens
58 CLTA 1211
Affinity Capture-MS Homo sapiens
59 AP2A1 160
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 REPS1 85021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 AP1B1 162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 EPHA2 1969
Affinity Capture-Western Homo sapiens
63 BMP2K 55589
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 AP1G1 164
Affinity Capture-Western Homo sapiens
65 RUNDC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ITGA4 3676
Affinity Capture-MS Homo sapiens
67 SGIP1  
Affinity Capture-MS Homo sapiens
68 STON2 85439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 PAK7  
Affinity Capture-MS Homo sapiens
70 C15orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 NECAP1 25977
Affinity Capture-MS Homo sapiens
72 EPS15L1 58513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 GPBP1L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 HUWE1 10075
Affinity Capture-MS Homo sapiens
75 ARFGAP1 55738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 POLQ  
Affinity Capture-MS Homo sapiens
77 RPA3 6119
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AP2S1 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Axon guidance TAS Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling TAS Reactome
Cargo recognition for clathrin-mediated endocytosis TAS Reactome
Clathrin-mediated endocytosis TAS Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
EPH-ephrin mediated repulsion of cells TAS Reactome
EPH-Ephrin signaling TAS Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity TAS Reactome
HIV Infection TAS Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
L1CAM interactions TAS Reactome
L1CAM interactions IEA Reactome
LDL clearance TAS Reactome
Membrane Trafficking TAS Reactome
MHC class II antigen presentation TAS Reactome
Nef Mediated CD4 Down-regulation TAS Reactome
Nef Mediated CD8 Down-regulation TAS Reactome
Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters TAS Reactome
Nervous system development TAS Reactome
Nervous system development IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
PCP/CE pathway TAS Reactome
Plasma lipoprotein assembly, remodeling, and clearance TAS Reactome
Plasma lipoprotein clearance TAS Reactome
Potential therapeutics for SARS TAS Reactome
Recycling pathway of L1 TAS Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
Retrograde neurotrophin signalling TAS Reactome
SARS-CoV Infections TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRK1 (TRKA) TAS Reactome
Signaling by NTRKs IEA Reactome
Signaling by NTRKs TAS Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by WNT TAS Reactome
The role of Nef in HIV-1 replication and disease pathogenesis TAS Reactome
Trafficking of AMPA receptors TAS Reactome
Trafficking of GluR2-containing AMPA receptors TAS Reactome
Transmission across Chemical Synapses TAS Reactome
Transport of small molecules TAS Reactome
Vesicle-mediated transport TAS Reactome
Viral Infection Pathways TAS Reactome
VLDLR internalisation and degradation TAS Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 TAS Reactome
WNT5A-dependent internalization of FZD4 TAS Reactome





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