Gene description for AMPH
Gene name amphiphysin
Gene symbol AMPH
Other names/aliases AMPH1
Species Homo sapiens
 Database cross references - AMPH
ExoCarta ExoCarta_273
Vesiclepedia VP_273
Entrez Gene 273
HGNC 471
MIM 600418
UniProt P49418  
 AMPH identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for AMPH
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
    phospholipid binding GO:0005543 IDA
Biological Process
    endocytosis GO:0006897 TAS
    chemical synaptic transmission GO:0007268 TAS
    synaptic vesicle endocytosis GO:0048488 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    synaptic vesicle GO:0008021 IBA
    actin cytoskeleton GO:0015629 TAS
    synaptic vesicle membrane GO:0030672 IEA
    leading edge membrane GO:0031256 IDA
 Experiment description of studies that identified AMPH in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AMPH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP2S1 1175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GPM6A 2823
Affinity Capture-MS Homo sapiens
3 Bin1  
Reconstituted Complex Mus musculus
4 MANEA 79694
Affinity Capture-Western Homo sapiens
5 TNK2 10188
Affinity Capture-Western Homo sapiens
6 DNM1 1759
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 AP2B1 163
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SYNM 23336
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 ITSN1 6453
Affinity Capture-Western Homo sapiens
10 CCDC67  
Two-hybrid Homo sapiens
11 VTN 7448
Reconstituted Complex Homo sapiens
12 Dnm1 13429
Reconstituted Complex Mus musculus
13 Ap2a1  
Affinity Capture-Western Rattus norvegicus
14 AP2A1 160
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 BIN2 51411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 AP1G1 164
Affinity Capture-Western Homo sapiens
17 CABIN1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 UBC 7316
Reconstituted Complex Homo sapiens
19 CALM1 801
Affinity Capture-Western Homo sapiens
20 CDK5 1020
Biochemical Activity Homo sapiens
21 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TP73  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 PLD1 5337
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ARL16  
Two-hybrid Homo sapiens
26 ITSN2 50618
Two-hybrid Homo sapiens
27 AAGAB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MAPK6  
Two-hybrid Homo sapiens
29 TERF1 7013
Two-hybrid Homo sapiens
30 MAPT  
Reconstituted Complex Homo sapiens
31 PTK2 5747
Affinity Capture-Western Homo sapiens
32 GLUL 2752
Affinity Capture-MS Homo sapiens
33 REPS1 85021
Affinity Capture-Western Homo sapiens
34 DYRK1A 1859
Biochemical Activity Homo sapiens
35 SYNJ1 8867
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
36 AP2A2 161
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 Ppp3cb  
Biochemical Activity Rattus norvegicus
39 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 AP1B1 162
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CDK5R1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
42 SH3GL2  
Reconstituted Complex Homo sapiens
43 REPS2  
Affinity Capture-Western Homo sapiens
44 LGMN 5641
Biochemical Activity Homo sapiens
45 SGIP1  
Affinity Capture-Western Homo sapiens
46 CLIP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 KHDRBS1 10657
Protein-peptide Homo sapiens
48 CLTC 1213
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
49 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 DNM2 1785
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
51 Cltc 67300
Reconstituted Complex Mus musculus
52 PLD2 5338
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
53 BIN1 274
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PPP3CC 5533
Two-hybrid Homo sapiens
55 SYN1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which AMPH is involved
PathwayEvidenceSource
Clathrin-mediated endocytosis TAS Reactome
Membrane Trafficking TAS Reactome
Vesicle-mediated transport TAS Reactome





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