Gene description for MANEA
Gene name mannosidase, endo-alpha
Gene symbol MANEA
Other names/aliases ENDO
hEndo
Species Homo sapiens
 Database cross references - MANEA
ExoCarta ExoCarta_79694
Vesiclepedia VP_79694
Entrez Gene 79694
HGNC 21072
MIM 612327
UniProt Q5SRI9  
 MANEA identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MANEA
Molecular Function
    alpha-mannosidase activity GO:0004559 IBA
    glycoprotein endo-alpha-1,2-mannosidase activity GO:0004569 IDA
    glycoprotein endo-alpha-1,2-mannosidase activity GO:0004569 TAS
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IDA
 Experiment description of studies that identified MANEA in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MANEA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRP3  
Affinity Capture-MS Homo sapiens
2 HSPA13 6782
Affinity Capture-MS Homo sapiens
3 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LAMB1 3912
Affinity Capture-MS Homo sapiens
5 NID2 22795
Affinity Capture-MS Homo sapiens
6 BSCL2  
Affinity Capture-MS Homo sapiens
7 MAN2B2 23324
Affinity Capture-MS Homo sapiens
8 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TRHDE 29953
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PSG1  
Affinity Capture-MS Homo sapiens
11 CHRNA4  
Affinity Capture-MS Homo sapiens
12 TTYH1  
Affinity Capture-MS Homo sapiens
13 PRD  
Affinity Capture-Western Homo sapiens
14 HSD11B1 3290
Affinity Capture-MS Homo sapiens
15 OLFM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SLC39A4 55630
Affinity Capture-MS Homo sapiens
17 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LAMA1 284217
Affinity Capture-MS Homo sapiens
19 MME 4311
Affinity Capture-MS Homo sapiens
20 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ATRN 8455
Affinity Capture-MS Homo sapiens
22 ASGR2  
Affinity Capture-MS Homo sapiens
23 TMEM30B 161291
Affinity Capture-MS Homo sapiens
24 AOC3 8639
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 AGRN 375790
Affinity Capture-MS Homo sapiens
26 ANTXR1 84168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 GGT7 2686
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 RET 5979
Affinity Capture-MS Homo sapiens
29 CLU 1191
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 EMR4P  
Affinity Capture-MS Homo sapiens
31 UGT1A10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SLC22A9  
Affinity Capture-MS Homo sapiens
33 PSMD14 10213
Affinity Capture-MS Homo sapiens
34 AMPH 273
Affinity Capture-Western Homo sapiens
35 LAMC1 3915
Affinity Capture-MS Homo sapiens
36 CPVL 54504
Affinity Capture-MS Homo sapiens
37 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
38 PLD3 23646
Affinity Capture-MS Homo sapiens
39 GAL3ST2  
Affinity Capture-MS Homo sapiens
40 PLXNC1 10154
Affinity Capture-MS Homo sapiens
41 SCARA3  
Affinity Capture-MS Homo sapiens
42 ARSF 416
Affinity Capture-MS Homo sapiens
43 LAMB2 3913
Affinity Capture-MS Homo sapiens
44 MAN2B1 4125
Affinity Capture-MS Homo sapiens
45 LAMA5 3911
Affinity Capture-MS Homo sapiens
46 GRIN1 2902
Affinity Capture-MS Homo sapiens
47 TRDN 10345
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ITGB7 3695
Affinity Capture-MS Homo sapiens
49 CRELD1 78987
Affinity Capture-MS Homo sapiens
50 PCYOX1L 78991
Affinity Capture-MS Homo sapiens
51 DNM2 1785
Reconstituted Complex Homo sapiens
52 SIDT2 51092
Affinity Capture-MS Homo sapiens
53 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 LAMA4 3910
Affinity Capture-MS Homo sapiens
55 CNDP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 TAS1R2  
Affinity Capture-MS Homo sapiens
57 SIAE 54414
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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