Gene description for CDK5
Gene name cyclin-dependent kinase 5
Gene symbol CDK5
Other names/aliases LIS7
PSSALRE
Species Homo sapiens
 Database cross references - CDK5
ExoCarta ExoCarta_1020
Vesiclepedia VP_1020
Entrez Gene 1020
HGNC 1774
MIM 123831
UniProt Q00535  
 CDK5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for CDK5
Molecular Function
    tau-protein kinase activity GO:0050321 ISS
    ErbB-2 class receptor binding GO:0005176 ISS
    p53 binding GO:0002039 IEA
    protein binding GO:0005515 IPI
    ErbB-3 class receptor binding GO:0043125 ISS
    ephrin receptor binding GO:0046875 IEA
    cytoskeletal protein binding GO:0008092 IEA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IEA
    ATP binding GO:0005524 IEA
    kinase activity GO:0016301 ISS
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 TAS
    acetylcholine receptor activator activity GO:0030549 ISS
Biological Process
    neuron differentiation GO:0030182 TAS
    axon extension GO:0048675 TAS
    cell cycle GO:0007049 IEA
    peptidyl-threonine phosphorylation GO:0018107 IEA
    negative regulation of axon extension GO:0030517 IEA
    neuron projection development GO:0031175 ISS
    synaptic vesicle endocytosis GO:0048488 TAS
    skeletal muscle tissue development GO:0007519 IEA
    phosphorylation GO:0016310 IDA
    negative regulation of cell cycle GO:0045786 IEA
    regulation of cell migration GO:0030334 IEA
    positive regulation of protein targeting to membrane GO:0090314 IEA
    positive regulation of neuron apoptotic process GO:0043525 ISS
    negative regulation of protein ubiquitination GO:0031397 IEA
    protein localization to synapse GO:0035418 IEA
    layer formation in cerebral cortex GO:0021819 IEA
    behavioral response to cocaine GO:0048148 IEA
    synaptic transmission GO:0007268 TAS
    cell division GO:0051301 IEA
    regulated secretory pathway GO:0045055 IEA
    synaptic transmission, dopaminergic GO:0001963 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    regulation of apoptotic process GO:0042981 TAS
    cortical actin cytoskeleton organization GO:0030866 IEA
    receptor clustering GO:0043113 IEA
    negative regulation of proteolysis GO:0045861 IMP
    peptidyl-serine phosphorylation GO:0018105 IDA
    Schwann cell development GO:0014044 IEA
    regulation of synaptic vesicle recycling GO:1903421 NAS
    positive regulation of protein kinase activity GO:0045860 IEA
    regulation of excitatory postsynaptic membrane potential GO:0060079 IEA
    regulation of cell cycle arrest GO:0071156 TAS
    positive regulation of receptor activity GO:2000273 IEA
    cerebellar cortex formation GO:0021697 IEA
    synaptic vesicle exocytosis GO:0016079 TAS
    protein autophosphorylation GO:0046777 IEA
    embryo development GO:0009790 IEA
    synapse assembly GO:0007416 TAS
    regulation of dendritic spine morphogenesis GO:0061001 ISS
    neuron migration GO:0001764 TAS
    sensory perception of pain GO:0019233 IEA
    cell proliferation GO:0008283 TAS
    serine phosphorylation of STAT3 protein GO:0033136 IEA
    cell-matrix adhesion GO:0007160 IEA
    rhythmic process GO:0048511 IEA
    negative regulation of synaptic plasticity GO:0031914 IEA
    positive regulation of calcium ion-dependent exocytosis GO:0045956 IEA
    positive regulation of protein binding GO:0032092 IEA
    negative regulation of protein export from nucleus GO:0046826 IEA
    hippocampus development GO:0021766 IEA
    dendrite morphogenesis GO:0048813 IEA
    visual learning GO:0008542 IEA
    receptor catabolic process GO:0032801 IEA
    motor neuron axon guidance GO:0008045 IEA
    intracellular protein transport GO:0006886 IEA
    axon guidance GO:0007411 TAS
    regulation of synaptic plasticity GO:0048167 TAS
    positive regulation of actin cytoskeleton reorganization GO:2000251 TAS
    central nervous system neuron development GO:0021954 IEA
    oligodendrocyte differentiation GO:0048709 IDA
    neuron apoptotic process GO:0051402 TAS
    blood coagulation GO:0007596 TAS
    calcium ion import GO:0070509 IEA
    nucleocytoplasmic transport GO:0006913 IEA
    corpus callosum development GO:0022038 IEA
    synaptic transmission, glutamatergic GO:0035249 IEA
    negative regulation of neuron death GO:1901215 IDA
Subcellular Localization
    cytoplasm GO:0005737 ISS
    axon GO:0030424 ISS
    dendrite GO:0030425 ISS
    nucleus GO:0005634 ISS
    filopodium GO:0030175 IEA
    membrane GO:0016020 ISS
    postsynaptic membrane GO:0045211 IEA
    neuronal cell body GO:0043025 ISS
    growth cone GO:0030426 ISS
    cytoskeleton GO:0005856 IEA
    neuromuscular junction GO:0031594 ISS
    postsynaptic density GO:0014069 ISS
    perikaryon GO:0043204 IEA
    cell junction GO:0030054 IEA
    lamellipodium GO:0030027 IEA
    cytosol GO:0005829 TAS
    cyclin-dependent protein kinase 5 holoenzyme complex GO:0016533 IEA
 Experiment description of studies that identified CDK5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDK5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCND2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 STXBP1 6812
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
3 PCNA 5111
Invitro Homo sapiens
4 CTNNB1 1499
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 CDK5R1  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 CCNG1  
Affinity Capture-MS Homo sapiens
7 GAK 2580
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 LMTK2 22853
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 CDK5R2 8941
Invivo Homo sapiens
10 CDK5RAP1  
Invivo Homo sapiens
Invitro Homo sapiens
11 SET 6418
Affinity Capture-MS Homo sapiens
12 STX1A 6804
Invitro Homo sapiens
13 NES 10763
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
14 CDK5 1020
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
15 CABLES2  
Affinity Capture-Western Homo sapiens
16 NDEL1 81565
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
View the network image/svg+xml



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