Gene description for CDC25B
Gene name cell division cycle 25B
Gene symbol CDC25B
Other names/aliases -
Species Homo sapiens
 Database cross references - CDC25B
ExoCarta ExoCarta_994
Vesiclepedia VP_994
Entrez Gene 994
HGNC 1726
MIM 116949
UniProt P30305  
 CDC25B identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for CDC25B
Molecular Function
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity GO:0004725 TAS
Biological Process
    positive regulation of protein kinase activity GO:0045860 IEA
    mitotic cell cycle GO:0000278 TAS
    peptidyl-tyrosine dephosphorylation GO:0035335 TAS
    mitotic nuclear division GO:0007067 IEA
    positive regulation of mitotic cell cycle GO:0045931 IMP
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    protein phosphorylation GO:0006468 IDA
    positive regulation of cell proliferation GO:0008284 TAS
    oocyte maturation GO:0001556 IEA
    positive regulation of cytokinesis GO:0032467 IMP
    cell division GO:0051301 IEA
    female meiosis I GO:0007144 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    centrosome GO:0005813 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    spindle pole GO:0000922 IDA
 Experiment description of studies that identified CDC25B in exosomes
1
Experiment ID 236
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 193
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|CD9
EV Enriched markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDC25B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Invivo Homo sapiens
Invitro Homo sapiens
2 KRT35 3886
Affinity Capture-MS Homo sapiens
3 YWHAE 7531
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
4 YWHAQ 10971
Invitro Homo sapiens
Invivo Homo sapiens
5 AR 367
Invitro Homo sapiens
6 PHB2 11331
Affinity Capture-MS Homo sapiens
7 YWHAZ 7534
Invivo Homo sapiens
Invitro Homo sapiens
8 KRTHB5  
Affinity Capture-MS Homo sapiens
9 PCAF  
Reconstituted Complex Homo sapiens
10 CSNK2A1 1457
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 KRT31 3881
Affinity Capture-MS Homo sapiens
12 PGR  
Reconstituted Complex Homo sapiens
13 ESR1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 MELK  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 YWHAB 7529
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
16 CHEK1  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
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