Gene description for CDC25B
Gene name
cell division cycle 25B
Gene symbol
CDC25B
Other names/aliases
-
Species
Homo sapiens
Database cross references - CDC25B
ExoCarta
ExoCarta_994
Entrez Gene
994
HGNC
1726
MIM
116949
UniProt
P30305
CDC25B identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells
26054723
Urine
21595033
Urine
21595033
Urine
21595033
Gene ontology annotations for CDC25B
Experiment description of studies that identified CDC25B in exosomes
1
Experiment ID
236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
193
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
194
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of basement membrane nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
195
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of early IgA nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for CDC25B
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
YWHAH
7533
Invivo
Homo sapiens
Invitro
Homo sapiens
2
YWHAE
7531
Invivo
Homo sapiens
Invivo
Homo sapiens
Invitro
Homo sapiens
Invitro
Homo sapiens
3
YWHAQ
10971
Invitro
Homo sapiens
Invivo
Homo sapiens
4
KRTHA5
Affinity Capture-MS
Homo sapiens
5
AR
367
Invitro
Homo sapiens
6
PHB2
11331
Affinity Capture-MS
Homo sapiens
7
YWHAZ
7534
Invivo
Homo sapiens
Invitro
Homo sapiens
8
KRTHB5
Affinity Capture-MS
Homo sapiens
9
PCAF
Reconstituted Complex
Homo sapiens
10
CSNK2A1
1457
Biochemical Activity
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
11
KRT31
3881
Affinity Capture-MS
Homo sapiens
12
PGR
Reconstituted Complex
Homo sapiens
13
ESR1
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
14
MELK
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
15
YWHAB
7529
Invivo
Homo sapiens
Invivo
Homo sapiens
Invitro
Homo sapiens
Invitro
Homo sapiens
16
CHEK1
Biochemical Activity
Homo sapiens
Reconstituted Complex
Homo sapiens
View the network
image/svg+xml
Pathways in which CDC25B is involved