Gene description for PHB2
Gene name prohibitin 2
Gene symbol PHB2
Other names/aliases BAP
BCAP37
Bap37
PNAS-141
REA
p22
Species Homo sapiens
 Database cross references - PHB2
ExoCarta ExoCarta_11331
Entrez Gene 11331
HGNC 30306
MIM 610704
UniProt Q99623  
 PHB2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PHB2
Molecular Function
    protein C-terminus binding GO:0008022 IPI
    estrogen receptor binding GO:0030331 NAS
    protein N-terminus binding GO:0047485 IPI
    protein binding GO:0005515 IPI
    amide binding GO:0033218 IPI
    chromatin binding GO:0003682 IMP
Biological Process
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    sister chromatid cohesion GO:0007062 IDA
    mammary gland branching involved in thelarche GO:0060744 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of intracellular estrogen receptor signaling pathway GO:0033147 IEA
    protein stabilization GO:0050821 IMP
    transcription, DNA-templated GO:0006351 IEA
    negative regulation of mammary gland epithelial cell proliferation GO:0033600 IEA
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    regulation of branching involved in mammary gland duct morphogenesis GO:0060762 IEA
    mitochondrion organization GO:0007005 IMP
    regulation of complement activation GO:0030449 IDA
    mammary gland alveolus development GO:0060749 IEA
    positive regulation of cell cycle G1/S phase transition GO:1902808 IMP
    protein import into nucleus, translocation GO:0000060 IDA
    positive regulation of exit from mitosis GO:0031536 IMP
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 IMP
Subcellular Localization
    nucleus GO:0005634 ISS
    extracellular exosome GO:0070062 IDA
    mitochondrial outer membrane GO:0005741 IDA
    cell periphery GO:0071944 IDA
    nuclear matrix GO:0016363 IDA
    cytoplasm GO:0005737 IDA
    mitochondrial inner membrane GO:0005743 ISS
    chromatin GO:0000785 IDA
    protein complex GO:0043234 IDA
    cell surface GO:0009986 IDA
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified PHB2 in exosomes
1
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 258
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NELFCD 51497
Affinity Capture-MS Homo sapiens
2 SIRT2 22933
Affinity Capture-MS Homo sapiens
3 ESR1  
Two-hybrid Homo sapiens
4 TFE3  
Affinity Capture-MS Homo sapiens
5 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
6 RUNX3  
Affinity Capture-MS Homo sapiens
7 RIPK2  
Affinity Capture-MS Homo sapiens
8 NUP93 9688
Affinity Capture-MS Homo sapiens
9 CDC25B 994
Affinity Capture-MS Homo sapiens
10 NUP205 23165
Affinity Capture-MS Homo sapiens
11 TIMM50 92609
Affinity Capture-MS Homo sapiens
12 MMS19 64210
Affinity Capture-MS Homo sapiens
13 JUP 3728
Affinity Capture-MS Homo sapiens
14 DDX20  
Affinity Capture-MS Homo sapiens
15 EPPK1 83481
Affinity Capture-MS Homo sapiens
16 PTMA 5757
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 SERINC3 10955
Affinity Capture-MS Homo sapiens
18 USP15 9958
Affinity Capture-MS Homo sapiens
19 PDXDC1 23042
Affinity Capture-MS Homo sapiens
20 COPG1 22820
Affinity Capture-MS Homo sapiens
21 CTNNB1 1499
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PHB2 is involved
No pathways found





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