Gene description for Atp2a2
Gene name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Gene symbol Atp2a2
Other names/aliases 9530097L16Rik
D5Wsu150e
SERCA2
SERCA2B
mKIAA4195
Species Mus musculus
 Database cross references - Atp2a2
ExoCarta ExoCarta_11938
Vesiclepedia VP_11938
Entrez Gene 11938
UniProt O55143  
 Atp2a2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp2a2
Molecular Function
    calcium ion binding GO:0005509 ISO
    nucleotide binding GO:0000166 IEA
    protein C-terminus binding GO:0008022 ISO
    S100 protein binding GO:0044548 ISO
    calcium-transporting ATPase activity GO:0005388 ISO
    calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential GO:0086039 ISO
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    hydrolase activity GO:0016787 IEA
    ATP binding GO:0005524 ISO
    enzyme binding GO:0019899 ISO
    lutropin-choriogonadotropic hormone receptor binding GO:0031775 ISO
Biological Process
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 ISO
    response to peptide hormone GO:0043434 ISO
    organelle organization GO:0006996 IMP
    calcium ion transport from cytosol to endoplasmic reticulum GO:1903515 ISO
    positive regulation of endoplasmic reticulum calcium ion concentration GO:0032470 ISO
    endoplasmic reticulum calcium ion homeostasis GO:0032469 ISO
    ion transport GO:0006811 IEA
    relaxation of cardiac muscle GO:0055119 ISO
    regulation of muscle contraction GO:0006937 TAS
    regulation of cardiac muscle cell action potential involved in regulation of contraction GO:0098909 ISO
    calcium ion transport GO:0006816 ISO
    regulation of cardiac muscle cell membrane potential GO:0086036 ISO
    ER-nucleus signaling pathway GO:0006984 IMP
    transport GO:0006810 IEA
    calcium ion import into sarcoplasmic reticulum GO:1990036 ISO
    regulation of the force of heart contraction GO:0002026 IGI
    negative regulation of heart contraction GO:0045822 IGI
    calcium ion transmembrane transport GO:0070588 ISO
    T-tubule organization GO:0033292 IMP
    cellular calcium ion homeostasis GO:0006874 ISO
    regulation of calcium ion-dependent exocytosis of neurotransmitter GO:1903233 IMP
    sarcoplasmic reticulum calcium ion transport GO:0070296 ISO
    transition between fast and slow fiber GO:0014883 IDA
Subcellular Localization
    sarcoplasmic reticulum GO:0016529 TAS
    endoplasmic reticulum GO:0005783 ISO
    intracellular GO:0005622 IMP
    longitudinal sarcoplasmic reticulum GO:0014801 ISO
    sarcoplasmic reticulum membrane GO:0033017 IEA
    membrane GO:0016020 ISO
    intercalated disc GO:0014704 ISO
    integral component of membrane GO:0016021 IEA
    cell GO:0005623 IGI
    perinuclear region of cytoplasm GO:0048471 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    protein complex GO:0043234 ISO
    calcium ion-transporting ATPase complex GO:0090534 ISO
    vesicle membrane GO:0012506 ISO
    ribbon synapse GO:0097470 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 ISO
 Experiment description of studies that identified Atp2a2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp2a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atp2a2 is involved
PathwayEvidenceSource
Ion transport by P-type ATPases IEA Reactome
Reduction of cytosolic Ca++ levels IEA Reactome





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