Gene description for DHCR24
Gene name 24-dehydrocholesterol reductase
Gene symbol DHCR24
Other names/aliases DCE
Nbla03646
SELADIN1
seladin-1
Species Homo sapiens
 Database cross references - DHCR24
ExoCarta ExoCarta_1718
Vesiclepedia VP_1718
Entrez Gene 1718
HGNC 2859
MIM 606418
UniProt Q15392  
 DHCR24 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for DHCR24
Molecular Function
    delta24(24-1) sterol reductase activity GO:0000246 IBA
    delta24(24-1) sterol reductase activity GO:0000246 IMP
    protein binding GO:0005515 IPI
    oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor GO:0016628 IDA
    enzyme binding GO:0019899 IPI
    peptide antigen binding GO:0042605 IPI
    delta24-sterol reductase activity GO:0050614 EXP
    delta24-sterol reductase activity GO:0050614 TAS
    FAD binding GO:0071949 IEA
Biological Process
    cholesterol biosynthetic process GO:0006695 IMP
    cholesterol biosynthetic process GO:0006695 ISS
    cholesterol biosynthetic process GO:0006695 TAS
    Ras protein signal transduction GO:0007265 IEA
    protein localization GO:0008104 IEA
    steroid metabolic process GO:0008202 IBA
    negative regulation of cell population proliferation GO:0008285 IEA
    response to hormone GO:0009725 IEA
    tissue development GO:0009888 IMP
    male genitalia development GO:0030539 IEA
    plasminogen activation GO:0031639 IEA
    cholesterol biosynthetic process via desmosterol GO:0033489 IMP
    cholesterol biosynthetic process via desmosterol GO:0033489 TAS
    cholesterol biosynthetic process via lathosterol GO:0033490 TAS
    amyloid precursor protein catabolic process GO:0042987 IEA
    skin development GO:0043588 ISS
    membrane organization GO:0061024 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified DHCR24 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DHCR24
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSD17B11 51170
Proximity Label-MS Homo sapiens
2 UBL4A 8266
Affinity Capture-MS Homo sapiens
3 SYNGR1 9145
Affinity Capture-MS Homo sapiens
4 CCR4 1233
Two-hybrid Homo sapiens
5 ATP12A 479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 Atp2a2 11938
Affinity Capture-MS Mus musculus
7 HRAS 3265
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 C9orf78 51759
Affinity Capture-MS Homo sapiens
9 NDUFA4 4697
Affinity Capture-MS Homo sapiens
10 PTPN1 5770
Affinity Capture-MS Homo sapiens
11 COPA 1314
Affinity Capture-MS Homo sapiens
12 FFAR1  
Affinity Capture-MS Homo sapiens
13 SLC12A2 6558
Affinity Capture-MS Homo sapiens
14 RB1CC1 9821
Affinity Capture-MS Homo sapiens
15 GP5 2814
Affinity Capture-MS Homo sapiens
16 COQ9  
Affinity Capture-MS Homo sapiens
17 P2RY1  
Affinity Capture-MS Homo sapiens
18 AMFR 267
Affinity Capture-MS Homo sapiens
19 ERAP1 51752
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 POU5F1  
Affinity Capture-MS Homo sapiens
21 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
22 PIGH  
Affinity Capture-MS Homo sapiens
23 RTN1 6252
Affinity Capture-MS Homo sapiens
24 ATP2A3 489
Affinity Capture-MS Homo sapiens
25 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ATP13A3 79572
Affinity Capture-MS Homo sapiens
27 FUNDC1  
Affinity Capture-MS Homo sapiens
28 KIF14 9928
Affinity Capture-MS Homo sapiens
29 ATP1A3 478
Affinity Capture-MS Homo sapiens
30 NUP155 9631
Proximity Label-MS Homo sapiens
31 ATG9A 79065
Proximity Label-MS Homo sapiens
32 ACTA2 59
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 KCNK1  
Affinity Capture-MS Homo sapiens
34 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
35 LUZP1 7798
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 IFITM3 10410
Affinity Capture-MS Homo sapiens
37 COPE 11316
Affinity Capture-MS Homo sapiens
38 FUNDC2 65991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PSMD14 10213
Affinity Capture-MS Homo sapiens
40 FYN 2534
Affinity Capture-MS Homo sapiens
41 BTF3 689
Affinity Capture-MS Homo sapiens
42 CKAP5 9793
Affinity Capture-MS Homo sapiens
43 GPR182  
Affinity Capture-MS Homo sapiens
44 TMEM63A 9725
Affinity Capture-MS Homo sapiens
45 SEC61B 10952
Proximity Label-MS Homo sapiens
46 FUK 197258
Affinity Capture-MS Homo sapiens
47 REEP5 7905
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CISD3 284106
Affinity Capture-MS Homo sapiens
49 TMPO 7112
Affinity Capture-MS Homo sapiens
50 VIPR2  
Affinity Capture-MS Homo sapiens
51 SLC2A1 6513
Affinity Capture-MS Homo sapiens
52 TTYH1  
Affinity Capture-MS Homo sapiens
53 ENDOD1 23052
Affinity Capture-MS Homo sapiens
54 CANX 821
Affinity Capture-MS Homo sapiens
55 PANX1 24145
Proximity Label-MS Homo sapiens
56 CAV2 858
Affinity Capture-MS Homo sapiens
57 APOB 338
Affinity Capture-MS Homo sapiens
58 CD274 29126
Affinity Capture-MS Homo sapiens
59 CS 1431
Cross-Linking-MS (XL-MS) Homo sapiens
60 P2RY2 5029
Affinity Capture-MS Homo sapiens
61 HSD3B7 80270
Proximity Label-MS Homo sapiens
62 CUL3 8452
Affinity Capture-MS Homo sapiens
63 STIM1 6786
Proximity Label-MS Homo sapiens
64 EGFR 1956
Affinity Capture-MS Homo sapiens
65 LPAR1 1902
Affinity Capture-MS Homo sapiens
66 SYP  
Affinity Capture-MS Homo sapiens
67 STAT1 6772
Affinity Capture-MS Homo sapiens
68 CNR2  
Affinity Capture-MS Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 ATP2A1 487
Proximity Label-MS Homo sapiens
71 FDPS 2224
Affinity Capture-MS Homo sapiens
72 GPR45  
Affinity Capture-MS Homo sapiens
73 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
74 SLC30A7 148867
Affinity Capture-MS Homo sapiens
75 COPB2 9276
Affinity Capture-MS Homo sapiens
76 Jup 16480
Affinity Capture-MS Mus musculus
77 Nedd1  
Affinity Capture-MS Mus musculus
78 ATP5C1 509
Affinity Capture-MS Homo sapiens
79 NANOG  
Affinity Capture-MS Homo sapiens
80 LMNA 4000
Proximity Label-MS Homo sapiens
81 RTN4 57142
Affinity Capture-MS Homo sapiens
82 ANAPC4 29945
Affinity Capture-MS Homo sapiens
83 NUBPL 80224
Affinity Capture-MS Homo sapiens
84 SLC4A2 6522
Affinity Capture-MS Homo sapiens
85 SNCA 6622
Affinity Capture-MS Homo sapiens
86 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
87 ATP5J 522
Affinity Capture-MS Homo sapiens
88 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
89 PGRMC1 10857
Affinity Capture-MS Homo sapiens
90 RTN3 10313
Affinity Capture-MS Homo sapiens
91 MFSD4  
Affinity Capture-MS Homo sapiens
92 Fbl 14113
Affinity Capture-MS Mus musculus
93 TSPAN15 23555
Affinity Capture-MS Homo sapiens
94 GBA 2629
Affinity Capture-MS Homo sapiens
95 EBP  
Affinity Capture-MS Homo sapiens
96 RABL3 285282
Affinity Capture-MS Homo sapiens
97 PTPN5  
Affinity Capture-MS Homo sapiens
98 SPPL2B 56928
Affinity Capture-MS Homo sapiens
99 LPAR2 9170
Affinity Capture-MS Homo sapiens
100 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
101 VIPR1  
Affinity Capture-MS Homo sapiens
102 Ufl1  
Affinity Capture-MS Mus musculus
103 C9orf72  
Affinity Capture-MS Homo sapiens
104 WFS1 7466
Affinity Capture-MS Homo sapiens
105 SLC1A1 6505
Affinity Capture-MS Homo sapiens
106 IKBKB 3551
Affinity Capture-MS Homo sapiens
107 BRI3BP 140707
Affinity Capture-MS Homo sapiens
108 FPR2  
Affinity Capture-MS Homo sapiens
109 DERL1 79139
Proximity Label-MS Homo sapiens
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