Gene description for DHCR24
Gene name 24-dehydrocholesterol reductase
Gene symbol DHCR24
Other names/aliases DCE
Nbla03646
SELADIN1
seladin-1
Species Homo sapiens
 Database cross references - DHCR24
ExoCarta ExoCarta_1718
Entrez Gene 1718
HGNC 2859
MIM 606418
UniProt Q15392  
 DHCR24 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for DHCR24
Molecular Function
    flavin adenine dinucleotide binding GO:0050660 IEA
    delta24(24-1) sterol reductase activity GO:0000246 IMP
    UDP-N-acetylmuramate dehydrogenase activity GO:0008762 IEA
    enzyme binding GO:0019899 IPI
    oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor GO:0016628 IDA
    peptide antigen binding GO:0042605 IPI
    delta24-sterol reductase activity GO:0050614 IEA
Biological Process
    response to hormone GO:0009725 IEA
    regulation of neuron death GO:1901214 NAS
    apoptotic process GO:0006915 NAS
    protein localization GO:0008104 IEA
    cell cycle arrest GO:0007050 NAS
    small molecule metabolic process GO:0044281 TAS
    membrane organization GO:0061024 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    oxidation-reduction process GO:0055114 IMP
    skin development GO:0043588 ISS
    response to oxidative stress GO:0006979 IEP
    amyloid precursor protein catabolic process GO:0042987 IEA
    negative regulation of cell proliferation GO:0008285 IEA
    male genitalia development GO:0030539 IEA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IDA
    cholesterol biosynthetic process GO:0006695 TAS
    tissue development GO:0009888 IMP
    plasminogen activation GO:0031639 IEA
    Ras protein signal transduction GO:0007265 IEA
Subcellular Localization
    cytosol GO:0005829 IEA
    cytoskeleton GO:0005856 IEA
    nucleus GO:0005634 IDA
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum GO:0005783 IDA
    cytoplasm GO:0005737 IBA
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified DHCR24 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DHCR24
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DHCR24 is involved
PathwayEvidenceSource
Cholesterol biosynthesis TAS Reactome





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