Gene description for SLC4A2
Gene name solute carrier family 4 (anion exchanger), member 2
Gene symbol SLC4A2
Other names/aliases AE2
BND3L
EPB3L1
HKB3
NBND3
Species Homo sapiens
 Database cross references - SLC4A2
ExoCarta ExoCarta_6522
Vesiclepedia VP_6522
Entrez Gene 6522
HGNC 11028
MIM 109280
UniProt P04920  
 SLC4A2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC4A2
Molecular Function
    solute:inorganic anion antiporter activity GO:0005452 TAS
    protein binding GO:0005515 IPI
    monoatomic anion transmembrane transporter activity GO:0008509 TAS
    enzyme binding GO:0019899 IEA
    transmembrane transporter activity GO:0022857 IBA
    chloride:bicarbonate antiporter activity GO:0140900 IDA
    chloride:bicarbonate antiporter activity GO:0140900 IEA
    chloride:bicarbonate antiporter activity GO:0140900 IMP
Biological Process
    monoatomic anion transport GO:0006820 TAS
    spermatogenesis GO:0007283 IEA
    bicarbonate transport GO:0015701 IBA
    bicarbonate transport GO:0015701 TAS
    osteoclast differentiation GO:0030316 IMP
    regulation of actin cytoskeleton organization GO:0032956 ISS
    negative regulation of CD8-positive, alpha-beta T cell differentiation GO:0043377 ISS
    regulation of bone resorption GO:0045124 ISS
    digestive tract development GO:0048565 IEA
    regulation of intracellular pH GO:0051453 IBA
    transmembrane transport GO:0055085 IBA
    positive regulation of enamel mineralization GO:0070175 ISS
    amelogenesis GO:0097186 ISS
    chloride transmembrane transport GO:1902476 IEA
    negative regulation of CD8-positive, alpha-beta T cell proliferation GO:2000565 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    membrane GO:0016020 HDA
    basolateral plasma membrane GO:0016323 IBA
    apical plasma membrane GO:0016324 IBA
 Experiment description of studies that identified SLC4A2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
12
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC4A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STARD3NL 83930
Affinity Capture-MS Homo sapiens
2 KDELR3 11015
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 CEMIP 57214
Affinity Capture-MS Homo sapiens
5 Cyr61  
Affinity Capture-MS Mus musculus
6 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 OCLN 100506658
Proximity Label-MS Homo sapiens
8 RXFP1  
Affinity Capture-MS Homo sapiens
9 OR10H2  
Affinity Capture-MS Homo sapiens
10 METTL7A 25840
Proximity Label-MS Homo sapiens
11 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 LGALS9C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 Iqgap3 404710
Affinity Capture-MS Mus musculus
14 THOC2 57187
Affinity Capture-MS Homo sapiens
15 Phospho1  
Affinity Capture-MS Mus musculus
16 LGALS1 3956
Affinity Capture-MS Homo sapiens
17 HSD17B11 51170
Proximity Label-MS Homo sapiens
18 GPR114  
Affinity Capture-MS Homo sapiens
19 STX4 6810
Proximity Label-MS Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 MS4A15  
Affinity Capture-MS Homo sapiens
22 B3GAT1  
Proximity Label-MS Homo sapiens
23 OR6T1  
Affinity Capture-MS Homo sapiens
24 P2RY1  
Affinity Capture-MS Homo sapiens
25 SLC30A2  
Affinity Capture-MS Homo sapiens
26 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
27 C11orf52 91894
Proximity Label-MS Homo sapiens
28 RPN2 6185
Proximity Label-MS Homo sapiens
29 CCDC47 57003
Affinity Capture-MS Homo sapiens
30 F2RL1  
Affinity Capture-MS Homo sapiens
31 LMAN2 10960
Affinity Capture-MS Homo sapiens
32 RAB35 11021
Proximity Label-MS Homo sapiens
33 Kif2c 73804
Affinity Capture-MS Mus musculus
34 KRTCAP2 200185
Affinity Capture-MS Homo sapiens
35 PTH1R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 GPR17 2840
Affinity Capture-MS Homo sapiens
37 LAMTOR1 55004
Proximity Label-MS Homo sapiens
38 KCNK1  
Affinity Capture-MS Homo sapiens
39 C1orf64  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 OR5H1  
Affinity Capture-MS Homo sapiens
41 GJA1 2697
Proximity Label-MS Homo sapiens
42 P2RY8  
Affinity Capture-MS Homo sapiens
43 OR4N2  
Affinity Capture-MS Homo sapiens
44 DNAJC5 80331
Proximity Label-MS Homo sapiens
45 SPDEF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 KCNA6  
Affinity Capture-MS Homo sapiens
47 GPR182  
Affinity Capture-MS Homo sapiens
48 SLC4A8 9498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 MOV10 4343
Affinity Capture-RNA Homo sapiens
50 RPN1 6184
Proximity Label-MS Homo sapiens
51 TMEM63A 9725
Affinity Capture-MS Homo sapiens
52 ELOVL5 60481
Proximity Label-MS Homo sapiens
53 DHCR24 1718
Affinity Capture-MS Homo sapiens
54 NMUR2  
Affinity Capture-MS Homo sapiens
55 ELL2 22936
Affinity Capture-MS Homo sapiens
56 ATXN1 6310
Two-hybrid Homo sapiens
57 P2RY2 5029
Affinity Capture-MS Homo sapiens
58 VIPR2  
Affinity Capture-MS Homo sapiens
59 C4orf32  
Affinity Capture-MS Homo sapiens
60 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 TTYH1  
Affinity Capture-MS Homo sapiens
62 CANX 821
Affinity Capture-MS Homo sapiens
63 RAB7A 7879
Proximity Label-MS Homo sapiens
64 ATL3 25923
Affinity Capture-MS Homo sapiens
65 PANX1 24145
Proximity Label-MS Homo sapiens
66 SLC41A3 54946
Affinity Capture-MS Homo sapiens
67 LAMP1 3916
Proximity Label-MS Homo sapiens
68 CRELD1 78987
Affinity Capture-MS Homo sapiens
69 BCAP31 10134
Proximity Label-MS Homo sapiens
70 HSD3B7 80270
Proximity Label-MS Homo sapiens
71 LGALS9B 284194
Affinity Capture-MS Homo sapiens
72 ARF6 382
Proximity Label-MS Homo sapiens
73 TBXA2R 6915
Affinity Capture-MS Homo sapiens
74 Vps4b 20479
Affinity Capture-MS Mus musculus
75 FAM134C 162427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 ATP2A1 487
Proximity Label-MS Homo sapiens
77 CMTM5  
Affinity Capture-MS Homo sapiens
78 ACKR2  
Affinity Capture-MS Homo sapiens
79 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 VIPR1  
Affinity Capture-MS Homo sapiens
81 CLGN 1047
Affinity Capture-MS Homo sapiens
82 NXF1 10482
Affinity Capture-RNA Homo sapiens
83 GABRE  
Affinity Capture-MS Homo sapiens
84 SIDT2 51092
Affinity Capture-MS Homo sapiens
85 OPALIN  
Affinity Capture-MS Homo sapiens
86 XPR1 9213
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 SLC39A8 64116
Affinity Capture-MS Homo sapiens
89 TSPAN15 23555
Affinity Capture-MS Homo sapiens
90 B4GAT1 11041
Affinity Capture-MS Homo sapiens
91 DDX58 23586
Affinity Capture-RNA Homo sapiens
92 REP15  
Affinity Capture-MS Homo sapiens
93 Myh10 77579
Affinity Capture-MS Mus musculus
94 ESR1  
Affinity Capture-MS Homo sapiens
95 GPR52  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 RAB2A 5862
Proximity Label-MS Homo sapiens
97 SEC61B 10952
Proximity Label-MS Homo sapiens
98 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 OR52I1  
Affinity Capture-MS Homo sapiens
100 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 LPAR2 9170
Affinity Capture-MS Homo sapiens
102 TMC4  
Affinity Capture-MS Homo sapiens
103 DNAJC1 64215
Proximity Label-MS Homo sapiens
104 KRAS 3845
Proximity Label-MS Homo sapiens
105 RAB5C 5878
Proximity Label-MS Homo sapiens
106 ADGRE5 976
Affinity Capture-MS Homo sapiens
107 NKAIN1  
Affinity Capture-MS Homo sapiens
108 CCR6  
Affinity Capture-MS Homo sapiens
109 CAV1 857
Proximity Label-MS Homo sapiens
110 S1PR4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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