Gene description for ELL2
Gene name elongation factor, RNA polymerase II, 2
Gene symbol ELL2
Other names/aliases -
Species Homo sapiens
 Database cross references - ELL2
ExoCarta ExoCarta_22936
Vesiclepedia VP_22936
Entrez Gene 22936
HGNC 17064
MIM 601874
UniProt O00472  
 ELL2 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ELL2
Molecular Function
    cis-regulatory region sequence-specific DNA binding GO:0000987 IBA
    protein binding GO:0005515 IPI
Biological Process
    transcription elongation by RNA polymerase II GO:0006368 IEA
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IBA
    snRNA transcription by RNA polymerase II GO:0042795 IBA
    snRNA transcription by RNA polymerase II GO:0042795 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription elongation factor complex GO:0008023 IBA
    transcription elongation factor complex GO:0008023 IDA
 Experiment description of studies that identified ELL2 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ELL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 SLC4A2 6522
Affinity Capture-MS Homo sapiens
3 AFG3L2 10939
Affinity Capture-MS Homo sapiens
4 Mcm3 17215
Affinity Capture-MS Mus musculus
5 KIF23 9493
Affinity Capture-MS Homo sapiens
6 ESYT2 57488
Affinity Capture-MS Homo sapiens
7 MLLT1 4298
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PRKAB2 5565
Two-hybrid Homo sapiens
9 TIA1 7072
Affinity Capture-MS Homo sapiens
10 NCSTN 23385
Affinity Capture-MS Homo sapiens
11 EAF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
12 EXOC8 149371
Two-hybrid Homo sapiens
13 RNF181  
Two-hybrid Homo sapiens
14 CASK 8573
Two-hybrid Homo sapiens
15 EFR3A 23167
Affinity Capture-MS Homo sapiens
16 EAF2  
Affinity Capture-Western Homo sapiens
17 ELL  
Negative Genetic Homo sapiens
18 PTEN 5728
Negative Genetic Homo sapiens
19 AFF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
20 DDX58 23586
Affinity Capture-RNA Homo sapiens
21 OFD1 8481
Affinity Capture-MS Homo sapiens
22 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 AQP2 359
Two-hybrid Homo sapiens
24 AFF4 27125
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CDK9 1025
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
26 MLLT3 4300
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
27 Mllt1  
Affinity Capture-MS Mus musculus
28 NBEAL2 23218
Affinity Capture-MS Homo sapiens
29 CTR9 9646
Affinity Capture-MS Homo sapiens
30 Ppp2r5c 26931
Affinity Capture-MS Mus musculus
31 Aff1  
Affinity Capture-MS Mus musculus
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