Gene description for AFF4
Gene name AF4/FMR2 family, member 4
Gene symbol AFF4
Other names/aliases AF5Q31
MCEF
Species Homo sapiens
 Database cross references - AFF4
ExoCarta ExoCarta_27125
Vesiclepedia VP_27125
Entrez Gene 27125
HGNC 17869
MIM 604417
UniProt Q9UHB7  
 AFF4 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for AFF4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    spermatid development GO:0007286 IEA
    regulation of gene expression GO:0010468 IBA
    response to endoplasmic reticulum stress GO:0034976 IEA
Subcellular Localization
    euchromatin GO:0000791 ISS
    fibrillar center GO:0001650 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription elongation factor complex GO:0008023 IDA
    super elongation complex GO:0032783 IBA
 Experiment description of studies that identified AFF4 in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for AFF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 CD3EAP  
Proximity Label-MS Homo sapiens
3 ELL2 22936
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PLCD4  
Affinity Capture-MS Homo sapiens
6 GRK5 2869
Affinity Capture-MS Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 ARRB2 409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 EPB41L1 2036
Affinity Capture-MS Homo sapiens
10 AP2B1 163
Two-hybrid Homo sapiens
11 PBX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MLLT1 4298
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
13 USP7 7874
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 TRAF2 7186
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 PARP1 142
Proximity Label-MS Homo sapiens
16 GRK6 2870
Affinity Capture-MS Homo sapiens
17 COIL  
Proximity Label-MS Homo sapiens
18 KMT2A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
19 EAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 KIF14 9928
Affinity Capture-MS Homo sapiens
21 PES1 23481
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MDK 4192
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
24 MEN1 4221
Affinity Capture-MS Homo sapiens
25 SPP1 6696
Two-hybrid Homo sapiens
26 EPB41L3 23136
Affinity Capture-MS Homo sapiens
27 FGF12  
Affinity Capture-MS Homo sapiens
28 APEX1 328
Proximity Label-MS Homo sapiens
29 SYT2  
Affinity Capture-MS Homo sapiens
30 EPB41L5 57669
Affinity Capture-MS Homo sapiens
31 NFKBIL1  
Affinity Capture-MS Homo sapiens
32 NOP56 10528
Proximity Label-MS Homo sapiens
33 DHX8 1659
Proximity Label-MS Homo sapiens
34 CDK15  
Affinity Capture-MS Homo sapiens
35 RSBN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 AFF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
37 SMOC1 64093
Affinity Capture-MS Homo sapiens
38 ELL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 NIFK 84365
Proximity Label-MS Homo sapiens
40 GOLGA2 2801
Two-hybrid Homo sapiens
41 TRIM24  
Affinity Capture-MS Homo sapiens
42 TCEB3 6924
Affinity Capture-MS Homo sapiens
43 ELL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 DDX23 9416
Proximity Label-MS Homo sapiens
45 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 UBR5 51366
Co-fractionation Homo sapiens
48 LRRTM4  
Affinity Capture-MS Homo sapiens
49 CNOT1 23019
Affinity Capture-MS Homo sapiens
50 FAM9A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 DENND2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 KIF23 9493
Affinity Capture-MS Homo sapiens
53 CDK9 1025
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RNF151  
Affinity Capture-MS Homo sapiens
55 SP110  
Affinity Capture-MS Homo sapiens
56 PLCD3 113026
Affinity Capture-MS Homo sapiens
57 AR 367
Proximity Label-MS Homo sapiens
58 MLLT3 4300
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
59 FAM133A 286499
Affinity Capture-MS Homo sapiens
60 Mllt1  
Affinity Capture-MS Mus musculus
61 LYAR 55646
Affinity Capture-MS Homo sapiens
62 JMY  
Affinity Capture-MS Homo sapiens
63 HEXIM1 10614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RXRB 6257
Affinity Capture-MS Homo sapiens
65 EAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CTR9 9646
Affinity Capture-MS Homo sapiens
67 CCNT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 MTUS2 23281
Two-hybrid Homo sapiens
69 HECW2  
Affinity Capture-MS Homo sapiens
70 Aff1  
Affinity Capture-MS Mus musculus
71 RASA2 5922
Affinity Capture-MS Homo sapiens
72 DAXX  
Affinity Capture-MS Homo sapiens
73 CCDC8  
Affinity Capture-MS Homo sapiens
74 CT45A5  
Affinity Capture-MS Homo sapiens
75 CCNT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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