Gene description for RASA2
Gene name RAS p21 protein activator 2
Gene symbol RASA2
Other names/aliases GAP1M
Species Homo sapiens
 Database cross references - RASA2
ExoCarta ExoCarta_5922
Entrez Gene 5922
HGNC 9872
MIM 601589
UniProt Q15283  
 RASA2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for RASA2
Molecular Function
    metal ion binding GO:0046872 IEA
    GTPase activator activity GO:0005096 IEA
Biological Process
    negative regulation of Ras protein signal transduction GO:0046580 IBA
    intracellular signal transduction GO:0035556 IEA
    positive regulation of GTPase activity GO:0043547 TAS
    signal transduction GO:0007165 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    intrinsic component of the cytoplasmic side of the plasma membrane GO:0031235 IBA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified RASA2 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RASA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNA12 2768
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which RASA2 is involved
No pathways found





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