Gene description for GAPVD1
Gene name GTPase activating protein and VPS9 domains 1
Gene symbol GAPVD1
Other names/aliases GAPEX5
GAPex-5
RAP6
Species Homo sapiens
 Database cross references - GAPVD1
ExoCarta ExoCarta_26130
Vesiclepedia VP_26130
Entrez Gene 26130
HGNC 23375
MIM 611714
UniProt Q14C86  
 GAPVD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GAPVD1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IBA
    GTPase activating protein binding GO:0032794 IMP
    cadherin binding GO:0045296 HDA
Biological Process
    endocytosis GO:0006897 IEA
    regulation of protein transport GO:0051223 IMP
Subcellular Localization
    endosome GO:0005768 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    endocytic vesicle GO:0030139 IBA
 Experiment description of studies that identified GAPVD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GAPVD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Csnk1d  
Affinity Capture-MS Mus musculus
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 FOXF1 2294
Affinity Capture-MS Homo sapiens
4 SNX24  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PSMD11 5717
Co-fractionation Homo sapiens
6 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ACTC1 70
Proximity Label-MS Homo sapiens
8 CALM1 801
Co-fractionation Homo sapiens
9 TROVE2 6738
Co-fractionation Homo sapiens
10 HNRNPR 10236
Co-fractionation Homo sapiens
11 EPS15L1 58513
Affinity Capture-MS Homo sapiens
12 ZNF618  
Affinity Capture-MS Homo sapiens
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 YWHAZ 7534
Affinity Capture-MS Homo sapiens
15 RASA2 5922
Co-fractionation Homo sapiens
16 HSPA2 3306
Affinity Capture-MS Homo sapiens
17 MCM2 4171
Affinity Capture-MS Homo sapiens
18 Tube1  
Affinity Capture-MS Mus musculus
19 GNS 2799
Co-fractionation Homo sapiens
20 SPAG9 9043
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
21 IQGAP1 8826
Co-fractionation Homo sapiens
22 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 SMC2 10592
Co-fractionation Homo sapiens
24 MCM6 4175
Affinity Capture-MS Homo sapiens
25 PSMC2 5701
Co-fractionation Homo sapiens
26 MED17  
Affinity Capture-MS Homo sapiens
27 FBXO11  
Affinity Capture-MS Homo sapiens
28 TSSK1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 YWHAH 7533
Affinity Capture-MS Homo sapiens
30 RAB35 11021
Proximity Label-MS Homo sapiens
31 CPT2 1376
Co-fractionation Homo sapiens
32 CSNK1D 1453
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 NPAS1  
Affinity Capture-MS Homo sapiens
34 PSMC3 5702
Co-fractionation Homo sapiens
35 KIF14 9928
Affinity Capture-MS Homo sapiens
36 CBL 867
Affinity Capture-Western Homo sapiens
37 HSPA8 3312
Co-fractionation Homo sapiens
38 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
39 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 MCC 4163
Affinity Capture-MS Homo sapiens
41 CCDC6 8030
Co-fractionation Homo sapiens
42 TGM2 7052
Co-fractionation Homo sapiens
43 PARK2  
Affinity Capture-MS Homo sapiens
44 THRSP  
Affinity Capture-MS Homo sapiens
45 MICU2 221154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CDH1 999
Proximity Label-MS Homo sapiens
47 YWHAE 7531
Affinity Capture-MS Homo sapiens
48 MYH9 4627
Co-fractionation Homo sapiens
49 TRIM21 6737
Affinity Capture-MS Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 CSNK1E 1454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MYH10 4628
Co-fractionation Homo sapiens
53 VAV2 7410
Two-hybrid Homo sapiens
54 RAD21 5885
Affinity Capture-Western Homo sapiens
55 USP32 84669
Affinity Capture-MS Homo sapiens
56 HSPA5 3309
Co-fractionation Homo sapiens
57 PER2  
Affinity Capture-MS Homo sapiens
58 COPS2 9318
Co-fractionation Homo sapiens
59 C1orf111  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 UFC1 51506
Cross-Linking-MS (XL-MS) Homo sapiens
61 AHCYL1 10768
Co-fractionation Homo sapiens
62 XPO1 7514
Affinity Capture-MS Homo sapiens
63 USP15 9958
Affinity Capture-MS Homo sapiens
64 BAG2 9532
Affinity Capture-MS Homo sapiens
65 SIRT7  
Affinity Capture-MS Homo sapiens
66 RASAL1 8437
Co-fractionation Homo sapiens
67 GGH 8836
Affinity Capture-MS Homo sapiens
68 PRKAR2A 5576
Co-fractionation Homo sapiens
69 CUL3 8452
Affinity Capture-MS Homo sapiens
70 NR3C1 2908
Co-fractionation Homo sapiens
71 LRRC47 57470
Co-fractionation Homo sapiens
72 MCM4 4173
Affinity Capture-MS Homo sapiens
73 FOXL1  
Affinity Capture-MS Homo sapiens
74 METTL21B  
Affinity Capture-MS Homo sapiens
75 GTF3C4 9329
Affinity Capture-MS Homo sapiens
76 NXF1 10482
Affinity Capture-RNA Homo sapiens
77 DUSP13  
Affinity Capture-MS Homo sapiens
78 Csnk1e  
Affinity Capture-MS Mus musculus
79 FGFR2 2263
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 KCNAB2 8514
Affinity Capture-MS Homo sapiens
81 GOLGA1  
Proximity Label-MS Homo sapiens
82 TXNL1 9352
Co-fractionation Homo sapiens
83 HSPA1A 3303
Affinity Capture-MS Homo sapiens
84 HSPA9 3313
Co-fractionation Homo sapiens
85 HOOK3 84376
Proximity Label-MS Homo sapiens
86 FOXK1 221937
Co-fractionation Homo sapiens
87 CCDC8  
Affinity Capture-MS Homo sapiens
88 RNF43  
Proximity Label-MS Homo sapiens
89 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
90 PRKCSH 5589
Cross-Linking-MS (XL-MS) Homo sapiens
91 SIRT6  
Affinity Capture-MS Homo sapiens
92 PARP1 142
Proximity Label-MS Homo sapiens
93 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
94 SF3A1 10291
Co-fractionation Homo sapiens
95 FOXB1  
Affinity Capture-MS Homo sapiens
96 LRPPRC 10128
Co-fractionation Homo sapiens
97 HSPB1 3315
Two-hybrid Homo sapiens
98 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
99 RASA3 22821
Co-fractionation Homo sapiens
100 PRMT5 10419
Co-fractionation Homo sapiens
101 AXIN1  
Affinity Capture-MS Homo sapiens
102 FAM110D  
Affinity Capture-MS Homo sapiens
103 CAD 790
Co-fractionation Homo sapiens
104 HSF1  
Affinity Capture-MS Homo sapiens
105 SERBP1 26135
Affinity Capture-MS Homo sapiens
106 IVNS1ABP  
Affinity Capture-MS Homo sapiens
107 PSMD3 5709
Co-fractionation Homo sapiens
View the network image/svg+xml



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