Gene description for PRKAR2A
Gene name protein kinase, cAMP-dependent, regulatory, type II, alpha
Gene symbol PRKAR2A
Other names/aliases PKR2
PRKAR2
Species Homo sapiens
 Database cross references - PRKAR2A
ExoCarta ExoCarta_5576
Entrez Gene 5576
HGNC 9391
MIM 176910
UniProt P13861  
 PRKAR2A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for PRKAR2A
Molecular Function
    cAMP binding GO:0030552 IEA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IDA
    protein binding GO:0005515 IPI
    protein kinase A catalytic subunit binding GO:0034236 IPI
    cAMP-dependent protein kinase regulator activity GO:0008603 NAS
    ubiquitin protein ligase binding GO:0031625 IDA
Biological Process
    regulation of insulin secretion GO:0050796 TAS
    activation of protein kinase A activity GO:0034199 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    cellular response to glucagon stimulus GO:0071377 TAS
    blood coagulation GO:0007596 TAS
    intracellular signal transduction GO:0035556 TAS
    transmembrane transport GO:0055085 TAS
    renal water homeostasis GO:0003091 TAS
    small molecule metabolic process GO:0044281 TAS
    negative regulation of cAMP-dependent protein kinase activity GO:2000480 IDA
    activation of phospholipase C activity GO:0007202 TAS
    energy reserve metabolic process GO:0006112 TAS
    water transport GO:0006833 TAS
    signal transduction GO:0007165 TAS
    innate immune response GO:0045087 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
Subcellular Localization
    membrane GO:0016020 IDA
    ciliary base GO:0097546 TAS
    extracellular exosome GO:0070062 IDA
    AMP-activated protein kinase complex GO:0031588 IDA
    focal adhesion GO:0005925 IDA
    plasma membrane GO:0005886 IDA
    cytosol GO:0005829 TAS
    cAMP-dependent protein kinase complex GO:0005952 IEA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
 Experiment description of studies that identified PRKAR2A in exosomes
1
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PRKAR2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARFGEF2 10564
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 CBFA2T3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 GSK3B 2932
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 AKAP14  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 RYR2 6262
Co-fractionation Homo sapiens
6 AKAP9 10142
Invivo Homo sapiens
Invitro Homo sapiens
Protein-peptide Homo sapiens
7 EZR 7430
Affinity Capture-MS Homo sapiens
8 AKAP12 9590
Affinity Capture-Western Homo sapiens
9 AKAP7  
Protein-peptide Homo sapiens
10 AKAP13 11214
Invitro Homo sapiens
Protein-peptide Homo sapiens
11 NBEA  
Invitro Homo sapiens
Invivo Homo sapiens
12 PDE4A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 KCNQ1  
Affinity Capture-Western Homo sapiens
14 MYCBPAP 84073
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
15 RAB32 10981
Far Western Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
16 PALM2-AKAP2  
Invivo Homo sapiens
Invitro Homo sapiens
17 AKAP8  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 AKAP3  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
19 RUNX1T1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
20 AKAP6  
Invivo Homo sapiens
Invitro Homo sapiens
Protein-peptide Homo sapiens
21 AKAP5  
Protein-peptide Homo sapiens
22 AKAP11 11215
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
23 AKAP1  
Reconstituted Complex Homo sapiens
24 PRKX 5613
Invitro Homo sapiens
25 GNA13 10672
Affinity Capture-Western Homo sapiens
26 AKAP2 11217
Invivo Homo sapiens
Invitro Homo sapiens
Protein-peptide Homo sapiens
27 AKAP10  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
28 SCTR  
Invitro Homo sapiens
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