Gene description for Prkar2b
Gene name protein kinase, cAMP dependent regulatory, type II beta
Gene symbol Prkar2b
Other names/aliases AI451071
AW061005
PKARIIbeta
Pkarb2
RII(beta)
Species Mus musculus
 Database cross references - Prkar2b
ExoCarta ExoCarta_19088
Vesiclepedia VP_19088
Entrez Gene 19088
UniProt P31324  
 Prkar2b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prkar2b
Molecular Function
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IBA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IEA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    protein binding GO:0005515 IPI
    cAMP-dependent protein kinase regulator activity GO:0008603 IDA
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
    protein kinase binding GO:0019901 IPI
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    cAMP binding GO:0030552 IBA
    cAMP binding GO:0030552 IEA
    cAMP binding GO:0030552 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein kinase A catalytic subunit binding GO:0034236 IBA
    protein kinase A catalytic subunit binding GO:0034236 IEA
    protein kinase A catalytic subunit binding GO:0034236 ISO
Biological Process
    fatty acid metabolic process GO:0006631 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    learning GO:0007612 IMP
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    response to antipsychotic drug GO:0097332 IEA
    negative regulation of cAMP/PKA signal transduction GO:0141162 IEA
    negative regulation of cAMP/PKA signal transduction GO:0141162 ISO
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cAMP-dependent protein kinase complex GO:0005952 IBA
    cAMP-dependent protein kinase complex GO:0005952 IDA
    cAMP-dependent protein kinase complex GO:0005952 ISO
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 IDA
    dendritic shaft GO:0043198 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    ciliary base GO:0097546 IDA
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IMP
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Prkar2b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prkar2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
2 Tnik  
Affinity Capture-MS Mus musculus
3 CDK5RAP2 55755
Affinity Capture-MS Homo sapiens
4 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
5 Grin2b  
Affinity Capture-Western Mus musculus
6 PRKACA 5566
Affinity Capture-MS Homo sapiens
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Pde4dip  
Affinity Capture-MS Mus musculus
9 Dlg4  
Affinity Capture-MS Mus musculus
10 Atxn1  
Affinity Capture-MS Mus musculus
11 Agap2  
Affinity Capture-MS Mus musculus
12 Uso1 56041
Co-fractionation Mus musculus
13 PRKACB  
Affinity Capture-MS Bos taurus
14 PRKACB 5567
Affinity Capture-MS Homo sapiens
15 AKAP9 10142
Affinity Capture-MS Homo sapiens
16 Grin1  
Affinity Capture-Western Mus musculus
17 Ywhaz 22631
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Prkar2b is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here