Gene description for Uso1
Gene name USO1 vesicle docking factor
Gene symbol Uso1
Other names/aliases 115kDa
TAP
Vdp
Species Mus musculus
 Database cross references - Uso1
ExoCarta ExoCarta_56041
Vesiclepedia VP_56041
Entrez Gene 56041
UniProt Q9Z1Z0  
 Uso1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Uso1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISO
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISS
    Golgi organization GO:0007030 IMP
    small GTPase-mediated signal transduction GO:0007264 IDA
    secretory granule localization GO:0032252 IDA
    transcytosis GO:0045056 IBA
    transcytosis GO:0045056 ISO
    Golgi vesicle docking GO:0048211 IBA
    vesicle fusion with Golgi apparatus GO:0048280 IEA
    membrane fusion GO:0061025 IBA
    membrane fusion GO:0061025 ISO
    regulation of cellular response to insulin stimulus GO:1900076 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    endoplasmic reticulum GO:0005783 IBA
    Golgi apparatus GO:0005794 ISO
    Golgi stack GO:0005795 IBA
    cytosol GO:0005829 IDA
    ER to Golgi transport vesicle membrane GO:0012507 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified Uso1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Uso1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 PDCD6 10016
Affinity Capture-MS Homo sapiens
3 AP2B1 163
Affinity Capture-MS Homo sapiens
4 NCBP1 4686
Affinity Capture-MS Homo sapiens
5 Dctn1 13191
Co-fractionation Mus musculus
6 ARPC2 10109
Affinity Capture-MS Homo sapiens
7 PKP2 5318
Affinity Capture-MS Homo sapiens
8 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
9 MAN1B1 11253
Affinity Capture-MS Homo sapiens
10 SURF4 6836
Affinity Capture-MS Homo sapiens
11 GOSR2 9570
Affinity Capture-MS Homo sapiens
12 CLTB 1212
Affinity Capture-MS Homo sapiens
13 UBXN2A  
Affinity Capture-MS Homo sapiens
14 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
15 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
16 TNK1  
Affinity Capture-MS Homo sapiens
17 SPECC1L 23384
Affinity Capture-MS Homo sapiens
18 FAM83B  
Affinity Capture-MS Homo sapiens
19 AP2A1 160
Affinity Capture-MS Homo sapiens
20 SEC24C 9632
Affinity Capture-MS Homo sapiens
21 TM9SF4 9777
Affinity Capture-MS Homo sapiens
22 NCOA5  
Affinity Capture-MS Homo sapiens
23 Golga2  
Co-fractionation Mus musculus
24 YIPF3 25844
Affinity Capture-MS Homo sapiens
25 Lnpep 240028
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Reconstituted Complex Mus musculus
Co-fractionation Mus musculus
26 PCM1 5108
Affinity Capture-MS Homo sapiens
27 NAP1L4 4676
Affinity Capture-MS Homo sapiens
28 GTSE1 51512
Affinity Capture-MS Homo sapiens
29 GORASP1 64689
Affinity Capture-MS Homo sapiens
30 CEP131 22994
Affinity Capture-MS Homo sapiens
31 MPRIP 23164
Affinity Capture-MS Homo sapiens
32 RBM14 10432
Affinity Capture-MS Homo sapiens
33 AMBRA1  
Affinity Capture-MS Homo sapiens
34 GOLIM4 27333
Affinity Capture-MS Homo sapiens
35 UBR4 23352
Affinity Capture-MS Homo sapiens
36 AP3M1 26985
Affinity Capture-MS Homo sapiens
37 Foxp3  
Affinity Capture-MS Mus musculus
38 SCFD1 23256
Affinity Capture-MS Homo sapiens
39 P4HA1 5033
Affinity Capture-MS Homo sapiens
40 WNK1 65125
Affinity Capture-MS Homo sapiens
41 TMOD1  
Affinity Capture-MS Homo sapiens
42 TUBA1A 7846
Affinity Capture-MS Homo sapiens
43 CLTCL1 8218
Affinity Capture-MS Homo sapiens
44 GOLGA2 2801
Affinity Capture-MS Homo sapiens
45 SRRT 51593
Affinity Capture-MS Homo sapiens
46 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
47 ABCF2 10061
Affinity Capture-MS Homo sapiens
48 CEP170 9859
Affinity Capture-MS Homo sapiens
49 ACTL8  
Affinity Capture-MS Homo sapiens
50 BTBD3  
Affinity Capture-MS Homo sapiens
51 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
52 GORASP2 26003
Affinity Capture-MS Homo sapiens
53 BMP2K 55589
Affinity Capture-MS Homo sapiens
54 CSNK1E 1454
Affinity Capture-MS Homo sapiens
55 STX5 6811
Affinity Capture-MS Homo sapiens
56 PIK3C2A 5286
Affinity Capture-MS Homo sapiens
57 CEP72  
Affinity Capture-MS Homo sapiens
58 ATP2C1 27032
Affinity Capture-MS Homo sapiens
59 YIF1A 10897
Affinity Capture-MS Homo sapiens
60 GAK 2580
Affinity Capture-MS Homo sapiens
61 PRKACB 5567
Affinity Capture-MS Homo sapiens
62 YIF1B 90522
Affinity Capture-MS Homo sapiens
63 BCLAF1 9774
Affinity Capture-MS Homo sapiens
64 STON2 85439
Affinity Capture-MS Homo sapiens
65 RBBP6 5930
Affinity Capture-MS Homo sapiens
66 GORAB 92344
Affinity Capture-MS Homo sapiens
67 GOLGA3 2802
Affinity Capture-MS Homo sapiens
68 CLINT1 9685
Affinity Capture-MS Homo sapiens
69 ZFPL1 7542
Affinity Capture-MS Homo sapiens
70 SEC13 6396
Affinity Capture-MS Homo sapiens
71 AKAP9 10142
Affinity Capture-MS Homo sapiens
72 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
73 SLC30A6 55676
Affinity Capture-MS Homo sapiens
74 GOLT1B 51026
Affinity Capture-MS Homo sapiens
75 SLC35E1 79939
Affinity Capture-MS Homo sapiens
76 ENDOD1 23052
Affinity Capture-MS Homo sapiens
77 YIPF5 81555
Affinity Capture-MS Homo sapiens
78 CDK5RAP2 55755
Affinity Capture-MS Homo sapiens
79 C4A 720
Affinity Capture-MS Homo sapiens
80 LZTS2 84445
Affinity Capture-MS Homo sapiens
81 PRKACA 5566
Affinity Capture-MS Homo sapiens
82 TPM1 7168
Affinity Capture-MS Homo sapiens
83 GNB2 2783
Affinity Capture-MS Homo sapiens
84 SEC16A 9919
Affinity Capture-MS Homo sapiens
85 TMOD3 29766
Affinity Capture-MS Homo sapiens
86 FBRS 64319
Affinity Capture-MS Homo sapiens
87 GALNT2 2590
Affinity Capture-MS Homo sapiens
88 CLTC 1213
Affinity Capture-MS Homo sapiens
89 BMP1 649
Affinity Capture-MS Homo sapiens
90 TPM4 7171
Affinity Capture-MS Homo sapiens
91 FARP1 10160
Affinity Capture-MS Homo sapiens
92 Fancd2  
Affinity Capture-MS Mus musculus
93 FAM120A 23196
Affinity Capture-MS Homo sapiens
94 SEC24B 10427
Affinity Capture-MS Homo sapiens
95 NPHP3 27031
Affinity Capture-MS Homo sapiens
96 TNK2 10188
Affinity Capture-MS Homo sapiens
97 TMED10 10972
Affinity Capture-MS Homo sapiens
98 Csnk2b 13001
Co-fractionation Mus musculus
99 Eed  
Affinity Capture-MS Mus musculus
100 CEP68 23177
Affinity Capture-MS Homo sapiens
101 GOSR1 9527
Affinity Capture-MS Homo sapiens
102 YTHDC1  
Affinity Capture-MS Homo sapiens
103 Atxn1  
Affinity Capture-MS Mus musculus
104 Kctd13  
Affinity Capture-MS Mus musculus
105 Chaf1a  
Affinity Capture-MS Mus musculus
106 RNF20 56254
Affinity Capture-MS Homo sapiens
107 FAM83H 286077
Affinity Capture-MS Homo sapiens
108 Dctn2 69654
Co-fractionation Mus musculus
109 RABL3 285282
Affinity Capture-MS Homo sapiens
110 ZNF106  
Affinity Capture-MS Homo sapiens
111 INF2 64423
Affinity Capture-MS Homo sapiens
112 Prkar2b 19088
Co-fractionation Mus musculus
113 CLTA 1211
Affinity Capture-MS Homo sapiens
114 TMED7 51014
Affinity Capture-MS Homo sapiens
115 BLZF1  
Affinity Capture-MS Homo sapiens
116 UBIAD1 29914
Affinity Capture-MS Homo sapiens
117 Eea1  
Co-fractionation Mus musculus
View the network image/svg+xml



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