Gene description for NPHP3
Gene name nephronophthisis 3 (adolescent)
Gene symbol NPHP3
Other names/aliases CFAP31
MKS7
NPH3
RHPD
RHPD1
SLSN3
Species Homo sapiens
 Database cross references - NPHP3
ExoCarta ExoCarta_27031
Vesiclepedia VP_27031
Entrez Gene 27031
HGNC 7907
MIM 608002
UniProt Q7Z494  
 NPHP3 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NPHP3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    kidney development GO:0001822 IBA
    kidney development GO:0001822 IMP
    heart looping GO:0001947 IMP
    atrial septum development GO:0003283 IMP
    lipid metabolic process GO:0006629 IEA
    establishment or maintenance of cell polarity GO:0007163 IEA
    determination of left/right symmetry GO:0007368 IBA
    determination of left/right symmetry GO:0007368 IMP
    Wnt signaling pathway GO:0016055 IEA
    extracellular matrix organization GO:0030198 IEA
    lung development GO:0030324 IMP
    determination of pancreatic left/right asymmetry GO:0035469 IMP
    photoreceptor cell maintenance GO:0045494 IMP
    maintenance of animal organ identity GO:0048496 IMP
    convergent extension GO:0060026 IBA
    convergent extension involved in gastrulation GO:0060027 IGI
    cilium assembly GO:0060271 ISS
    epithelial cilium movement involved in determination of left/right asymmetry GO:0060287 IC
    kidney morphogenesis GO:0060993 IBA
    kidney morphogenesis GO:0060993 IMP
    determination of intestine left/right asymmetry GO:0071908 IMP
    determination of stomach left/right asymmetry GO:0071909 IMP
    determination of liver left/right asymmetry GO:0071910 IMP
    ureter development GO:0072189 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IDA
    non-motile cilium assembly GO:1905515 IEA
    regulation of Wnt signaling pathway, planar cell polarity pathway GO:2000095 IDA
    regulation of Wnt signaling pathway, planar cell polarity pathway GO:2000095 ISS
Subcellular Localization
    extracellular region GO:0005576 IEA
    cytosol GO:0005829 TAS
    cilium GO:0005929 IDA
    cilium GO:0005929 TAS
    ciliary inversin compartment GO:0097543 IBA
    ciliary base GO:0097546 IBA
 Experiment description of studies that identified NPHP3 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NPHP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATXN1 6310
Two-hybrid Homo sapiens
2 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
3 GMNN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GMDS 2762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 FAM167A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 NUP62CL  
Affinity Capture-MS Homo sapiens
7 PIP 5304
Affinity Capture-MS Homo sapiens
8 PALD1 27143
Affinity Capture-MS Homo sapiens
9 RSPRY1 89970
Affinity Capture-MS Homo sapiens
10 GATS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 KLHL29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 C4orf26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NPHP1  
Affinity Capture-Western Homo sapiens
14 Ncapg2  
Affinity Capture-MS Mus musculus
15 BAP1 8314
Affinity Capture-MS Homo sapiens
16 DDX58 23586
Affinity Capture-RNA Homo sapiens
17 PDE9A  
Affinity Capture-MS Homo sapiens
18 AARSD1 80755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ARHGEF35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ARL3 403
Affinity Capture-MS Homo sapiens
21 Uso1 56041
Affinity Capture-MS Mus musculus
22 OGT 8473
Affinity Capture-MS Homo sapiens
23 SNX11 29916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ZNF517  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CEP164  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 CREB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TXNIP 10628
Affinity Capture-MS Homo sapiens
28 UNC119 9094
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 C12orf75  
Affinity Capture-MS Homo sapiens
30 PRKG2  
Affinity Capture-MS Homo sapiens
31 FMNL2 114793
Affinity Capture-MS Homo sapiens
32 PDE8A 5151
Affinity Capture-MS Homo sapiens
33 UNC119B 84747
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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