Gene description for GORASP1
Gene name golgi reassembly stacking protein 1, 65kDa
Gene symbol GORASP1
Other names/aliases GOLPH5
GRASP65
P65
Species Homo sapiens
 Database cross references - GORASP1
ExoCarta ExoCarta_64689
Vesiclepedia VP_64689
Entrez Gene 64689
HGNC 16769
MIM 606867
UniProt Q9BQQ3  
 GORASP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GORASP1
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    protein N-linked glycosylation GO:0006487 IEA
    Golgi organization GO:0007030 IBA
    Golgi organization GO:0007030 ISS
    Golgi organization GO:0007030 NAS
    protein transport GO:0015031 IEA
    negative regulation of dendrite morphogenesis GO:0050774 IMP
    establishment of protein localization to plasma membrane GO:0061951 IMP
    Golgi ribbon formation GO:0090161 IGI
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    cis-Golgi network GO:0005801 NAS
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 TAS
 Experiment description of studies that identified GORASP1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GORASP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 INTS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ATP6V1B2 526
Proximity Label-MS Homo sapiens
4 CLCN7 1186
Proximity Label-MS Homo sapiens
5 STK25 10494
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CLIP1 6249
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SLC15A4 121260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TEX35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RTCA 8634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SNX6 58533
Proximity Label-MS Homo sapiens
11 APEX1 328
Affinity Capture-RNA Homo sapiens
12 WDR48 57599
Affinity Capture-MS Homo sapiens
13 USO1 8615
Proximity Label-MS Homo sapiens
14 MON2 23041
Proximity Label-MS Homo sapiens
15 CASP3 836
Biochemical Activity Homo sapiens
16 HARS2 23438
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TMED2 10959
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
18 STX7 8417
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TDP2 51567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FBXW8 26259
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 NXN 64359
Affinity Capture-MS Homo sapiens
22 TMTC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GUCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NIF3L1 60491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TES 26136
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 KIF14 9928
Proximity Label-MS Homo sapiens
28 CLASP2 23122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 LRRC59 55379
Proximity Label-MS Homo sapiens
30 LONRF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 ALDH1A3 220
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CCNJ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 USP12 219333
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RUFY1 80230
Proximity Label-MS Homo sapiens
35 PPP6R2 9701
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CENPL  
Affinity Capture-MS Homo sapiens
37 Gorasp1  
Affinity Capture-MS Mus musculus
38 GOLGA2 2801
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PDXDC1 23042
Proximity Label-MS Homo sapiens
40 GCC2 9648
Proximity Label-MS Homo sapiens
41 CDKAL1  
Proximity Label-MS Homo sapiens
42 GORASP2 26003
Proximity Label-MS Homo sapiens
43 PLK1 5347
Biochemical Activity Homo sapiens
44 KIAA0391  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TPRKB 51002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 STX5 6811
Proximity Label-MS Homo sapiens
47 ARL13B 200894
Proximity Label-MS Homo sapiens
48 DGCR2 9993
Affinity Capture-MS Homo sapiens
49 SLX4IP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 AGPS 8540
Two-hybrid Homo sapiens
51 FLOT2 2319
Proximity Label-MS Homo sapiens
52 SND1 27044
Proximity Label-MS Homo sapiens
53 ACBD3 64746
Proximity Label-MS Homo sapiens
54 TMF1 7110
Proximity Label-MS Homo sapiens
55 DKK3 27122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CENPN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SCRN1 9805
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 SMCR8 140775
Proximity Label-MS Homo sapiens
59 GOLGA3 2802
Proximity Label-MS Homo sapiens
60 KIF15 56992
Proximity Label-MS Homo sapiens
61 XPO1 7514
Affinity Capture-MS Homo sapiens
62 SLC30A6 55676
Proximity Label-MS Homo sapiens
63 STIM1 6786
Proximity Label-MS Homo sapiens
64 COG1 9382
Proximity Label-MS Homo sapiens
65 DMAP1 55929
Affinity Capture-MS Homo sapiens
66 KCNE3  
Affinity Capture-MS Homo sapiens
67 MAPK6  
Two-hybrid Homo sapiens
68 Uso1 56041
Affinity Capture-MS Mus musculus
69 SEC31A 22872
Affinity Capture-MS Homo sapiens
70 GALK1 2584
Affinity Capture-MS Homo sapiens
71 RHPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 TGFA  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
73 FNDC3A 22862
Proximity Label-MS Homo sapiens
74 COX2 4513
Affinity Capture-MS Homo sapiens
75 HSPD1 3329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 DOCK11 139818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 GOLGB1 2804
Proximity Label-MS Homo sapiens
78 PRKD2 25865
Two-hybrid Homo sapiens
79 SEC23IP 11196
Proximity Label-MS Homo sapiens
80 ALDH3A2 224
Proximity Label-MS Homo sapiens
81 USP46 64854
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 SCYL2 55681
Proximity Label-MS Homo sapiens
83 WWOX 51741
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 FCGRT 2217
Affinity Capture-MS Homo sapiens
85 BLZF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 HIPK3 10114
Two-hybrid Homo sapiens
87 NGEF  
Affinity Capture-MS Homo sapiens
88 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 FEM1B 10116
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 TRIP11 9321
Proximity Label-MS Homo sapiens
91 GORASP1 64689
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
92 FAM219A 203259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 GOLGA5 9950
Proximity Label-MS Homo sapiens
94 TMED3 23423
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
95 AASDH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 IGF2R 3482
Proximity Label-MS Homo sapiens
97 TMED7 51014
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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