Gene description for ALDH1A3
Gene name aldehyde dehydrogenase 1 family, member A3
Gene symbol ALDH1A3
Other names/aliases ALDH1A6
ALDH6
MCOP8
RALDH3
Species Homo sapiens
 Database cross references - ALDH1A3
ExoCarta ExoCarta_220
Entrez Gene 220
HGNC 409
MIM 600463
UniProt P47895  
 ALDH1A3 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ALDH1A3
Molecular Function
    protein homodimerization activity GO:0042803 IDA
    retinal dehydrogenase activity GO:0001758 IBA
    aldehyde dehydrogenase (NAD) activity GO:0004029 IBA
    thyroid hormone binding GO:0070324 IEA
    NAD+ binding GO:0070403 IEA
    aldehyde dehydrogenase [NAD(P)+] activity GO:0004030 IDA
Biological Process
    inner ear morphogenesis GO:0042472 IEA
    oxidation-reduction process GO:0055114 IEA
    locomotory behavior GO:0007626 IEA
    optic cup morphogenesis involved in camera-type eye development GO:0002072 IEA
    retinal metabolic process GO:0042574 IDA
    face development GO:0060324 IEA
    embryonic eye morphogenesis GO:0048048 ISS
    righting reflex GO:0060013 IEA
    positive regulation of apoptotic process GO:0043065 IEA
    retinoic acid biosynthetic process GO:0002138 IEA
    retinol metabolic process GO:0042572 IEA
    neuromuscular process controlling balance GO:0050885 IEA
    olfactory pit development GO:0060166 IEA
    retinoic acid metabolic process GO:0042573 IDA
    nucleus accumbens development GO:0021768 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ALDH1A3 in exosomes
1
Experiment ID 259
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 260
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ALDH1A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ALDH1A3 is involved
PathwayEvidenceSource
RA biosynthesis pathway TAS Reactome





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