Gene description for LSM3
Gene name LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Gene symbol LSM3
Other names/aliases SMX4
USS2
YLR438C
Species Homo sapiens
 Database cross references - LSM3
ExoCarta ExoCarta_27258
Vesiclepedia VP_27258
Entrez Gene 27258
HGNC 17874
MIM 607283
UniProt P62310  
 LSM3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
 Gene ontology annotations for LSM3
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
    U6 snRNA 3'-end binding GO:0030629 IDA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 NAS
    mRNA processing GO:0006397 TAS
    P-body assembly GO:0033962 IBA
Subcellular Localization
    P-body GO:0000932 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 TAS
    U6 snRNP GO:0005688 IBA
    cytosol GO:0005829 TAS
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IDA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IPI
    U2-type precatalytic spliceosome GO:0071005 IDA
    precatalytic spliceosome GO:0071011 IBA
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
    Lsm2-8 complex GO:0120115 IDA
    Lsm1-7-Pat1 complex GO:1990726 IBA
 Experiment description of studies that identified LSM3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for LSM3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT7 29883
Two-hybrid Homo sapiens
2 TRAPPC2 6399
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 SNRPG 6637
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 SNRPE 6635
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
6 KCTD10 83892
Affinity Capture-MS Homo sapiens
7 DPCD  
Affinity Capture-MS Homo sapiens
8 COPRS  
Affinity Capture-MS Homo sapiens
9 QKI 9444
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 GDF9 2661
Two-hybrid Homo sapiens
11 MAP4 4134
Co-fractionation Homo sapiens
12 GSTA1 2938
Two-hybrid Homo sapiens
13 LSM4 25804
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
14 PRPF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EIF4EBP2 1979
Two-hybrid Homo sapiens
16 SNRPF 6636
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
17 TRAPPC2B  
Two-hybrid Homo sapiens
18 SNRPD2 6633
Affinity Capture-MS Homo sapiens
19 LSM10  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
20 ZMAT2  
Co-fractionation Homo sapiens
21 RUVBL1 8607
Affinity Capture-MS Homo sapiens
22 CEP95  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
23 KLC2 64837
Co-fractionation Homo sapiens
24 WDR83  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 ZGPAT  
Affinity Capture-MS Homo sapiens
26 TFIP11  
Affinity Capture-MS Homo sapiens
27 XPC  
Two-hybrid Homo sapiens
28 SNRPD3 6634
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 LSM1 27257
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
30 CEP57  
Affinity Capture-MS Homo sapiens
31 SNX27 81609
Affinity Capture-MS Homo sapiens
32 PKN2 5586
Co-fractionation Homo sapiens
33 KCTD13 253980
Affinity Capture-MS Homo sapiens
34 RBM8A 9939
Co-fractionation Homo sapiens
35 LSM12 124801
Two-hybrid Homo sapiens
36 LSM2 57819
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
37 PRPF6 24148
Affinity Capture-MS Homo sapiens
38 ALDH1A3 220
Affinity Capture-MS Homo sapiens
39 HAUS2  
Affinity Capture-MS Homo sapiens
40 DLX4  
Two-hybrid Homo sapiens
41 UTP14A 10813
Two-hybrid Homo sapiens
42 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
43 MEPCE 56257
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CLNS1A 1207
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
45 SNRPD1 6632
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
46 EXOSC5 56915
Two-hybrid Homo sapiens
47 PATL1 219988
Affinity Capture-MS Homo sapiens
48 AKAP7 9465
Affinity Capture-MS Homo sapiens
49 ITGA4 3676
Affinity Capture-MS Homo sapiens
50 WHSC1L1  
Affinity Capture-MS Homo sapiens
51 CLIC4 25932
Co-fractionation Homo sapiens
52 PRPF31 26121
Affinity Capture-MS Homo sapiens
53 SF3B2 10992
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
54 LSM8 51691
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
55 NAA38  
Co-fractionation Homo sapiens
56 RC3H2  
Affinity Capture-MS Homo sapiens
57 SNRPB 6628
Affinity Capture-MS Homo sapiens
58 TCL1A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 THOC5 8563
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
60 XRN2 22803
Two-hybrid Homo sapiens
61 NACC1 112939
Two-hybrid Homo sapiens
62 SART1 9092
Affinity Capture-MS Homo sapiens
63 PIAS1 8554
Two-hybrid Homo sapiens
64 INTS3 65123
Affinity Capture-MS Homo sapiens
65 NHP2L1 4809
Affinity Capture-MS Homo sapiens
66 SART3 9733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 SH3GLB2 56904
Two-hybrid Homo sapiens
68 DDRGK1 65992
Affinity Capture-MS Homo sapiens
69 CDKN2D  
Two-hybrid Homo sapiens
70 ENTHD2  
Two-hybrid Homo sapiens
71 FARP1 10160
Affinity Capture-MS Homo sapiens
72 EEF1A1 1915
Two-hybrid Homo sapiens
73 PAXBP1  
Affinity Capture-MS Homo sapiens
74 YBX1 4904
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 LSM5 23658
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
76 ILF3 3609
Co-fractionation Homo sapiens
77 WDR77 79084
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
78 TNFAIP1 7126
Affinity Capture-MS Homo sapiens
79 KEAP1 9817
Two-hybrid Homo sapiens
80 KLF3  
Two-hybrid Homo sapiens
81 ZNF511  
Affinity Capture-MS Homo sapiens
82 HSPB1 3315
Two-hybrid Homo sapiens
83 DDIT4L  
Two-hybrid Homo sapiens
84 TUT1  
Affinity Capture-MS Homo sapiens
85 UFL1 23376
Affinity Capture-MS Homo sapiens
86 LSM7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
87 BECN1 8678
Affinity Capture-MS Homo sapiens
88 LSM6 11157
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
89 PRPF4 9128
Affinity Capture-MS Homo sapiens
90 BTBD9  
Affinity Capture-MS Homo sapiens
91 INCA1  
Two-hybrid Homo sapiens
92 PCYT1A 5130
Affinity Capture-MS Homo sapiens
93 EXOSC1 51013
Two-hybrid Homo sapiens
94 CCND3  
Two-hybrid Homo sapiens
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