Gene description for RBM8A
Gene name RNA binding motif protein 8A
Gene symbol RBM8A
Other names/aliases BOV-1A
BOV-1B
BOV-1C
C1DELq21.1
DEL1q21.1
MDS014
RBM8
RBM8B
TAR
Y14
ZNRP
ZRNP1
Species Homo sapiens
 Database cross references - RBM8A
ExoCarta ExoCarta_9939
Entrez Gene 9939
HGNC 9905
MIM 605313
UniProt Q9Y5S9  
 RBM8A identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Prostate cancer cells 25844599    
 Gene ontology annotations for RBM8A
Molecular Function
    mRNA binding GO:0003729 NAS
    RNA binding GO:0003723 NAS
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
Biological Process
    RNA splicing GO:0008380 TAS
    mRNA export from nucleus GO:0006406 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IMP
    gene expression GO:0010467 TAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    mRNA 3'-end processing GO:0031124 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    regulation of translation GO:0006417 IEA
Subcellular Localization
    nucleus GO:0005634 NAS
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IEA
    cytoplasm GO:0005737 NAS
    nucleoplasm GO:0005654 TAS
    catalytic step 2 spliceosome GO:0071013 IDA
    exon-exon junction complex GO:0035145 IDA
 Experiment description of studies that identified RBM8A in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for RBM8A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRRM1  
Invivo Homo sapiens
2 NXF1 10482
Reconstituted Complex Homo sapiens
3 MAGOHB 55110
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 MCM7 4176
Two-hybrid Homo sapiens
5 DPH2L1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
6 RNPS1 10921
Reconstituted Complex Homo sapiens
7 ALYREF 10189
Affinity Capture-Western Homo sapiens
8 IPO13  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 UPF3A  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 MAGOH  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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