Gene description for THOC1
Gene name THO complex 1
Gene symbol THOC1
Other names/aliases HPR1
P84
P84N5
Species Homo sapiens
 Database cross references - THOC1
ExoCarta ExoCarta_9984
Vesiclepedia VP_9984
Entrez Gene 9984
HGNC 19070
MIM 606930
UniProt Q96FV9  
 THOC1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for THOC1
Molecular Function
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
Biological Process
    mRNA processing GO:0006397 IEA
    mRNA export from nucleus GO:0006406 IBA
    mRNA export from nucleus GO:0006406 IMP
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 IEA
    RNA splicing GO:0008380 IEA
Subcellular Localization
    transcription export complex GO:0000346 IDA
    THO complex GO:0000347 IDA
    THO complex part of transcription export complex GO:0000445 IBA
    THO complex part of transcription export complex GO:0000445 IDA
    chromosome, telomeric region GO:0000781 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    nuclear matrix GO:0016363 IEA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified THOC1 in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for THOC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPA 6626
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 LUZP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 EXOSC7 23016
Two-hybrid Homo sapiens
5 UTRN 7402
Co-fractionation Homo sapiens
6 NCBP1 4686
Affinity Capture-MS Homo sapiens
7 DGCR14  
Two-hybrid Homo sapiens
8 RBM22 55696
Affinity Capture-MS Homo sapiens
9 ZCCHC8 55596
Co-fractionation Homo sapiens
10 USP13  
Co-fractionation Homo sapiens
11 RBM15B  
Affinity Capture-MS Homo sapiens
12 TOP3B 8940
Affinity Capture-MS Homo sapiens
13 FMR1 2332
Affinity Capture-MS Homo sapiens
14 RPA2 6118
Affinity Capture-MS Homo sapiens
15 ZC3H14  
Affinity Capture-MS Homo sapiens
16 THOC2 57187
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 FRA10AC1 118924
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 DHX8 1659
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 TDRD3  
Affinity Capture-MS Homo sapiens
20 THOC7 80145
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TNFAIP6 7130
Affinity Capture-MS Homo sapiens
22 SNRPF 6636
Affinity Capture-MS Homo sapiens
23 TRIM54  
Two-hybrid Homo sapiens
24 RNF40 9810
Affinity Capture-MS Homo sapiens
25 POLDIP3 84271
Affinity Capture-MS Homo sapiens
26 ATG4A  
Affinity Capture-MS Homo sapiens
27 SF3B1 23451
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
28 TRMT112 51504
Co-fractionation Homo sapiens
29 B4GALT7 11285
Affinity Capture-MS Homo sapiens
30 PRC1 9055
Two-hybrid Homo sapiens
31 DUSP11  
Affinity Capture-MS Homo sapiens
32 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SUPT5H 6829
Affinity Capture-MS Homo sapiens
34 Tagap  
Affinity Capture-MS Mus musculus
35 OBSL1 23363
Affinity Capture-MS Homo sapiens
36 SNRPC 6631
Affinity Capture-MS Homo sapiens
37 C15orf52 388115
Affinity Capture-MS Homo sapiens
38 SARNP 84324
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ATG16L1 55054
Affinity Capture-MS Homo sapiens
40 VPS53 55275
Co-fractionation Homo sapiens
41 CHTOP  
Affinity Capture-MS Homo sapiens
42 GTSE1 51512
Affinity Capture-MS Homo sapiens
43 MECP2 4204
Affinity Capture-MS Homo sapiens
44 MYCN  
Affinity Capture-MS Homo sapiens
45 THOC1 9984
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 NEDD4 4734
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
47 TFIP11  
Two-hybrid Homo sapiens
48 RBM8A 9939
Affinity Capture-MS Homo sapiens
49 MTNR1B  
Two-hybrid Homo sapiens
50 RB1 5925
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
51 E2F4  
Affinity Capture-MS Homo sapiens
52 PRPF6 24148
Two-hybrid Homo sapiens
53 SCAF1  
Affinity Capture-MS Homo sapiens
54 Klc4  
Two-hybrid Mus musculus
55 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
56 DDX21 9188
Affinity Capture-MS Homo sapiens
57 RBM6 10180
Affinity Capture-MS Homo sapiens
58 RRP9 9136
Co-fractionation Homo sapiens
59 C17orf85  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 C19orf47  
Affinity Capture-MS Homo sapiens
61 SP110  
Affinity Capture-MS Homo sapiens
62 MOAP1  
Two-hybrid Homo sapiens
63 ARHGAP11A  
Affinity Capture-MS Homo sapiens
64 DHX9 1660
Affinity Capture-MS Homo sapiens
65 HECTD1 25831
Affinity Capture-MS Homo sapiens
66 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
67 Ube2i  
Two-hybrid Mus musculus
68 DDX39B 7919
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ZC3H15 55854
Co-fractionation Homo sapiens
70 CD3EAP  
Proximity Label-MS Homo sapiens
71 BCLAF1 9774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 RTCB 51493
Affinity Capture-MS Homo sapiens
73 SF3B2 10992
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
74 RBM39 9584
Affinity Capture-MS Homo sapiens
75 PRPF4B 8899
Affinity Capture-MS Homo sapiens
76 OCRL 4952
Affinity Capture-MS Homo sapiens
77 SNRPB 6628
Affinity Capture-MS Homo sapiens
78 TARSL2 123283
Affinity Capture-MS Homo sapiens
79 SAP30BP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 UBR7  
Co-fractionation Homo sapiens
81 THOC5 8563
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 RBM27  
Affinity Capture-MS Homo sapiens
83 RBM33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 SF3A1 10291
Affinity Capture-MS Homo sapiens
85 XPO4 64328
Co-fractionation Homo sapiens
86 TPM1 7168
Co-fractionation Homo sapiens
87 NFKBIL1  
Affinity Capture-MS Homo sapiens
88 MYC  
Affinity Capture-MS Homo sapiens
89 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
90 CAAP1  
Affinity Capture-MS Homo sapiens
91 DLST 1743
Affinity Capture-MS Homo sapiens
92 TOP1 7150
Affinity Capture-MS Homo sapiens
93 RNF2  
Affinity Capture-MS Homo sapiens
94 USHBP1  
Two-hybrid Homo sapiens
95 FYTTD1  
Affinity Capture-MS Homo sapiens
96 MRPS17 51373
Affinity Capture-MS Homo sapiens
97 ADAR 103
Affinity Capture-MS Homo sapiens
98 ZC3H3  
Affinity Capture-MS Homo sapiens
99 ZC3H11A  
Affinity Capture-MS Homo sapiens
100 TPM3 7170
Co-fractionation Homo sapiens
101 GSN 2934
Affinity Capture-MS Homo sapiens
102 RABGEF1 27342
Two-hybrid Homo sapiens
103 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
104 EIF4A3 9775
Affinity Capture-MS Homo sapiens
105 WDR77 79084
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
106 CPSF6 11052
Affinity Capture-MS Homo sapiens
107 THOC3  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
108 DSCC1  
Co-fractionation Homo sapiens
109 FXR1 8087
Affinity Capture-MS Homo sapiens
110 RPA1 6117
Affinity Capture-MS Homo sapiens
111 MEN1 4221
Affinity Capture-MS Homo sapiens
112 ZZEF1 23140
Affinity Capture-MS Homo sapiens
113 PDHA1 5160
Affinity Capture-MS Homo sapiens
114 THRAP3 9967
Affinity Capture-MS Homo sapiens
115 THOC6 79228
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 MKI67  
Affinity Capture-MS Homo sapiens
117 DDX39A 10212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 Srsf1 110809
Affinity Capture-MS Mus musculus
119 IK 3550
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
120 ALYREF 10189
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
121 C16orf72 29035
Affinity Capture-MS Homo sapiens
122 CIT 11113
Affinity Capture-MS Homo sapiens
123 BCCIP 56647
Affinity Capture-MS Homo sapiens
124 C14orf166 51637
Affinity Capture-MS Homo sapiens
125 SNW1 22938
Two-hybrid Homo sapiens
126 KRAS 3845
Synthetic Growth Defect Homo sapiens
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
127 ZC3H13 23091
Affinity Capture-MS Homo sapiens
128 KIF1C 10749
Affinity Capture-MS Homo sapiens
129 PWWP2A  
Affinity Capture-MS Homo sapiens
130 SSRP1 6749
Affinity Capture-MS Homo sapiens
131 PRMT1 3276
Affinity Capture-MS Homo sapiens
132 ZNF830  
Co-fractionation Homo sapiens
133 PAK4 10298
Affinity Capture-MS Homo sapiens
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