Gene description for PDHA1
Gene name pyruvate dehydrogenase (lipoamide) alpha 1
Gene symbol PDHA1
Other names/aliases PDHA
PDHAD
PDHCE1A
PHE1A
Species Homo sapiens
 Database cross references - PDHA1
ExoCarta ExoCarta_5160
Entrez Gene 5160
HGNC 8806
MIM 300502
UniProt P08559  
 PDHA1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PDHA1
Molecular Function
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 IEA
    pyruvate dehydrogenase activity GO:0004738 IDA
Biological Process
    tricarboxylic acid cycle GO:0006099 IDA
    glucose metabolic process GO:0006006 IEA
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IDA
    small molecule metabolic process GO:0044281 TAS
    pyruvate metabolic process GO:0006090 TAS
    cellular metabolic process GO:0044237 TAS
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 TAS
    glycolytic process GO:0006096 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    nucleus GO:0005634 IDA
    pyruvate dehydrogenase complex GO:0045254 IDA
 Experiment description of studies that identified PDHA1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PDHA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DLAT 1737
Affinity Capture-MS Homo sapiens
2 PDHA1 5160
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 EIF6 3692
Two-hybrid Homo sapiens
4 PDHB 5162
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 DGKE  
Affinity Capture-MS Homo sapiens
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