Gene description for PDK3
Gene name pyruvate dehydrogenase kinase, isozyme 3
Gene symbol PDK3
Other names/aliases CMTX6
GS1-358P8.4
Species Homo sapiens
 Database cross references - PDK3
ExoCarta ExoCarta_5165
Vesiclepedia VP_5165
Entrez Gene 5165
HGNC 8811
MIM 300906
UniProt Q15120  
 PDK3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PDK3
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IBA
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
Biological Process
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 IBA
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 IMP
    regulation of glucose metabolic process GO:0010906 IBA
    regulation of glucose metabolic process GO:0010906 IMP
    peptidyl-serine phosphorylation GO:0018105 IDA
    peroxisome proliferator activated receptor signaling pathway GO:0035357 IMP
    cellular response to fatty acid GO:0071398 IMP
    hypoxia-inducible factor-1alpha signaling pathway GO:0097411 IMP
    regulation of reactive oxygen species metabolic process GO:2000377 IMP
Subcellular Localization
    nucleolus GO:0005730 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified PDK3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HLTF  
Affinity Capture-MS Homo sapiens
2 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
3 UTRN 7402
Affinity Capture-MS Homo sapiens
4 CCT3 7203
Affinity Capture-MS Homo sapiens
5 STAT5A 6776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RPA2 6118
Proximity Label-MS Homo sapiens
7 TRUB2  
Proximity Label-MS Homo sapiens
8 MTG2  
Proximity Label-MS Homo sapiens
9 FPR1  
Affinity Capture-MS Homo sapiens
10 CCT4 10575
Affinity Capture-MS Homo sapiens
11 RPUSD4 84881
Proximity Label-MS Homo sapiens
12 MTRF1  
Proximity Label-MS Homo sapiens
13 APP 351
Reconstituted Complex Homo sapiens
14 CCDC90B  
Proximity Label-MS Homo sapiens
15 LRPPRC 10128
Proximity Label-MS Homo sapiens
16 TFAM 7019
Proximity Label-MS Homo sapiens
17 PMPCA 23203
Proximity Label-MS Homo sapiens
18 ACAP2 23527
Affinity Capture-MS Homo sapiens
19 HINT2 84681
Proximity Label-MS Homo sapiens
20 PDK2 5164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 EXD2  
Proximity Label-MS Homo sapiens
22 TULP3 7289
Affinity Capture-MS Homo sapiens
23 FOXD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SSBP1 6742
Proximity Label-MS Homo sapiens
25 PMPCB 9512
Proximity Label-MS Homo sapiens
26 TACO1  
Proximity Label-MS Homo sapiens
27 FASTKD2  
Proximity Label-MS Homo sapiens
28 VWA8 23078
Proximity Label-MS Homo sapiens
29 PDHA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MDH2 4191
Proximity Label-MS Homo sapiens
31 PDK1 5163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DLD 1738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TCP1 6950
Affinity Capture-MS Homo sapiens
34 DNLZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 C6orf203  
Proximity Label-MS Homo sapiens
36 FASTKD5  
Proximity Label-MS Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 CCT5 22948
Affinity Capture-MS Homo sapiens
39 TUFM 7284
Proximity Label-MS Homo sapiens
40 HSCB 150274
Proximity Label-MS Homo sapiens
41 VCP 7415
Affinity Capture-MS Homo sapiens
42 PDHX 8050
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TBRG4 9238
Proximity Label-MS Homo sapiens
44 HECTD1 25831
Affinity Capture-MS Homo sapiens
45 MRPL11 65003
Proximity Label-MS Homo sapiens
46 MTRF1L  
Proximity Label-MS Homo sapiens
47 MRRF  
Proximity Label-MS Homo sapiens
48 MTIF2 4528
Proximity Label-MS Homo sapiens
49 ECD  
Affinity Capture-MS Homo sapiens
50 MTIF3  
Proximity Label-MS Homo sapiens
51 CCT6A 908
Affinity Capture-MS Homo sapiens
52 CUL3 8452
Affinity Capture-MS Homo sapiens
53 UBR7  
Affinity Capture-MS Homo sapiens
54 RMND1 55005
Proximity Label-MS Homo sapiens
55 EGFR 1956
PCA Homo sapiens
56 DHX30 22907
Proximity Label-MS Homo sapiens
57 CECR5 27440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RPA3 6119
Proximity Label-MS Homo sapiens
59 CCT8 10694
Affinity Capture-MS Homo sapiens
60 CCT2 10576
Affinity Capture-MS Homo sapiens
61 ACAD9 28976
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 HSPD1 3329
Affinity Capture-MS Homo sapiens
63 DLAT 1737
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 METTL15  
Proximity Label-MS Homo sapiens
65 GFM2 84340
Proximity Label-MS Homo sapiens
66 CLPP 8192
Proximity Label-MS Homo sapiens
67 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
68 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
69 TSFM 10102
Proximity Label-MS Homo sapiens
70 AUH 549
Proximity Label-MS Homo sapiens
71 ICT1 3396
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
72 EIF1AX 1964
Affinity Capture-MS Homo sapiens
73 LONP1 9361
Proximity Label-MS Homo sapiens
74 PDHA1 5160
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 PDHB 5162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 COX8A  
Proximity Label-MS Homo sapiens
78 C21orf33  
Proximity Label-MS Homo sapiens
79 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
80 CCT7 10574
Affinity Capture-MS Homo sapiens
81 MIF 4282
Affinity Capture-MS Homo sapiens
82 GFM1 85476
Proximity Label-MS Homo sapiens
83 AARS2  
Proximity Label-MS Homo sapiens
84 HSF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
85 PDK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 C1QBP 708
Proximity Label-MS Homo sapiens
87 METTL17  
Proximity Label-MS Homo sapiens
88 YBEY  
Affinity Capture-MS Homo sapiens
89 SYT12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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