Gene description for RPUSD4
Gene name RNA pseudouridylate synthase domain containing 4
Gene symbol RPUSD4
Other names/aliases -
Species Homo sapiens
 Database cross references - RPUSD4
ExoCarta ExoCarta_84881
Entrez Gene 84881
HGNC 25898
UniProt Q96CM3  
 RPUSD4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for RPUSD4
Molecular Function
    pseudouridine synthase activity GO:0009982 IEA
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
Biological Process
    pseudouridine synthesis GO:0001522 IEA
Subcellular Localization
    mitochondrion GO:0005739 IEA
 Experiment description of studies that identified RPUSD4 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for RPUSD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RPUSD4 is involved
No pathways found





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