Gene description for RPUSD4
Gene name RNA pseudouridylate synthase domain containing 4
Gene symbol RPUSD4
Other names/aliases -
Species Homo sapiens
 Database cross references - RPUSD4
ExoCarta ExoCarta_84881
Vesiclepedia VP_84881
Entrez Gene 84881
HGNC 25898
UniProt Q96CM3  
 RPUSD4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for RPUSD4
Molecular Function
    tRNA binding GO:0000049 IDA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    pseudouridine synthase activity GO:0009982 IDA
    pseudouridine synthase activity GO:0009982 IMP
    tRNA pseudouridine synthase activity GO:0106029 IEA
    mitochondrial ribosomal large subunit rRNA binding GO:1990400 IDA
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    rRNA pseudouridine synthesis GO:0031118 IMP
    positive regulation of mitochondrial translation GO:0070131 IMP
    mitochondrial tRNA pseudouridine synthesis GO:0070902 IMP
    mRNA pseudouridine synthesis GO:1990481 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IDA
    ribonucleoprotein granule GO:0035770 IDA
 Experiment description of studies that identified RPUSD4 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for RPUSD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NUPL2 11097
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 AFG3L2 10939
Proximity Label-MS Homo sapiens
4 HNRNPC 3183
Affinity Capture-MS Homo sapiens
5 Tmed2 56334
Affinity Capture-MS Mus musculus
6 MRPL48  
Proximity Label-MS Homo sapiens
7 Cul1 26965
Affinity Capture-MS Mus musculus
8 THEM4  
Proximity Label-MS Homo sapiens
9 ATP5J2-PTCD1  
Proximity Label-MS Homo sapiens
10 CCL3  
Affinity Capture-MS Homo sapiens
11 SLIRP 81892
Proximity Label-MS Homo sapiens
12 NDUFS1 4719
Proximity Label-MS Homo sapiens
13 MRPS30 10884
Proximity Label-MS Homo sapiens
14 TRMT61B  
Proximity Label-MS Homo sapiens
15 MRPL17  
Proximity Label-MS Homo sapiens
16 ATE1 11101
Affinity Capture-MS Homo sapiens
17 ANGEL2 90806
Proximity Label-MS Homo sapiens
18 GTPBP10  
Proximity Label-MS Homo sapiens
19 PYCR1 5831
Proximity Label-MS Homo sapiens
20 PDK3 5165
Proximity Label-MS Homo sapiens
21 TRUB2  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Chmp4b 75608
Affinity Capture-MS Mus musculus
23 NGRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MRPL24  
Proximity Label-MS Homo sapiens
25 MRPL37 51253
Proximity Label-MS Homo sapiens
26 ETFB 2109
Proximity Label-MS Homo sapiens
27 NDUFS7 374291
Proximity Label-MS Homo sapiens
28 MRPL50 54534
Proximity Label-MS Homo sapiens
29 MRPL23 6150
Proximity Label-MS Homo sapiens
30 PDHB 5162
Proximity Label-MS Homo sapiens
31 MRPL49 740
Proximity Label-MS Homo sapiens
32 MRPS27 23107
Proximity Label-MS Homo sapiens
33 PDE12 201626
Proximity Label-MS Homo sapiens
34 NDUFS2 4720
Proximity Label-MS Homo sapiens
35 SUPV3L1 6832
Proximity Label-MS Homo sapiens
36 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
37 LRPPRC 10128
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
38 RNH1 6050
Affinity Capture-MS Homo sapiens
39 KBTBD6  
Affinity Capture-MS Homo sapiens
40 XRCC1 7515
Affinity Capture-MS Homo sapiens
41 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
42 MRPL20 55052
Proximity Label-MS Homo sapiens
43 NDUFS3 4722
Proximity Label-MS Homo sapiens
44 OGDH 4967
Proximity Label-MS Homo sapiens
45 PRDX3 10935
Proximity Label-MS Homo sapiens
46 IARS2 55699
Proximity Label-MS Homo sapiens
47 FHL3 2275
Proximity Label-MS Homo sapiens
48 NDUFA7 4701
Proximity Label-MS Homo sapiens
49 HARS2 23438
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MRPS5 64969
Proximity Label-MS Homo sapiens
51 YARS2  
Affinity Capture-MS Homo sapiens
52 HINT2 84681
Proximity Label-MS Homo sapiens
53 MRPS23 51649
Proximity Label-MS Homo sapiens
54 MRPS24 64951
Proximity Label-MS Homo sapiens
55 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
56 FBXW8 26259
Affinity Capture-MS Homo sapiens
57 MRPL21 219927
Proximity Label-MS Homo sapiens
58 OBSL1 23363
Affinity Capture-MS Homo sapiens
59 MRPS26 64949
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
60 MALSU1  
Affinity Capture-MS Homo sapiens
61 ACOT2 10965
Proximity Label-MS Homo sapiens
62 PTCD1 26024
Affinity Capture-MS Homo sapiens
63 NNT 23530
Proximity Label-MS Homo sapiens
64 HINFP  
Two-hybrid Homo sapiens
65 ERAL1  
Proximity Label-MS Homo sapiens
66 Ubr5  
Affinity Capture-MS Mus musculus
67 MRPL39 54148
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
68 POLRMT 5442
Proximity Label-MS Homo sapiens
69 NDUFV1 4723
Proximity Label-MS Homo sapiens
70 ATG16L1 55054
Affinity Capture-MS Homo sapiens
71 ETFA 2108
Proximity Label-MS Homo sapiens
72 PHB 5245
Proximity Label-MS Homo sapiens
73 ATP5H 10476
Proximity Label-MS Homo sapiens
74 POTEE 445582
Proximity Label-MS Homo sapiens
75 BCS1L 617
Proximity Label-MS Homo sapiens
76 MYCN  
Affinity Capture-MS Homo sapiens
77 MRPL4 51073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
78 FASTKD2  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 NME4 4833
Proximity Label-MS Homo sapiens
80 NDUFA6  
Proximity Label-MS Homo sapiens
81 MTIF2 4528
Proximity Label-MS Homo sapiens
82 MRPL47 57129
Proximity Label-MS Homo sapiens
83 FAM120A 23196
Affinity Capture-MS Homo sapiens
84 MTERF3  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 PEX7  
Affinity Capture-MS Homo sapiens
86 Tmed10 68581
Affinity Capture-MS Mus musculus
87 NOTCH2 4853
Affinity Capture-MS Homo sapiens
88 MDH2 4191
Proximity Label-MS Homo sapiens
89 STOML2 30968
Proximity Label-MS Homo sapiens
90 MRPL38  
Proximity Label-MS Homo sapiens
91 ALAS1  
Proximity Label-MS Homo sapiens
92 CAMK2A 815
Affinity Capture-MS Homo sapiens
93 EIF2AK2 5610
Proximity Label-MS Homo sapiens
94 MRPS25 64432
Proximity Label-MS Homo sapiens
95 MRPS33  
Proximity Label-MS Homo sapiens
96 C6orf203  
Proximity Label-MS Homo sapiens
97 NDUFS4 4724
Proximity Label-MS Homo sapiens
98 IKBIP 121457
Co-fractionation Homo sapiens
99 FASTKD5  
Proximity Label-MS Homo sapiens
100 MRPL45 84311
Proximity Label-MS Homo sapiens
101 MRPS34 65993
Proximity Label-MS Homo sapiens
102 CENPV 201161
Proximity Label-MS Homo sapiens
103 SRSF3 6428
Affinity Capture-MS Homo sapiens
104 YBX2 51087
Affinity Capture-MS Homo sapiens
105 ATPAF1  
Proximity Label-MS Homo sapiens
106 PAM16  
Proximity Label-MS Homo sapiens
107 TMED4 222068
Affinity Capture-MS Homo sapiens
108 PTCD3 55037
Proximity Label-MS Homo sapiens
109 NDUFS8 4728
Proximity Label-MS Homo sapiens
110 MMAB 326625
Proximity Label-MS Homo sapiens
111 MRPL51 51258
Affinity Capture-MS Homo sapiens
112 MRPS6  
Proximity Label-MS Homo sapiens
113 SYNCRIP 10492
Co-fractionation Homo sapiens
114 PNMA2  
Affinity Capture-MS Homo sapiens
115 NDUFA2 4695
Proximity Label-MS Homo sapiens
116 C10orf2 56652
Proximity Label-MS Homo sapiens
117 CYC1 1537
Co-fractionation Homo sapiens
118 MRPL3  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 DBT 1629
Proximity Label-MS Homo sapiens
120 AARS2  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
121 MRPL11 65003
Proximity Label-MS Homo sapiens
122 NIPSNAP1 8508
Proximity Label-MS Homo sapiens
123 ATP5B 506
Proximity Label-MS Homo sapiens
124 PIPSL 266971
Affinity Capture-MS Homo sapiens
125 MRPL41 64975
Proximity Label-MS Homo sapiens
126 HNRNPDL 9987
Proximity Label-MS Homo sapiens
127 IMMT 10989
Proximity Label-MS Homo sapiens
128 MRPL44  
Proximity Label-MS Homo sapiens
129 ACAT1 38
Proximity Label-MS Homo sapiens
130 MRPL40 64976
Proximity Label-MS Homo sapiens
131 WBSCR16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 MAP7 9053
Affinity Capture-MS Homo sapiens
133 NDUFV3 4731
Proximity Label-MS Homo sapiens
134 MRPS12  
Proximity Label-MS Homo sapiens
135 DAP3 7818
Proximity Label-MS Homo sapiens
136 TRMT10C 54931
Proximity Label-MS Homo sapiens
137 PNPT1 87178
Proximity Label-MS Homo sapiens
138 Cct3 12462
Affinity Capture-MS Mus musculus
139 NDUFA9 4704
Proximity Label-MS Homo sapiens
140 NDUFV2 4729
Proximity Label-MS Homo sapiens
141 LETM1 3954
Proximity Label-MS Homo sapiens
142 MRPS7 51081
Proximity Label-MS Homo sapiens
143 MRPL9 65005
Proximity Label-MS Homo sapiens
144 MRPS14  
Proximity Label-MS Homo sapiens
145 NFS1 9054
Proximity Label-MS Homo sapiens
146 ATP5F1 515
Proximity Label-MS Homo sapiens
147 MRPL42  
Proximity Label-MS Homo sapiens
148 EGFR 1956
Negative Genetic Homo sapiens
149 HSD17B10 3028
Proximity Label-MS Homo sapiens
150 ELAC2 60528
Proximity Label-MS Homo sapiens
151 GADD45GIP1  
Proximity Label-MS Homo sapiens
152 DHX30 22907
Proximity Label-MS Homo sapiens
153 VARS2  
Proximity Label-MS Homo sapiens
154 PYCR2 29920
Proximity Label-MS Homo sapiens
155 KBTBD7  
Affinity Capture-MS Homo sapiens
156 ECHS1 1892
Proximity Label-MS Homo sapiens
157 NXF1 10482
Affinity Capture-RNA Homo sapiens
158 MRPL15 29088
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 KLHL22 84861
Affinity Capture-MS Homo sapiens
160 C12orf65  
Proximity Label-MS Homo sapiens
161 PRR3  
Affinity Capture-MS Homo sapiens
162 UBR1 197131
Affinity Capture-MS Homo sapiens
163 RBM42  
Affinity Capture-MS Homo sapiens
164 MYC  
Affinity Capture-MS Homo sapiens
165 TACO1  
Proximity Label-MS Homo sapiens
166 DLST 1743
Proximity Label-MS Homo sapiens
167 PREPL 9581
Proximity Label-MS Homo sapiens
168 HSDL2 84263
Proximity Label-MS Homo sapiens
169 CLPX 10845
Proximity Label-MS Homo sapiens
170 ACAD9 28976
Proximity Label-MS Homo sapiens
171 MRPS36 92259
Proximity Label-MS Homo sapiens
172 VWA8 23078
Proximity Label-MS Homo sapiens
173 HSPD1 3329
Proximity Label-MS Homo sapiens
174 HSPA9 3313
Proximity Label-MS Homo sapiens
175 DLAT 1737
Proximity Label-MS Homo sapiens
176 METTL15  
Proximity Label-MS Homo sapiens
177 CAMK2G 818
Affinity Capture-MS Homo sapiens
178 RBMS2 5939
Affinity Capture-MS Homo sapiens
179 RBM11  
Proximity Label-MS Homo sapiens
180 PPIF 10105
Proximity Label-MS Homo sapiens
181 C17orf70  
Affinity Capture-MS Homo sapiens
182 ZRANB1 54764
Affinity Capture-MS Homo sapiens
183 NDUFAF2  
Proximity Label-MS Homo sapiens
184 FTL 2512
Affinity Capture-MS Homo sapiens
185 VWCE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
186 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
187 GLS 2744
Proximity Label-MS Homo sapiens
188 ATP5J 522
Proximity Label-MS Homo sapiens
189 TSFM 10102
Proximity Label-MS Homo sapiens
190 RBM45  
Affinity Capture-MS Homo sapiens
191 MRPS2 51116
Proximity Label-MS Homo sapiens
192 MRPS9 64965
Proximity Label-MS Homo sapiens
193 MRPL22  
Proximity Label-MS Homo sapiens
194 MCAT 27349
Affinity Capture-MS Homo sapiens
195 TP53 7157
Synthetic Growth Defect Homo sapiens
Negative Genetic Homo sapiens
196 ICT1 3396
Proximity Label-MS Homo sapiens
197 MRPS28  
Proximity Label-MS Homo sapiens
198 LARS2 23395
Proximity Label-MS Homo sapiens
199 ABHD10 55347
Proximity Label-MS Homo sapiens
200 MRPS18C  
Proximity Label-MS Homo sapiens
201 MRPL53  
Proximity Label-MS Homo sapiens
202 MRPL43 84545
Proximity Label-MS Homo sapiens
203 PDHA1 5160
Proximity Label-MS Homo sapiens
204 MRPS17 51373
Proximity Label-MS Homo sapiens
205 DNAJA3 9093
Proximity Label-MS Homo sapiens
206 MRPS35 60488
Proximity Label-MS Homo sapiens
207 MRPL1  
Proximity Label-MS Homo sapiens
208 NDUFA12 55967
Proximity Label-MS Homo sapiens
209 GRPEL1 80273
Proximity Label-MS Homo sapiens
210 COX8A  
Proximity Label-MS Homo sapiens
211 MRPS10 55173
Proximity Label-MS Homo sapiens
212 NUDT19 390916
Proximity Label-MS Homo sapiens
213 SHMT2 6472
Proximity Label-MS Homo sapiens
214 KCNE3  
Affinity Capture-MS Homo sapiens
215 NDUFS6  
Proximity Label-MS Homo sapiens
216 GUF1  
Proximity Label-MS Homo sapiens
217 NDUFA5 4698
Proximity Label-MS Homo sapiens
218 MRPS18B 28973
Proximity Label-MS Homo sapiens
219 ATP5D 513
Proximity Label-MS Homo sapiens
220 MOV10 4343
Affinity Capture-RNA Homo sapiens
Affinity Capture-MS Homo sapiens
221 RNMTL1  
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
222 MRPL13  
Proximity Label-MS Homo sapiens
223 ACOT1 641371
Proximity Label-MS Homo sapiens
224 MRPL46  
Proximity Label-MS Homo sapiens
225 MGME1  
Proximity Label-MS Homo sapiens
226 MRPL19 9801
Proximity Label-MS Homo sapiens
227 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
228 RPUSD3  
Affinity Capture-MS Homo sapiens
229 MRPS16  
Proximity Label-MS Homo sapiens
230 GRSF1 2926
Proximity Label-MS Homo sapiens
231 TMED7 51014
Affinity Capture-MS Homo sapiens
232 NDUFAF4 29078
Proximity Label-MS Homo sapiens
233 QRSL1  
Proximity Label-MS Homo sapiens
234 HNRNPU 3192
Affinity Capture-MS Homo sapiens
235 MRPL2 51069
Proximity Label-MS Homo sapiens
236 MRPL55  
Proximity Label-MS Homo sapiens
237 MRPS31  
Proximity Label-MS Homo sapiens
238 C17orf80 55028
Proximity Label-MS Homo sapiens
239 METTL17  
Proximity Label-MS Homo sapiens
240 MRPS22 56945
Proximity Label-MS Homo sapiens
241 HNRNPL 3191
Proximity Label-MS Homo sapiens
242 SUCLA2 8803
Proximity Label-MS Homo sapiens
243 MRPL28 10573
Proximity Label-MS Homo sapiens
244 KCTD5 54442
Affinity Capture-MS Homo sapiens
245 MRPS18A  
Proximity Label-MS Homo sapiens
246 DDX28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
247 TIMM44 10469
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RPUSD4 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here