Gene description for MCAT
Gene name malonyl CoA:ACP acyltransferase (mitochondrial)
Gene symbol MCAT
Other names/aliases FASN2C
MCT
MT
NET62
fabD
Species Homo sapiens
 Database cross references - MCAT
ExoCarta ExoCarta_27349
Vesiclepedia VP_27349
Entrez Gene 27349
HGNC 29622
MIM 614479
UniProt Q8IVS2  
 MCAT identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MCAT
Molecular Function
    RNA binding GO:0003723 HDA
    [acyl-carrier-protein] S-malonyltransferase activity GO:0004314 IBA
    [acyl-carrier-protein] S-malonyltransferase activity GO:0004314 IDA
    [acyl-carrier-protein] S-malonyltransferase activity GO:0004314 TAS
Biological Process
    fatty acid biosynthetic process GO:0006633 IBA
    fatty acid biosynthetic process GO:0006633 IDA
    fatty acid biosynthetic process GO:0006633 IEA
    fatty acid beta-oxidation GO:0006635 TAS
    mitochondrial small ribosomal subunit assembly GO:0180026 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified MCAT in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MCAT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 RBMS3  
Affinity Capture-MS Homo sapiens
3 MRPL30  
Affinity Capture-MS Homo sapiens
4 ZNF324B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MRPS27 23107
Affinity Capture-MS Homo sapiens
6 TRUB2  
Affinity Capture-MS Homo sapiens
7 NGRN  
Affinity Capture-MS Homo sapiens
8 RPL10 6134
Affinity Capture-MS Homo sapiens
9 NOA1  
Affinity Capture-MS Homo sapiens
10 RPUSD4 84881
Affinity Capture-MS Homo sapiens
11 PTPN1 5770
Co-fractionation Homo sapiens
12 ANKRD28 23243
Affinity Capture-MS Homo sapiens
13 TAF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MRPS18C  
Affinity Capture-MS Homo sapiens
15 RBM3 5935
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MRPS5 64969
Affinity Capture-MS Homo sapiens
17 YARS2  
Affinity Capture-MS Homo sapiens
18 MRPS23 51649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MRPS24 64951
Affinity Capture-MS Homo sapiens
20 RXRB 6257
Affinity Capture-MS Homo sapiens
21 EWSR1 2130
Co-fractionation Homo sapiens
22 TFEB  
Affinity Capture-MS Homo sapiens
23 MRPS26 64949
Affinity Capture-MS Homo sapiens
24 MALSU1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 Ncstn 59287
Affinity Capture-MS Mus musculus
26 PTCD1 26024
Affinity Capture-MS Homo sapiens
27 ERAL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 Ubr5  
Affinity Capture-MS Mus musculus
29 MRPL39 54148
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MRPS7 51081
Affinity Capture-MS Homo sapiens
31 PPP6R3 55291
Affinity Capture-MS Homo sapiens
32 POLR2M  
Affinity Capture-MS Homo sapiens
33 MYCN  
Affinity Capture-MS Homo sapiens
34 MRPL4 51073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 FASTKD2  
Affinity Capture-MS Homo sapiens
36 PREPL 9581
Affinity Capture-MS Homo sapiens
37 MMGT1 93380
Affinity Capture-MS Homo sapiens
38 FAM120A 23196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MTERF3  
Affinity Capture-MS Homo sapiens
40 RBMX 27316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ALAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 MRPS25 64432
Affinity Capture-MS Homo sapiens
43 ZNF133  
Affinity Capture-MS Homo sapiens
44 TAOK1 57551
Affinity Capture-MS Homo sapiens
45 MRPS34 65993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SHMT1 6470
Co-fractionation Homo sapiens
47 TMEM43 79188
Co-fractionation Homo sapiens
48 SRSF3 6428
Affinity Capture-MS Homo sapiens
49 PTCD3 55037
Affinity Capture-MS Homo sapiens
50 Ten1  
Affinity Capture-MS Mus musculus
51 MRPL51 51258
Affinity Capture-MS Homo sapiens
52 MRPS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 DBT 1629
Affinity Capture-MS Homo sapiens
54 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
55 ZYG11B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 MRPS33  
Affinity Capture-MS Homo sapiens
57 REEP5 7905
Affinity Capture-MS Homo sapiens
58 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 CIRBP 1153
Affinity Capture-MS Homo sapiens
60 WBSCR16  
Affinity Capture-MS Homo sapiens
61 DAP3 7818
Affinity Capture-MS Homo sapiens
62 MRPS35 60488
Affinity Capture-MS Homo sapiens
63 Wdr5  
Affinity Capture-MS Mus musculus
64 MRPS11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 C1orf52  
Affinity Capture-MS Homo sapiens
66 EGFR 1956
Negative Genetic Homo sapiens
67 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 MRPS28  
Affinity Capture-MS Homo sapiens
69 PRR3  
Affinity Capture-MS Homo sapiens
70 MYC  
Affinity Capture-MS Homo sapiens
71 RPA3 6119
Proximity Label-MS Homo sapiens
72 Chmp3  
Affinity Capture-MS Mus musculus
73 MRPS17 51373
Affinity Capture-MS Homo sapiens
74 RBMS2 5939
Affinity Capture-MS Homo sapiens
75 ZNF574  
Affinity Capture-MS Homo sapiens
76 EIF1AD 84285
Affinity Capture-MS Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 SPTAN1 6709
Co-fractionation Homo sapiens
79 MRPS9 64965
Affinity Capture-MS Homo sapiens
80 MRPS2 51116
Affinity Capture-MS Homo sapiens
81 DOCK5 80005
Affinity Capture-MS Homo sapiens
82 ICT1 3396
Affinity Capture-MS Homo sapiens
83 MRPS31  
Affinity Capture-MS Homo sapiens
84 AGMAT  
Co-fractionation Homo sapiens
85 MRPS15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 MRPS10 55173
Affinity Capture-MS Homo sapiens
87 RPUSD2  
Co-fractionation Homo sapiens
88 MRPS21  
Affinity Capture-MS Homo sapiens
89 RAVER1 125950
Co-fractionation Homo sapiens
90 ITGAV 3685
Co-fractionation Homo sapiens
91 MRPS18B 28973
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 ALYREF 10189
Affinity Capture-MS Homo sapiens
93 DDX28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 RPUSD3  
Affinity Capture-MS Homo sapiens
95 MRPS16  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
96 HNRNPU 3192
Affinity Capture-MS Homo sapiens
97 TAP1 6890
Co-fractionation Homo sapiens
98 SLC33A1 9197
Affinity Capture-MS Homo sapiens
99 USF1  
Affinity Capture-MS Homo sapiens
100 METTL17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 MRPS22 56945
Affinity Capture-MS Homo sapiens
102 SPCS2 9789
Co-fractionation Homo sapiens
103 EP300 2033
Affinity Capture-MS Homo sapiens
104 TAOK2 9344
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MCAT is involved
PathwayEvidenceSource
Fatty acid metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Mitochondrial Fatty Acid Beta-Oxidation TAS Reactome





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