Gene description for ANKRD28
Gene name ankyrin repeat domain 28
Gene symbol ANKRD28
Other names/aliases PITK
PPP1R65
Species Homo sapiens
 Database cross references - ANKRD28
ExoCarta ExoCarta_23243
Vesiclepedia VP_23243
Entrez Gene 23243
HGNC 29024
MIM 611122
UniProt O15084  
 ANKRD28 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ANKRD28
Molecular Function
    protein binding GO:0005515 IPI
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ANKRD28 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ANKRD28
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CDC14B 8555
Affinity Capture-MS Homo sapiens
3 SMC3 9126
Proximity Label-MS Homo sapiens
4 AP2B1 163
Proximity Label-MS Homo sapiens
5 HOOK1  
Proximity Label-MS Homo sapiens
6 RASSF8 11228
Proximity Label-MS Homo sapiens
7 PLA2G4A 5321
Co-fractionation Homo sapiens
8 HOXA7  
Affinity Capture-MS Homo sapiens
9 RAMP1  
Affinity Capture-MS Homo sapiens
10 REL 5966
Co-fractionation Homo sapiens
11 TRIM66  
Affinity Capture-MS Homo sapiens
12 APC  
Proximity Label-MS Homo sapiens
13 HOXC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 BRCA1 672
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 RPL10 6134
Affinity Capture-MS Homo sapiens
16 TRIM33 51592
Affinity Capture-MS Homo sapiens
17 EPS8 2059
Proximity Label-MS Homo sapiens
18 PPP1R9B 84687
Proximity Label-MS Homo sapiens
19 CAMSAP2  
Proximity Label-MS Homo sapiens
20 PRKDC 5591
Affinity Capture-MS Homo sapiens
21 AKAP8L  
Affinity Capture-MS Homo sapiens
22 PTRH1  
Two-hybrid Homo sapiens
23 KIAA1429 25962
Affinity Capture-MS Homo sapiens
24 TTLL4  
Affinity Capture-MS Homo sapiens
25 SHKBP1  
Proximity Label-MS Homo sapiens
26 RUVBL1 8607
Co-fractionation Homo sapiens
27 ARHGEF2 9181
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 AP2A2 161
Affinity Capture-MS Homo sapiens
29 RAB40C 57799
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 SDCCAG3  
Proximity Label-MS Homo sapiens
31 ZNF512B  
Two-hybrid Homo sapiens
32 PPP6R1 22870
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
33 KRI1  
Proximity Label-MS Homo sapiens
34 AGPAT2  
Two-hybrid Homo sapiens
35 DNAJB1 3337
Proximity Label-MS Homo sapiens
36 FOXD4L6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CEP162  
Proximity Label-MS Homo sapiens
38 ICAM4  
Affinity Capture-MS Homo sapiens
39 ABT1 29777
Affinity Capture-MS Homo sapiens
40 MRPS7 51081
Affinity Capture-MS Homo sapiens
41 PPP6R3 55291
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 GPS1 2873
Co-fractionation Homo sapiens
43 SDCBP 6386
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CTTNBP2NL  
Co-fractionation Homo sapiens
45 CSNK2B 1460
Two-hybrid Homo sapiens
46 CUL1 8454
Co-fractionation Homo sapiens
47 AMOT  
Proximity Label-MS Homo sapiens
48 CTCF  
Co-fractionation Homo sapiens
49 NOTCH2 4853
Proximity Label-MS Homo sapiens
50 PFN1 5216
Proximity Label-MS Homo sapiens
51 BAG5 9529
Affinity Capture-MS Homo sapiens
52 KRT8 3856
Proximity Label-MS Homo sapiens
53 TRIM41 90933
Proximity Label-MS Homo sapiens
54 USP10 9100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
55 USP21  
Affinity Capture-MS Homo sapiens
56 CD74 972
Affinity Capture-MS Homo sapiens
57 TGM2 7052
Two-hybrid Homo sapiens
58 TBKBP1  
Proximity Label-MS Homo sapiens
59 PPP6R2 9701
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 PLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CEP192 55125
Proximity Label-MS Homo sapiens
62 Ppp6c 67857
Affinity Capture-MS Mus musculus
63 DNAJC2 27000
Proximity Label-MS Homo sapiens
64 PPIP5K2 23262
Affinity Capture-MS Homo sapiens
65 FAM193A  
Proximity Label-MS Homo sapiens
66 COMTD1 118881
Affinity Capture-MS Homo sapiens
67 NECAP2 55707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 UBR5 51366
Proximity Label-MS Homo sapiens
69 CIRH1A 84916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 PDGFRL 5157
Affinity Capture-MS Homo sapiens
71 NTRK1 4914
Affinity Capture-MS Homo sapiens
72 FOXK2  
Affinity Capture-MS Homo sapiens
73 NEK4  
Proximity Label-MS Homo sapiens
74 GPR182  
Affinity Capture-MS Homo sapiens
75 PLK1 5347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 DCTN1 1639
Proximity Label-MS Homo sapiens
77 Arhgef2  
Affinity Capture-MS Mus musculus
78 SMC1A 8243
Proximity Label-MS Homo sapiens
79 FNBP1 23048
Co-fractionation Homo sapiens
80 HECTD1 25831
Proximity Label-MS Homo sapiens
81 TRMT2A 27037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 NFKB2 4791
Co-fractionation Homo sapiens
83 AGPS 8540
Proximity Label-MS Homo sapiens
84 ANKRD44 91526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 RAD21 5885
Proximity Label-MS Homo sapiens
86 NFATC4 4776
Proximity Label-MS Homo sapiens
87 LRCH3 84859
Proximity Label-MS Homo sapiens
88 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
89 EIF3G 8666
Co-fractionation Homo sapiens
90 IQSEC1 9922
Affinity Capture-MS Homo sapiens
91 MOB1B 92597
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
92 LEO1 123169
Co-fractionation Homo sapiens
93 TMIE  
Two-hybrid Homo sapiens
94 FGF14  
Affinity Capture-MS Homo sapiens
95 C9orf78 51759
Affinity Capture-MS Homo sapiens
96 SIRT7  
Affinity Capture-MS Homo sapiens
97 CDKL1  
Affinity Capture-MS Homo sapiens
98 SNX18 112574
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
99 CUL3 8452
Affinity Capture-MS Homo sapiens
100 DCLRE1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 GADD45GIP1  
Two-hybrid Homo sapiens
102 FBXO6 26270
Affinity Capture-MS Homo sapiens
103 PRPSAP1 5635
Affinity Capture-MS Homo sapiens
104 SNX27 81609
Affinity Capture-MS Homo sapiens
105 EIF3A 8661
Co-fractionation Homo sapiens
106 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 HOOK3 84376
Proximity Label-MS Homo sapiens
108 FLNC 2318
Co-fractionation Homo sapiens
109 ANKRD52 283373
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 CAMK4 814
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 ARHGAP29 9411
Co-fractionation Homo sapiens
112 MYO6 4646
Proximity Label-MS Homo sapiens
113 IRF5  
Proximity Label-MS Homo sapiens
114 GPR45  
Affinity Capture-MS Homo sapiens
115 SEC16A 9919
Proximity Label-MS Homo sapiens
116 MGC50722  
Two-hybrid Homo sapiens
117 GOLGA1  
Proximity Label-MS Homo sapiens
118 HSPA1A 3303
Affinity Capture-MS Homo sapiens
119 PPP6C 5537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 RCN2 5955
Proximity Label-MS Homo sapiens
121 DDRGK1 65992
Affinity Capture-MS Homo sapiens
122 CTNNA1 1495
Proximity Label-MS Homo sapiens
123 FBF1  
Proximity Label-MS Homo sapiens
124 FOXK1 221937
Affinity Capture-MS Homo sapiens
125 PXK 54899
Affinity Capture-MS Homo sapiens
126 DRD2  
Affinity Capture-MS Homo sapiens
127 ZNF574  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 PAFAH1B1 5048
Proximity Label-MS Homo sapiens
129 RELA 5970
Co-fractionation Homo sapiens
130 KIF2A 3796
Proximity Label-MS Homo sapiens
131 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
132 ANKFY1 51479
Proximity Label-MS Homo sapiens
133 FAM175B 23172
Proximity Label-MS Homo sapiens
134 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 USP49 25862
Affinity Capture-MS Homo sapiens
136 DFNB31  
Affinity Capture-MS Homo sapiens
137 MCAT 27349
Affinity Capture-MS Homo sapiens
138 RTN3 10313
Co-fractionation Homo sapiens
139 MTMR3  
Proximity Label-MS Homo sapiens
140 EZR 7430
Proximity Label-MS Homo sapiens
141 TNFSF14  
Affinity Capture-MS Homo sapiens
142 INCA1  
Two-hybrid Homo sapiens
143 ZNF276 92822
Affinity Capture-MS Homo sapiens
144 SKP1 6500
Co-fractionation Homo sapiens
145 SEPT10 151011
Proximity Label-MS Homo sapiens
146 DCTN2 10540
Proximity Label-MS Homo sapiens
147 GAB2 9846
Affinity Capture-MS Homo sapiens
148 KCNV2  
Two-hybrid Homo sapiens
149 LATS1  
Proximity Label-MS Homo sapiens
150 Ppp6r1  
Affinity Capture-MS Mus musculus
151 THAP7  
Two-hybrid Homo sapiens
152 UFL1 23376
Affinity Capture-MS Homo sapiens
153 TBK1 29110
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
154 PPP1R9A  
Proximity Label-MS Homo sapiens
155 ZNRF2 223082
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
156 MAGED1 9500
Proximity Label-MS Homo sapiens
157 INF2 64423
Affinity Capture-MS Homo sapiens
158 CEP135  
Proximity Label-MS Homo sapiens
159 PLEKHG1  
Proximity Label-MS Homo sapiens
160 ACACB 32
Proximity Label-MS Homo sapiens
161 BRD1 23774
Affinity Capture-MS Homo sapiens
162 XRCC6 2547
Affinity Capture-MS Homo sapiens
163 ANK3  
Proximity Label-MS Homo sapiens
164 MOB1A 55233
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
165 C17orf59 54785
Proximity Label-MS Homo sapiens
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