Gene description for PPIP5K2
Gene name diphosphoinositol pentakisphosphate kinase 2
Gene symbol PPIP5K2
Other names/aliases HISPPD1
IP7K2
VIP2
Species Homo sapiens
 Database cross references - PPIP5K2
ExoCarta ExoCarta_23262
Vesiclepedia VP_23262
Entrez Gene 23262
HGNC 29035
MIM 611648
UniProt O43314  
 PPIP5K2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PPIP5K2
Molecular Function
    inositol-1,3,4,5,6-pentakisphosphate kinase activity GO:0000827 ISS
    inositol hexakisphosphate kinase activity GO:0000828 IBA
    inositol hexakisphosphate kinase activity GO:0000828 IDA
    inositol hexakisphosphate kinase activity GO:0000828 TAS
    diphosphoinositol pentakisphosphate kinase activity GO:0000829 IBA
    diphosphoinositol pentakisphosphate kinase activity GO:0000829 TAS
    inositol hexakisphosphate 5-kinase activity GO:0000832 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    5-diphosphoinositol pentakisphosphate 1-kinase activity GO:0033857 IBA
    5-diphosphoinositol pentakisphosphate 1-kinase activity GO:0033857 IDA
    5-diphosphoinositol pentakisphosphate 1-kinase activity GO:0033857 ISS
    inositol hexakisphosphate 1-kinase activity GO:0052723 IEA
Biological Process
    inositol metabolic process GO:0006020 IBA
    inositol metabolic process GO:0006020 ISS
    sensory perception of sound GO:0007605 IEA
    inositol phosphate biosynthetic process GO:0032958 IBA
    inositol phosphate metabolic process GO:0043647 TAS
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PPIP5K2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPIP5K2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 SEC24B 10427
Affinity Capture-MS Homo sapiens
3 HNRNPCL3 649330
Cross-Linking-MS (XL-MS) Homo sapiens
4 CETN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 IL17RA 23765
Affinity Capture-MS Homo sapiens
6 WDR6 11180
Co-fractionation Homo sapiens
7 MAD2L1 4085
Affinity Capture-MS Homo sapiens
8 AAK1 22848
Affinity Capture-MS Homo sapiens
9 PLEC 5339
Affinity Capture-MS Homo sapiens
10 URGCP 55665
Affinity Capture-MS Homo sapiens
11 BAG2 9532
Affinity Capture-MS Homo sapiens
12 VAC14 55697
Co-fractionation Homo sapiens
13 SMARCC1 6599
Two-hybrid Homo sapiens
14 AHNAK 79026
Affinity Capture-MS Homo sapiens
15 ARFGAP3 26286
Affinity Capture-MS Homo sapiens
16 NUP88 4927
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 YWHAZ 7534
Affinity Capture-MS Homo sapiens
18 MECP2 4204
Affinity Capture-MS Homo sapiens
19 THOC7 80145
Co-fractionation Homo sapiens
20 HNRNPCL1 343069
Cross-Linking-MS (XL-MS) Homo sapiens
21 LUZP1 7798
Affinity Capture-MS Homo sapiens
22 COPE 11316
Affinity Capture-MS Homo sapiens
23 DNMBP 23268
Affinity Capture-MS Homo sapiens
24 PIAS4  
Affinity Capture-MS Homo sapiens
25 COPA 1314
Affinity Capture-MS Homo sapiens
26 DUSP16  
Affinity Capture-MS Homo sapiens
27 ANKRD28 23243
Affinity Capture-MS Homo sapiens
28 HNRNPCL2 440563
Cross-Linking-MS (XL-MS) Homo sapiens
29 POU2F1 5451
Two-hybrid Homo sapiens
30 CELA3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 UBE2D2 7322
Affinity Capture-MS Homo sapiens
32 POU6F2  
Two-hybrid Homo sapiens
33 EYA4 2070
Affinity Capture-MS Homo sapiens
34 RPA3 6119
Proximity Label-MS Homo sapiens
35 NTRK1 4914
Affinity Capture-MS Homo sapiens
36 WDR74 54663
Co-fractionation Homo sapiens
37 COPB2 9276
Affinity Capture-MS Homo sapiens
38 HSPA1A 3303
Affinity Capture-MS Homo sapiens
39 BICD2 23299
Proximity Label-MS Homo sapiens
40 YWHAQ 10971
Affinity Capture-MS Homo sapiens
41 PTGES3 10728
Affinity Capture-MS Homo sapiens
42 CEP192 55125
Co-fractionation Homo sapiens
43 YWHAG 7532
Affinity Capture-MS Homo sapiens
44 CCDC88A 55704
Affinity Capture-MS Homo sapiens
45 SPANXN2  
Affinity Capture-MS Homo sapiens
46 DEF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ZC3H15 55854
Co-fractionation Homo sapiens
48 IL3RA  
Affinity Capture-MS Homo sapiens
49 ZBTB8B  
Co-fractionation Homo sapiens
50 YWHAB 7529
Affinity Capture-MS Homo sapiens
51 CAPN2 824
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPIP5K2 is involved
PathwayEvidenceSource
Inositol phosphate metabolism TAS Reactome
Metabolism TAS Reactome
Synthesis of pyrophosphates in the cytosol TAS Reactome





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