ExoCarta: Gene summary

Credits | Contact

Gene description for CEP192
Gene name	centrosomal protein 192kDa
Gene symbol	CEP192
Other names/aliases	PPP1R62
Species	Homo sapiens
Database cross references - CEP192
ExoCarta	ExoCarta_55125
Vesiclepedia	VP_55125
Entrez Gene	55125
HGNC	25515
CEP192 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for CEP192

Molecular Function
	protein binding	GO:0005515	IPI
	phosphatase binding	GO:0019902	IDA
Biological Process
	centrosome cycle	GO:0007098	IBA
	centriole replication	GO:0007099	NAS
	response to bacterium	GO:0009617	IEA
	protein localization to centrosome	GO:0071539	IBA
	protein localization to centrosome	GO:0071539	IMP
	centrosome-templated microtubule nucleation	GO:0090222	IMP
	mitotic spindle assembly	GO:0090307	IBA
	mitotic spindle assembly	GO:0090307	IMP
Subcellular Localization
	pericentriolar material	GO:0000242	IBA
	cytoplasm	GO:0005737	IBA
	centrosome	GO:0005813	IDA
	centriole	GO:0005814	IBA
	centriole	GO:0005814	IDA
	cytosol	GO:0005829	TAS
	procentriole	GO:0120098	IDA
	procentriole replication complex	GO:0120099	IPI

Experiment description of studies that identified CEP192 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for CEP192

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

HOMER3

9454

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FLOT2

2319

Affinity Capture-MS

Homo sapiens

AP2B1

163

Proximity Label-MS

Homo sapiens

HOOK1

Proximity Label-MS

Homo sapiens

GMNN

Affinity Capture-MS

Homo sapiens

ETV6

Proximity Label-MS

Homo sapiens

ALMS1

Affinity Capture-MS

Homo sapiens

RABEP2

79874

Affinity Capture-MS

Homo sapiens

SEPT10

151011

Proximity Label-MS

Homo sapiens

RPA2

6118

Proximity Label-MS

Homo sapiens

KPNB1

3837

Affinity Capture-MS

Homo sapiens

UBC

7316

Reconstituted Complex

Homo sapiens

EYA2

Proximity Label-MS

Homo sapiens

LOXL4

84171

Affinity Capture-MS

Homo sapiens

ANKRD28

23243

Proximity Label-MS

Homo sapiens

CARD8

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Pip4k2c

Affinity Capture-MS

Mus musculus

PCNT

Proximity Label-MS

Homo sapiens

MLLT4

4301

Proximity Label-MS

Homo sapiens

ACTR3

10096

Proximity Label-MS

Homo sapiens

CCDC14

Proximity Label-MS

Homo sapiens

AMOT

Proximity Label-MS

Homo sapiens

LAMTOR1

55004

Affinity Capture-MS

Homo sapiens

Erp44

76299

Affinity Capture-MS

Mus musculus

NINL

Proximity Label-MS

Homo sapiens

NUP155

9631

Proximity Label-MS

Homo sapiens

PFN1

5216

Proximity Label-MS

Homo sapiens

AURKA

6790

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PPP1CA

5499

Reconstituted Complex

Homo sapiens

POLD1

5424

Affinity Capture-MS

Homo sapiens

RBM12B

Affinity Capture-MS

Homo sapiens

PPIP5K2

23262

Co-fractionation

Homo sapiens

Sept11

Affinity Capture-MS

Mus musculus

BICD2

23299

Proximity Label-MS

Homo sapiens

DHFRL1

Proximity Label-MS

Homo sapiens

ANXA6

309

Affinity Capture-MS

Homo sapiens

STIL

Proximity Label-MS

Homo sapiens

AGPS

8540

Proximity Label-MS

Homo sapiens

LATS2

26524

Proximity Label-MS

Homo sapiens

CYLD

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

PIPSL

266971

Affinity Capture-MS

Homo sapiens

CETN1

Co-localization

Homo sapiens

S100P

6286

Affinity Capture-MS

Homo sapiens

SKP2

Affinity Capture-Western

Homo sapiens

CEP135

Proximity Label-MS

Homo sapiens

DNAJC2

27000

Proximity Label-MS

Homo sapiens

PCDHB15

Affinity Capture-MS

Homo sapiens

SDCCAG8

Affinity Capture-MS

Homo sapiens

CUL3

8452

Affinity Capture-MS

Homo sapiens

BNIP3L

Affinity Capture-MS

Homo sapiens

HOOK3

84376

Proximity Label-MS

Homo sapiens

KIAA1524

57650

Two-hybrid

Homo sapiens

DUSP16

Affinity Capture-MS

Homo sapiens

SASS6

163786

Proximity Label-MS

Homo sapiens

NXF1

10482

Affinity Capture-RNA

Homo sapiens

CDK5RAP2

55755

Proximity Label-MS

Homo sapiens

MYO6

4646

Proximity Label-MS

Homo sapiens

RPA3

6119

Proximity Label-MS

Homo sapiens

NIN

51199

Proximity Label-MS

Homo sapiens

GOLGA1

Proximity Label-MS

Homo sapiens

Cep78

Affinity Capture-MS

Mus musculus

NDC80

10403

Proximity Label-MS

Homo sapiens

BICD1

636

Proximity Label-MS

Homo sapiens

ENO1

2023

Affinity Capture-RNA

Homo sapiens

DNAJB13

Proximity Label-MS

Homo sapiens

PCM1

5108

Proximity Label-MS

Homo sapiens

KRT19

3880

Proximity Label-MS

Homo sapiens

DCTPP1

79077

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ACBD5

91452

Proximity Label-MS

Homo sapiens

FTL

2512

Affinity Capture-MS

Homo sapiens

KRT18

3875

Proximity Label-MS

Homo sapiens

FEZ1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KIF2A

3796

Affinity Capture-MS

Homo sapiens

Mkln1

Affinity Capture-MS

Mus musculus

SLMAP

7871

Proximity Label-MS

Homo sapiens

LINC00839

Protein-RNA

Homo sapiens

KDM1B

Affinity Capture-MS

Homo sapiens

SIL1

64374

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FAM184A

79632

Proximity Label-MS

Homo sapiens

S100A10

6281

Affinity Capture-MS

Homo sapiens

DCTN2

10540

Proximity Label-MS

Homo sapiens

PJA2

Affinity Capture-MS

Homo sapiens

ZBTB10

65986

Affinity Capture-MS

Homo sapiens

LATS1

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

ANAPC2

29882

Proximity Label-MS

Homo sapiens

HECTD3

79654

Affinity Capture-MS

Homo sapiens

TRIM37

Proximity Label-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

MOV10

4343

Affinity Capture-RNA

Homo sapiens

EZH2

Affinity Capture-MS

Homo sapiens

EGLN2

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

KRT8

3856

Proximity Label-MS

Homo sapiens

NEDD1

121441

Affinity Capture-MS

Homo sapiens

PXN

5829

Proximity Label-MS

Homo sapiens

CLTA

1211

Proximity Label-MS

Homo sapiens

DYNLRB1

83658

Proximity Label-MS

Homo sapiens

CEP85

Affinity Capture-MS

Homo sapiens

DIAPH1

1729

Affinity Capture-MS

Homo sapiens

C17orf59

54785

Proximity Label-MS

Homo sapiens

RNF41

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which CEP192 is involved

Pathway	Evidence	Source
Anchoring of the basal body to the plasma membrane	TAS	Reactome
AURKA Activation by TPX2	TAS	Reactome
Cell Cycle	TAS	Reactome
Cell Cycle, Mitotic	TAS	Reactome
Centrosome maturation	TAS	Reactome
Cilium Assembly	TAS	Reactome
G2/M Transition	TAS	Reactome
Loss of Nlp from mitotic centrosomes	TAS	Reactome
Loss of proteins required for interphase microtubule organization from the centrosome	TAS	Reactome
M Phase	TAS	Reactome
Mitotic G2-G2/M phases	TAS	Reactome
Mitotic Prometaphase	TAS	Reactome
Organelle biogenesis and maintenance	TAS	Reactome
Recruitment of mitotic centrosome proteins and complexes	TAS	Reactome
Recruitment of NuMA to mitotic centrosomes	TAS	Reactome
Regulation of PLK1 Activity at G2/M Transition	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here