Gene description for DYNLRB1
Gene name dynein, light chain, roadblock-type 1
Gene symbol DYNLRB1
Other names/aliases BITH
BLP
DNCL2A
DNLC2A
ROBLD1
Species Homo sapiens
 Database cross references - DYNLRB1
ExoCarta ExoCarta_83658
Entrez Gene 83658
HGNC 15468
MIM 607167
UniProt Q9NP97  
 DYNLRB1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for DYNLRB1
Molecular Function
    protein binding GO:0005515 IPI
    microtubule motor activity GO:0003777 NAS
Biological Process
    transport GO:0006810 IEA
    organelle organization GO:0006996 TAS
    metabolic process GO:0008152 NAS
    microtubule-based movement GO:0007018 NAS
    visual behavior GO:0007632 ISS
Subcellular Localization
    microtubule GO:0005874 IEA
    cytoplasm GO:0005737 ISS
    cytoplasmic dynein complex GO:0005868 NAS
    centrosome GO:0005813 IDA
    membrane GO:0016020 IDA
    ciliary tip GO:0097542 TAS
    primary cilium GO:0072372 TAS
 Experiment description of studies that identified DYNLRB1 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DYNLRB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPHOSPH6  
Affinity Capture-MS Homo sapiens
2 HSPH1 10808
Two-hybrid Homo sapiens
3 DNCI1  
Two-hybrid Homo sapiens
4 MPP6 51678
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DYNLRB1 is involved
PathwayEvidenceSource
Intraflagellar transport TAS Reactome





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