Gene description for DYNLRB1
Gene name dynein, light chain, roadblock-type 1
Gene symbol DYNLRB1
Other names/aliases BITH
BLP
DNCL2A
DNLC2A
ROBLD1
Species Homo sapiens
 Database cross references - DYNLRB1
ExoCarta ExoCarta_83658
Vesiclepedia VP_83658
Entrez Gene 83658
HGNC 15468
MIM 607167
UniProt Q9NP97  
 DYNLRB1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for DYNLRB1
Molecular Function
    microtubule motor activity GO:0003777 NAS
    protein binding GO:0005515 IPI
    dynein intermediate chain binding GO:0045505 IBA
Biological Process
    microtubule-based movement GO:0007018 IBA
    microtubule-based movement GO:0007018 NAS
    visual behavior GO:0007632 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    cytoplasmic dynein complex GO:0005868 IBA
    cytoplasmic dynein complex GO:0005868 ISS
    cytoplasmic dynein complex GO:0005868 NAS
    microtubule GO:0005874 IEA
    cilium GO:0005929 TAS
    membrane GO:0016020 HDA
    dynein complex GO:0030286 IPI
    ciliary tip GO:0097542 TAS
 Experiment description of studies that identified DYNLRB1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DYNLRB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TCTEX1D2  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 MRPS26 64949
Co-fractionation Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 NUDC 10726
Affinity Capture-Western Homo sapiens
6 C9orf72  
Affinity Capture-MS Homo sapiens
7 MYCN  
Affinity Capture-MS Homo sapiens
8 WDR34 89891
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
9 DYNLL2 140735
Affinity Capture-MS Homo sapiens
10 MPP6 51678
Two-hybrid Homo sapiens
11 CLIP1 6249
Affinity Capture-MS Homo sapiens
12 DYNC2LI1 51626
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
13 DYNLT1 6993
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
14 Dctn1 13191
Affinity Capture-MS Mus musculus
15 AKAP12 9590
Co-fractionation Homo sapiens
16 SUGT1 10910
Proximity Label-MS Homo sapiens
17 DYNC1I2 1781
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
18 CHMP4C 92421
Affinity Capture-MS Homo sapiens
19 CENPJ 55835
Affinity Capture-MS Homo sapiens
20 Dync1i2  
Affinity Capture-MS Mus musculus
21 DYNLRB1 83658
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
22 ELL  
Proximity Label-MS Homo sapiens
23 DYNC1I1  
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TUBB4B 10383
Affinity Capture-MS Homo sapiens
26 PIK3AP1  
Proximity Label-MS Homo sapiens
27 DCTN2 10540
Co-fractionation Homo sapiens
28 OSGEP 55644
Proximity Label-MS Homo sapiens
29 PLEKHG2 64857
Proximity Label-MS Homo sapiens
30 CAPZB 832
Affinity Capture-MS Homo sapiens
31 ELP6 54859
Proximity Label-MS Homo sapiens
32 ZNF507  
Proximity Label-MS Homo sapiens
33 MORC3 23515
Proximity Label-MS Homo sapiens
34 CEP192 55125
Proximity Label-MS Homo sapiens
35 RAB21 23011
Proximity Label-MS Homo sapiens
36 SEPT2 4735
Co-fractionation Homo sapiens
37 MAGEA2  
Two-hybrid Homo sapiens
38 Dync1li1 235661
Affinity Capture-MS Mus musculus
39 MAPRE1 22919
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HSPA5 3309
Cross-Linking-MS (XL-MS) Homo sapiens
41 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
42 SAFB 6294
Co-fractionation Homo sapiens
43 CHCHD4  
Affinity Capture-MS Homo sapiens
44 WDR60 55112
Proximity Label-MS Homo sapiens
45 UNK  
Proximity Label-MS Homo sapiens
46 CLASP1 23332
Affinity Capture-MS Homo sapiens
47 PLOD3 8985
Co-fractionation Homo sapiens
48 Dync1h1 13424
Affinity Capture-MS Mus musculus
49 DRG2 1819
Proximity Label-MS Homo sapiens
50 MYC  
Affinity Capture-MS Homo sapiens
51 Dctn3  
Affinity Capture-MS Mus musculus
52 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
53 DYNC1LI2 1783
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
54 Pafah1b1 18472
Affinity Capture-MS Mus musculus
55 LOC102724023  
Proximity Label-MS Homo sapiens
56 LAMTOR2 28956
Co-fractionation Homo sapiens
57 MPHOSPH8 54737
Affinity Capture-MS Homo sapiens
58 SMG7  
Proximity Label-MS Homo sapiens
59 DCTN1 1639
Affinity Capture-Western Homo sapiens
60 FLNC 2318
Co-fractionation Homo sapiens
61 KIF11 3832
Affinity Capture-MS Homo sapiens
62 MAGEA2B  
Two-hybrid Homo sapiens
63 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
64 Dynll1 56455
Affinity Capture-MS Mus musculus
65 HSPH1 10808
Two-hybrid Homo sapiens
66 EIF4ENIF1 56478
Proximity Label-MS Homo sapiens
67 DBT 1629
Proximity Label-MS Homo sapiens
68 CCDC88A 55704
Affinity Capture-MS Homo sapiens
69 KRT19 3880
Proximity Label-MS Homo sapiens
70 Cenpe  
Affinity Capture-MS Mus musculus
71 CEBPA  
Protein-peptide Homo sapiens
72 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
73 ARHGAP5 394
Proximity Label-MS Homo sapiens
74 CRCP  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DYNLRB1 is involved
PathwayEvidenceSource
Cilium Assembly TAS Reactome
Intraflagellar transport TAS Reactome
Organelle biogenesis and maintenance TAS Reactome





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