Gene description for OSGEP
Gene name O-sialoglycoprotein endopeptidase
Gene symbol OSGEP
Other names/aliases GCPL1
KAE1
OSGEP1
PRSMG1
Species Homo sapiens
 Database cross references - OSGEP
ExoCarta ExoCarta_55644
Vesiclepedia VP_55644
Entrez Gene 55644
HGNC 18028
MIM 610107
UniProt Q9NPF4  
 OSGEP identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for OSGEP
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    N(6)-L-threonylcarbamoyladenine synthase activity GO:0061711 IDA
Biological Process
    tRNA threonylcarbamoyladenosine modification GO:0002949 IDA
    tRNA threonylcarbamoyladenosine modification GO:0002949 IMP
    tRNA modification GO:0006400 IDA
Subcellular Localization
    EKC/KEOPS complex GO:0000408 IBA
    EKC/KEOPS complex GO:0000408 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified OSGEP in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for OSGEP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RYK 6259
Affinity Capture-MS Homo sapiens
3 ACTR2 10097
Co-fractionation Homo sapiens
4 F11R 50848
Affinity Capture-MS Homo sapiens
5 SPRTN  
Affinity Capture-MS Homo sapiens
6 TCEB1 6921
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 GATSL2  
Co-fractionation Homo sapiens
8 SHC1 6464
Two-hybrid Homo sapiens
9 DIS3 22894
Affinity Capture-MS Homo sapiens
10 TPD52L2 7165
Co-fractionation Homo sapiens
11 ZPR1 8882
Co-fractionation Homo sapiens
12 C14orf142  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PBK  
Affinity Capture-MS Homo sapiens
14 EFTUD1 79631
Co-fractionation Homo sapiens
15 ILF2 3608
Co-fractionation Homo sapiens
16 CRIP2 1397
Two-hybrid Homo sapiens
17 RBM48  
Two-hybrid Homo sapiens
18 PPP1R12A 4659
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
19 FTO 79068
Affinity Capture-MS Homo sapiens
20 NDRG1 10397
Affinity Capture-MS Homo sapiens
21 CIAPIN1 57019
Two-hybrid Homo sapiens
22 LENG1  
Two-hybrid Homo sapiens
23 TBC1D23 55773
Co-fractionation Homo sapiens
24 PPP1R7 5510
Co-fractionation Homo sapiens
25 RHOA 387
Affinity Capture-MS Homo sapiens
26 TOM1L1 10040
Co-fractionation Homo sapiens
27 HPS6 79803
Two-hybrid Homo sapiens
28 P3H1 64175
Co-fractionation Homo sapiens
29 ALCAM 214
Affinity Capture-MS Homo sapiens
30 WIZ 58525
Two-hybrid Homo sapiens
31 MTMR10 54893
Affinity Capture-MS Homo sapiens
32 NIF3L1 60491
Co-fractionation Homo sapiens
33 TES 26136
Co-fractionation Homo sapiens
34 ZNF579  
Two-hybrid Homo sapiens
35 C19orf60  
Two-hybrid Homo sapiens
36 PPM1B 5495
Affinity Capture-MS Homo sapiens
37 NSUN2 54888
Co-fractionation Homo sapiens
38 SRRT 51593
Affinity Capture-MS Homo sapiens
39 DBNL 28988
Affinity Capture-MS Homo sapiens
40 NAA50 80218
Affinity Capture-MS Homo sapiens
41 RAB14 51552
Affinity Capture-MS Homo sapiens
42 SAE1 10055
Co-fractionation Homo sapiens
43 ANO4 121601
Affinity Capture-MS Homo sapiens
44 PANK4 55229
Co-fractionation Homo sapiens
45 VRK1 7443
Affinity Capture-MS Homo sapiens
46 SP110  
Two-hybrid Homo sapiens
47 RPA2 6118
Proximity Label-MS Homo sapiens
48 CKAP5 9793
Affinity Capture-MS Homo sapiens
49 RAP1A 5906
Affinity Capture-MS Homo sapiens
50 DNAJA1 3301
Affinity Capture-MS Homo sapiens
51 TPRKB 51002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SMS 6611
Affinity Capture-MS Homo sapiens
53 CUL2 8453
Affinity Capture-Western Homo sapiens
54 FAM49B 51571
Affinity Capture-MS Homo sapiens
55 ENAH 55740
Affinity Capture-MS Homo sapiens
56 WDFY1 57590
Affinity Capture-MS Homo sapiens
57 WDR82 80335
Two-hybrid Homo sapiens
58 HSPA5 3309
Co-fractionation Homo sapiens
59 TOMM34 10953
Affinity Capture-MS Homo sapiens
60 HSPA9 3313
Co-fractionation Homo sapiens
61 CCDC28B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 PNPT1 87178
Affinity Capture-MS Homo sapiens
63 MBIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 LETM1 3954
Affinity Capture-MS Homo sapiens
65 LAMP1 3916
Co-fractionation Homo sapiens
66 NR3C1 2908
Two-hybrid Homo sapiens
67 OSGEP 55644
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 NUP43 348995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 HNRNPUL1 11100
Co-fractionation Homo sapiens
70 ERP44 23071
Co-fractionation Homo sapiens
71 LIN7A 8825
Affinity Capture-MS Homo sapiens
72 KIF1BP 26128
Co-fractionation Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 PKP4 8502
Two-hybrid Homo sapiens
75 PDE4DIP 9659
Two-hybrid Homo sapiens
76 TP53RK 112858
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
77 TCEB2 6923
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
78 HDLBP 3069
Affinity Capture-MS Homo sapiens
79 FKBP3 2287
Affinity Capture-MS Homo sapiens
80 SF3B3 23450
Two-hybrid Homo sapiens
81 TKFC 26007
Affinity Capture-MS Homo sapiens
82 IRF6 3664
Co-fractionation Homo sapiens
83 DPYSL3 1809
Affinity Capture-MS Homo sapiens
84 EEF2K  
Co-fractionation Homo sapiens
85 C9orf72  
Affinity Capture-MS Homo sapiens
86 NAA10 8260
Co-fractionation Homo sapiens
87 VPS25 84313
Co-fractionation Homo sapiens
88 EHD1 10938
Affinity Capture-MS Homo sapiens
89 POP7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 MAP2K2 5605
Affinity Capture-MS Homo sapiens
91 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
92 STK24 8428
Affinity Capture-MS Homo sapiens
93 POTEF 728378
Affinity Capture-MS Homo sapiens
94 CALU 813
Co-fractionation Homo sapiens
95 EMCN  
Biochemical Activity Homo sapiens
96 TNS1 7145
Two-hybrid Homo sapiens
97 EPHX1 2052
Affinity Capture-MS Homo sapiens
98 CYTH4  
Affinity Capture-MS Homo sapiens
99 OTUB1 55611
Affinity Capture-MS Homo sapiens
100 MAP2K1 5604
Affinity Capture-MS Homo sapiens
101 PRAME  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
102 IDH2 3418
Affinity Capture-MS Homo sapiens
103 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
104 PIN1 5300
Affinity Capture-MS Homo sapiens
105 NFYB  
Co-fractionation Homo sapiens
106 API5 8539
Co-fractionation Homo sapiens
107 LAGE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
108 QRICH1  
Affinity Capture-MS Homo sapiens
109 HYKK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 ZNF775  
Two-hybrid Homo sapiens
111 DYNLRB1 83658
Proximity Label-MS Homo sapiens
112 HNRNPF 3185
Co-fractionation Homo sapiens
113 RPRD1B 58490
Co-fractionation Homo sapiens
114 GLRX3 10539
Co-fractionation Homo sapiens
115 KRAS 3845
Negative Genetic Homo sapiens
116 RAB5C 5878
Affinity Capture-MS Homo sapiens
117 POLR1D 51082
Affinity Capture-MS Homo sapiens
118 SPRED1 161742
Two-hybrid Homo sapiens
119 BAG1 573
Co-fractionation Homo sapiens
120 NVL  
Two-hybrid Homo sapiens
121 RANGAP1 5905
Co-fractionation Homo sapiens
122 UFM1 51569
Co-fractionation Homo sapiens
123 NAP1L5  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which OSGEP is involved
PathwayEvidenceSource
Metabolism of RNA IEA Reactome
tRNA modification in the nucleus and cytosol IEA Reactome
tRNA processing IEA Reactome





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