Gene description for EPHX1
Gene name epoxide hydrolase 1, microsomal (xenobiotic)
Gene symbol EPHX1
Other names/aliases EPHX
EPOX
HYL1
MEH
Species Homo sapiens
 Database cross references - EPHX1
ExoCarta ExoCarta_2052
Vesiclepedia VP_2052
Entrez Gene 2052
HGNC 3401
MIM 132810
UniProt P07099  
 EPHX1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for EPHX1
Molecular Function
    epoxide hydrolase activity GO:0004301 IBA
    epoxide hydrolase activity GO:0004301 IDA
    protein binding GO:0005515 IPI
    oxysterol binding GO:0008142 ISS
    cis-stilbene-oxide hydrolase activity GO:0033961 IDA
    cis-stilbene-oxide hydrolase activity GO:0033961 TAS
Biological Process
    xenobiotic metabolic process GO:0006805 TAS
    response to toxic substance GO:0009636 IEA
    arachidonate metabolic process GO:0019369 IBA
    arachidonate metabolic process GO:0019369 IDA
    epoxide metabolic process GO:0097176 IBA
    epoxide metabolic process GO:0097176 IDA
    hydrocarbon catabolic process GO:0120253 IEA
    response to polycyclic arene GO:1903165 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified EPHX1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EPHX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NKAIN1  
Affinity Capture-MS Homo sapiens
2 BCLAF1 9774
Co-fractionation Homo sapiens
3 BRD2  
Affinity Capture-MS Homo sapiens
4 CYP26B1  
Affinity Capture-MS Homo sapiens
5 KCNS3 3790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ISG15 9636
Affinity Capture-MS Homo sapiens
7 CHD8 57680
Co-fractionation Homo sapiens
8 CDC27 996
Co-fractionation Homo sapiens
9 TMED10 10972
Co-fractionation Homo sapiens
10 SHISA2 387914
Affinity Capture-MS Homo sapiens
11 RTN4 57142
Affinity Capture-MS Homo sapiens
12 TMEM30A 55754
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SPRY2 10253
Affinity Capture-MS Homo sapiens
14 FAM20C 56975
Affinity Capture-MS Homo sapiens
15 ASS1 445
Co-fractionation Homo sapiens
16 RXFP1  
Affinity Capture-MS Homo sapiens
17 IFT20 90410
Affinity Capture-MS Homo sapiens
18 PCDHGA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SIDT2 51092
Affinity Capture-MS Homo sapiens
20 NR3C1 2908
Affinity Capture-MS Homo sapiens
21 PCDHGB5 56101
Affinity Capture-MS Homo sapiens
22 KCNC3 3748
Affinity Capture-MS Homo sapiens
23 SRPRB 58477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RAB34 83871
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CCDC107  
Affinity Capture-MS Homo sapiens
26 TFDP2  
Affinity Capture-MS Homo sapiens
27 FAS 355
Proximity Label-MS Homo sapiens
28 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 TMEM9B 56674
Affinity Capture-MS Homo sapiens
30 CDKN2AIP  
Co-fractionation Homo sapiens
31 RNF139  
Affinity Capture-MS Homo sapiens
32 IL22RA1 58985
Affinity Capture-MS Homo sapiens
33 KIAA1429 25962
Affinity Capture-MS Homo sapiens
34 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RGS2  
Affinity Capture-MS Homo sapiens
36 MRPL16  
Co-fractionation Homo sapiens
37 IL17RA 23765
Affinity Capture-MS Homo sapiens
38 MFSD8 256471
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TMX1 81542
Co-fractionation Homo sapiens
40 PARK2  
Affinity Capture-MS Homo sapiens
41 OSGEP 55644
Affinity Capture-MS Homo sapiens
42 IQCF1  
Affinity Capture-MS Homo sapiens
43 MECP2 4204
Affinity Capture-MS Homo sapiens
44 MORF4L1  
Affinity Capture-MS Homo sapiens
45 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 HPDL 84842
Co-fractionation Homo sapiens
47 OPTN 10133
Affinity Capture-MS Homo sapiens
48 NXF1 10482
Affinity Capture-RNA Homo sapiens
49 CCDC53 51019
Affinity Capture-MS Homo sapiens
50 EVA1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 HADHA 3030
Co-fractionation Homo sapiens
52 DHRS13  
Cross-Linking-MS (XL-MS) Homo sapiens
53 HDGFRP2 84717
Co-fractionation Homo sapiens
54 CHRNA9  
Affinity Capture-MS Homo sapiens
55 ZRANB2 9406
Co-fractionation Homo sapiens
56 C3orf52  
Affinity Capture-MS Homo sapiens
57 ABAT  
Affinity Capture-MS Homo sapiens
58 RPA2 6118
Proximity Label-MS Homo sapiens
59 PCDHGC4  
Affinity Capture-MS Homo sapiens
60 VCP 7415
Affinity Capture-MS Homo sapiens
61 ZNRF4  
Affinity Capture-MS Homo sapiens
62 CDK11B 984
Co-fractionation Homo sapiens
63 PCDHB3  
Affinity Capture-MS Homo sapiens
64 CKMT1A 548596
Co-fractionation Homo sapiens
65 FAM189A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 MARCH4  
Affinity Capture-MS Homo sapiens
67 TREML2 79865
Affinity Capture-MS Homo sapiens
68 KCNA2  
Affinity Capture-MS Homo sapiens
69 RNF2  
Affinity Capture-MS Homo sapiens
70 PTTG1IP 754
Co-fractionation Homo sapiens
71 ATP4A 495
Affinity Capture-MS Homo sapiens
72 CHRND  
Affinity Capture-MS Homo sapiens
73 RIOK2 55781
Co-fractionation Homo sapiens
74 PIGH  
Affinity Capture-MS Homo sapiens
75 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 RPA3 6119
Proximity Label-MS Homo sapiens
77 TRAF1 7185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 ESYT1 23344
Affinity Capture-MS Homo sapiens
79 NMUR2  
Affinity Capture-MS Homo sapiens
80 DNAJB11 51726
Co-fractionation Homo sapiens
81 PTAFR  
Affinity Capture-MS Homo sapiens
82 UBE2O 63893
Co-fractionation Homo sapiens
83 DERL1 79139
Affinity Capture-MS Homo sapiens
84 CKMT1B 1159
Co-fractionation Homo sapiens
85 CLRN2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EPHX1 is involved
PathwayEvidenceSource
Biological oxidations TAS Reactome
Metabolism TAS Reactome
Phase I - Functionalization of compounds TAS Reactome





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