Gene description for OPTN
Gene name optineurin
Gene symbol OPTN
Other names/aliases ALS12
FIP2
GLC1E
HIP7
HYPL
NRP
TFIIIA-INTP
Species Homo sapiens
 Database cross references - OPTN
ExoCarta ExoCarta_10133
Vesiclepedia VP_10133
Entrez Gene 10133
HGNC 17142
MIM 602432
UniProt Q96CV9  
 OPTN identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for OPTN
Molecular Function
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IBA
Biological Process
    negative regulation of receptor recycling GO:0001920 IMP
    Golgi organization GO:0007030 IMP
    signal transduction GO:0007165 TAS
    cell death GO:0008219 TAS
    positive regulation of autophagy GO:0010508 IDA
    protein localization to Golgi apparatus GO:0034067 IBA
    protein localization to Golgi apparatus GO:0034067 IMP
    cellular response to unfolded protein GO:0034620 IMP
    Golgi to plasma membrane protein transport GO:0043001 IMP
    regulation of canonical NF-kappaB signal transduction GO:0043122 IBA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IEA
    innate immune response GO:0045087 IEA
    defense response to Gram-negative bacterium GO:0050829 IMP
    type 2 mitophagy GO:0061734 IMP
    Golgi ribbon formation GO:0090161 IBA
    Golgi ribbon formation GO:0090161 IDA
    Golgi ribbon formation GO:0090161 IMP
    positive regulation of xenophagy GO:1904417 IMP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    autophagosome GO:0005776 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    recycling endosome membrane GO:0055038 TAS
 Experiment description of studies that identified OPTN in exosomes
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for OPTN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GABARAPL1 23710
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 MAP1LC3C  
Two-hybrid Homo sapiens
3 GABARAPL2 11345
Reconstituted Complex Homo sapiens
4 TNIP1 10318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 EIF5A2 56648
Affinity Capture-MS Homo sapiens
6 Tfrc 64678
Affinity Capture-Western Rattus norvegicus
7 ATG5 9474
Reconstituted Complex Homo sapiens
8 ATG9A 79065
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
9 EPHX1 2052
Affinity Capture-MS Homo sapiens
10 USP5 8078
Co-fractionation Homo sapiens
11 EPS15 2060
Two-hybrid Homo sapiens
12 TRADD 8717
Affinity Capture-Western Homo sapiens
13 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
14 CALM1 801
Reconstituted Complex Homo sapiens
15 ZNF398  
Two-hybrid Homo sapiens
16 RIPK1 8737
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 ZNF426  
Two-hybrid Homo sapiens
18 MAP1LC3B 81631
Co-localization Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
19 VANGL2  
Affinity Capture-Western Homo sapiens
20 THUMPD1 55623
Affinity Capture-MS Homo sapiens
21 MYH6 4624
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 NDRG1 10397
Affinity Capture-MS Homo sapiens
23 WDR20 91833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 LOC100506753  
Co-fractionation Homo sapiens
Protein-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
25 NFE2L2 4780
Affinity Capture-Western Homo sapiens
26 NRL  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
27 YWHAG 7532
Affinity Capture-MS Homo sapiens
28 NARS 4677
Affinity Capture-MS Homo sapiens
29 RHOA 387
Affinity Capture-MS Homo sapiens
30 DYNLL1 8655
Affinity Capture-MS Homo sapiens
31 TBC1D15 64786
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 CCDC53 51019
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 YWHAH 7533
Affinity Capture-MS Homo sapiens
34 MPP1 4354
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
35 MAVS 57506
Affinity Capture-Western Homo sapiens
36 RAB12 201475
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
37 SDCBP 6386
Two-hybrid Homo sapiens
38 UQCRQ 27089
Reconstituted Complex Homo sapiens
39 TFRC 7037
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RNF11 26994
Two-hybrid Homo sapiens
42 TNF  
Affinity Capture-Western Homo sapiens
43 YWHAB 7529
Affinity Capture-MS Homo sapiens
44 TBC1D17 79735
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
45 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
46 RAB3IL1 5866
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TBK1 29110
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 CDC23 8697
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 RAB14 51552
Far Western Homo sapiens
Two-hybrid Homo sapiens
50 CDKN1A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 PARK2  
Co-localization Homo sapiens
52 PIAS4  
Two-hybrid Homo sapiens
53 BRE 9577
Co-fractionation Homo sapiens
54 OPTN 10133
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
55 HTT 3064
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
56 DAZAP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
57 POU6F2  
Two-hybrid Homo sapiens
58 RAP1B 5908
Affinity Capture-MS Homo sapiens
59 UBA3 9039
Affinity Capture-MS Homo sapiens
60 NUDC 10726
Cross-Linking-MS (XL-MS) Homo sapiens
61 TRIM16 10626
Affinity Capture-MS Homo sapiens
62 WIPI1 55062
Affinity Capture-MS Homo sapiens
63 HACE1 57531
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
64 ZMPSTE24 10269
Affinity Capture-MS Homo sapiens
65 YWHAQ 10971
Affinity Capture-MS Homo sapiens
66 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ATP2B4 493
Affinity Capture-MS Homo sapiens
68 RAB10 10890
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
69 CYLD  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
70 TTLL12 23170
Affinity Capture-MS Homo sapiens
71 GABARAP 11337
Reconstituted Complex Homo sapiens
72 TOMM40 10452
Affinity Capture-MS Homo sapiens
73 SMCHD1 23347
Affinity Capture-MS Homo sapiens
74 TAX1BP1 8887
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
75 FAM178A  
Reconstituted Complex Homo sapiens
76 LETM1 3954
Affinity Capture-MS Homo sapiens
77 RAB25 57111
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
78 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
79 YWHAZ 7534
Affinity Capture-MS Homo sapiens
80 Rab8a 117103
Affinity Capture-Western Rattus norvegicus
81 TRAF3 7187
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
82 ZNF670  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
83 ZMYM6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 GNAS 2778
Reconstituted Complex Homo sapiens
85 MYO6 4646
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
86 JAK3 3718
Affinity Capture-Western Homo sapiens
87 ARRDC3 57561
Two-hybrid Homo sapiens
88 RAB11B 9230
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
89 EPB41 2035
Affinity Capture-MS Homo sapiens
90 HSPA4 3308
Co-fractionation Homo sapiens
91 ZNF384  
Affinity Capture-MS Homo sapiens
92 HSPD1 3329
Co-localization Homo sapiens
93 MPI 4351
Affinity Capture-MS Homo sapiens
94 ZNF17  
Two-hybrid Homo sapiens
95 SOD1 6647
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
96 ZNF329  
Two-hybrid Homo sapiens
97 AIMP1 9255
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
98 MPP6 51678
Affinity Capture-MS Homo sapiens
99 LNX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 IKBKG 8517
Affinity Capture-Western Homo sapiens
101 CASP8 841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
102 LITAF 9516
Two-hybrid Homo sapiens
103 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
104 HSPB1 3315
Two-hybrid Homo sapiens
105 GRM1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
106 SFXN1 94081
Affinity Capture-MS Homo sapiens
107 RAB8A 4218
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
108 WWP2 11060
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
109 MAP1LC3A 84557
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
110 RBM12 10137
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
111 RAB11A 8766
Co-localization Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
112 CCDC13 152206
Two-hybrid Homo sapiens
113 FAM189A2  
Two-hybrid Homo sapiens
114 KCTD17 79734
Affinity Capture-MS Homo sapiens
115 HSF2BP  
Two-hybrid Homo sapiens
116 JAK2 3717
Affinity Capture-Western Homo sapiens
117 Tbk1  
Affinity Capture-Western Mus musculus
118 PTGFRN 5738
Affinity Capture-MS Homo sapiens
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