Gene description for OPTN
Gene name optineurin
Gene symbol OPTN
Other names/aliases ALS12
FIP2
GLC1E
HIP7
HYPL
NRP
TFIIIA-INTP
Species Homo sapiens
 Database cross references - OPTN
ExoCarta ExoCarta_10133
Vesiclepedia VP_10133
Entrez Gene 10133
HGNC 17142
MIM 602432
UniProt Q96CV9  
 OPTN identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for OPTN
Molecular Function
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
    K63-linked polyubiquitin binding GO:0070530 IBA
    polyubiquitin binding GO:0031593 IDA
    Rab GTPase binding GO:0017137 IPI
    protein C-terminus binding GO:0008022 IPI
Biological Process
    regulation of I-kappaB kinase/NF-kappaB signaling GO:0043122 IBA
    defense response to Gram-negative bacterium GO:0050829 IMP
    Golgi ribbon formation GO:0090161 IDA
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IEA
    signal transduction GO:0007165 TAS
    Golgi to plasma membrane protein transport GO:0043001 IMP
    negative regulation of receptor recycling GO:0001920 IMP
    Golgi organization GO:0007030 IMP
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    cell death GO:0008219 TAS
    macroautophagy GO:0016236 IDA
    protein targeting to Golgi GO:0000042 IMP
    mitotic cell cycle GO:0000278 TAS
Subcellular Localization
    recycling endosome GO:0055037 IEA
    nucleus GO:0005634 IBA
    cytoplasmic membrane-bounded vesicle GO:0016023 IBA
    Golgi membrane GO:0000139 TAS
    cytoplasm GO:0005737 IDA
    autophagosome GO:0005776 IEA
    Golgi apparatus GO:0005794 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    nucleoplasm GO:0005654 TAS
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified OPTN in exosomes
1
Experiment ID 76
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
EV Enriched markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for OPTN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HTT 3064
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 FLII 2314
Two-hybrid Homo sapiens
3 RAB8A 4218
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 GTF3A 2971
Invitro Homo sapiens
5 CCDC53 51019
Two-hybrid Homo sapiens
6 RNF11 26994
Affinity Capture-MS Homo sapiens
7 RAB11B 9230
Invivo Homo sapiens
Invitro Homo sapiens
8 TBC1D17 79735
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which OPTN is involved
PathwayEvidenceSource
Regulation of PLK1 Activity at G2/M Transition TAS Reactome





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