Gene description for WIPI1
Gene name WD repeat domain, phosphoinositide interacting 1
Gene symbol WIPI1
Other names/aliases ATG18
ATG18A
WIPI49
Species Homo sapiens
 Database cross references - WIPI1
ExoCarta ExoCarta_55062
Vesiclepedia VP_55062
Entrez Gene 55062
HGNC 25471
MIM 609224
UniProt Q5MNZ9  
 WIPI1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WIPI1
Molecular Function
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
    receptor binding GO:0005102 IDA
    androgen receptor binding GO:0050681 IDA
    estrogen receptor binding GO:0030331 IDA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
Biological Process
    IRE1-mediated unfolded protein response GO:0036498 TAS
    cellular protein metabolic process GO:0044267 TAS
    protein localization to pre-autophagosomal structure GO:0034497 IBA
    cellular response to nitrogen starvation GO:0006995 IBA
    autophagosome assembly GO:0000045 IBA
    protein lipidation GO:0006497 IBA
    mitochondrion degradation GO:0000422 IBA
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    autophagy GO:0006914 IEP
    nucleophagy GO:0044804 IBA
    vesicle targeting, trans-Golgi to endosome GO:0048203 IDA
Subcellular Localization
    cytosol GO:0005829 IBA
    pre-autophagosomal structure GO:0000407 IDA
    endosome membrane GO:0010008 IDA
    trans-Golgi network GO:0005802 IDA
    Golgi membrane GO:0000139 TAS
    clathrin-coated vesicle GO:0030136 IEA
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 IDA
    extrinsic component of membrane GO:0019898 IBA
    autophagosome membrane GO:0000421 IDA
    pre-autophagosomal structure membrane GO:0034045 IDA
 Experiment description of studies that identified WIPI1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WIPI1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KCTD15 79047
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which WIPI1 is involved
PathwayEvidenceSource
XBP1(S) activates chaperone genes TAS Reactome





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