Gene description for AMFR
Gene name autocrine motility factor receptor, E3 ubiquitin protein ligase
Gene symbol AMFR
Other names/aliases GP78
RNF45
Species Homo sapiens
 Database cross references - AMFR
ExoCarta ExoCarta_267
Entrez Gene 267
HGNC 463
MIM 603243
UniProt Q9UKV5  
 AMFR identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for AMFR
Molecular Function
    ubiquitin protein ligase activity GO:0061630 IDA
    receptor activity GO:0004872 IPI
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ligase activity GO:0016874 IEA
    ubiquitin-protein transferase activity GO:0004842 IMP
Biological Process
    movement of cell or subcellular component GO:0006928 TAS
    protein oligomerization GO:0051259 IDA
    aging GO:0007568 IEA
    protein polyubiquitination GO:0000209 IDA
    endoplasmic reticulum unfolded protein response GO:0030968 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    protein K48-linked ubiquitination GO:0070936 IDA
    ER-associated ubiquitin-dependent protein catabolic process GO:0030433 TAS
    signal transduction GO:0007165 TAS
    learning or memory GO:0007611 IEA
Subcellular Localization
    growth cone GO:0030426 IEA
    integral component of endoplasmic reticulum membrane GO:0030176 IDA
    neuronal cell body GO:0043025 IEA
    Hrd1p ubiquitin ligase complex GO:0000836 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    membrane GO:0016020 IDA
    nucleus GO:0005634 IEA
    endoplasmic reticulum membrane GO:0005789 IDA
    integral component of membrane GO:0016021 NAS
    protein complex GO:0043234 IDA
    dendrite GO:0030425 IEA
 Experiment description of studies that identified AMFR in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for AMFR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2D2 7322
Invivo Homo sapiens
Invitro Homo sapiens
2 UBE2G2  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
3 VCP 7415
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which AMFR is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here