Gene description for UBE2K
Gene name ubiquitin-conjugating enzyme E2K
Gene symbol UBE2K
Other names/aliases E2-25K
HIP2
HYPG
LIG
UBC1
Species Homo sapiens
 Database cross references - UBE2K
ExoCarta ExoCarta_3093
Entrez Gene 3093
HGNC 4914
MIM 602846
UniProt P61086  
 UBE2K identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for UBE2K
Molecular Function
    ligase activity GO:0016874 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin-ubiquitin ligase activity GO:0034450 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    protein K48-linked ubiquitination GO:0070936 IDA
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IEA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IBA
    actin cytoskeleton GO:0015629 IDA
    extracellular exosome GO:0070062 IDA
    intermediate filament cytoskeleton GO:0045111 IDA
 Experiment description of studies that identified UBE2K in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBE2K
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 RIPK5  
Affinity Capture-MS Homo sapiens
3 HTT 3064
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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